PMID- 32182810 OWN - NLM STAT- MEDLINE DCOM- 20201204 LR - 20201214 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 21 IP - 5 DP - 2020 Mar 9 TI - Characterization and Evaluation of Resistance to Powdery Mildew of Wheat-Aegilops geniculata Roth 7M(g) (7A) Alien Disomic Substitution Line W16998. LID - 10.3390/ijms21051861 [doi] LID - 1861 AB - Aegilops geniculata Roth has been used as a donor of disease-resistance genes, to enrich the gene pool for wheat (Triticum aestivum) improvement through distant hybridization. In this study, the wheat-Ae. geniculata alien disomic substitution line W16998 was obtained from the BC(1)F(8) progeny of a cross between the common wheat 'Chinese Spring' (CS) and Ae. geniculata Roth (serial number: SY159//CS). This line was identified using cytogenetic techniques, analysis of genomic in situ hybridization (GISH), functional molecular markers (Expressed sequence tag-sequence-tagged site (EST-STS) and PCR-based landmark unique gene (PLUG), fluorescence in situ hybridization (FISH), sequential fluorescence in situ hybridization-genomic in situ hybridization (sequential FISH-GISH), and assessment of agronomic traits and powdery mildew resistance. During the anaphase of meiosis, these were evenly distributed on both sides of the equatorial plate, and they exhibited high cytological stability during the meiotic metaphase and anaphase. GISH analysis indicated that W16998 contained a pair of Ae. geniculata alien chromosomes and 40 common wheat chromosomes. One EST-STS marker and seven PLUG marker results showed that the introduced chromosomes of Ae. geniculata belonged to homoeologous group 7. Nullisomic-tetrasomic analyses suggested that the common wheat chromosome, 7A, was absent in W16998. FISH and sequential FISH-GISH analyses confirmed that the introduced Ae. geniculata chromosome was 7M(g). Therefore, W16998 was a wheat-Ae. geniculata 7M(g) (7A) alien disomic substitution line. Inoculation of isolate E09 (Blumeria graminis f. sp. tritici) in the seedling stage showed that SY159 and W16998 were resistant to powdery mildew, indeed nearly immune, whereas CS was highly susceptible. Compared to CS, W16998 exhibited increased grain weight and more spikelets, and a greater number of superior agronomic traits. Consequently, W16998 was potentially useful. Germplasms transfer new disease-resistance genes and prominent agronomic traits into common wheat, giving the latter some fine properties for breeding. FAU - Wang, Yajuan AU - Wang Y AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China. FAU - Long, Deyu AU - Long D AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. FAU - Wang, Yanzhen AU - Wang Y AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. FAU - Wang, Changyou AU - Wang C AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China. FAU - Liu, Xinlun AU - Liu X AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China. FAU - Zhang, Hong AU - Zhang H AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China. FAU - Tian, Zengrong AU - Tian Z AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China. FAU - Chen, Chunhuan AU - Chen C AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China. FAU - Ji, Wanquan AU - Ji W AUID- ORCID: 0000-0003-0663-4568 AD - College of Agronomy, Northwest A&F University, Yangling 712100, China. AD - State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. AD - Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China. LA - eng GR - 2016YFD0102000/the National Key Research and Development Plan of China/ GR - 31471481/National Natural Science Foundation surface project of China/ GR - 2019NWB036-02-1/Crop Germplasm Resources Protection/ PT - Journal Article DEP - 20200309 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Genetic Markers) SB - IM MH - Aegilops/*genetics MH - Chromosomes, Plant/genetics MH - Cytogenetic Analysis/methods MH - Disease Resistance/*genetics MH - Genes, Plant/*genetics MH - Genetic Markers/genetics MH - Hybridization, Genetic/genetics MH - In Situ Hybridization, Fluorescence/methods MH - Plant Breeding/methods MH - Plant Diseases/genetics MH - Poaceae/genetics MH - Triticum/*genetics PMC - PMC7084935 OTO - NOTNLM OT - Aegilops geniculata Roth OT - alien disomic substitution line OT - molecular cytogenetics OT - powdery mildew resistance. OT - wheat COIS- The authors declare no conflict of interest. EDAT- 2020/03/19 06:00 MHDA- 2020/12/15 06:00 PMCR- 2020/03/01 CRDT- 2020/03/19 06:00 PHST- 2020/02/12 00:00 [received] PHST- 2020/02/29 00:00 [revised] PHST- 2020/03/05 00:00 [accepted] PHST- 2020/03/19 06:00 [entrez] PHST- 2020/03/19 06:00 [pubmed] PHST- 2020/12/15 06:00 [medline] PHST- 2020/03/01 00:00 [pmc-release] AID - ijms21051861 [pii] AID - ijms-21-01861 [pii] AID - 10.3390/ijms21051861 [doi] PST - epublish SO - Int J Mol Sci. 2020 Mar 9;21(5):1861. doi: 10.3390/ijms21051861.