PMID- 32183271
OWN - NLM
STAT- MEDLINE
DCOM- 20210311
LR  - 20210311
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 9
IP  - 3
DP  - 2020 Mar 14
TI  - Glycogen Metabolism Supports Early Glycolytic Reprogramming and Activation in 
      Dendritic Cells in Response to Both TLR and Syk-Dependent CLR Agonists.
LID - 10.3390/cells9030715 [doi]
LID - 715
AB  - Dendritic cells (DCs) increase their metabolic dependence on glucose and 
      glycolysis to support their maturation, activation-associated cytokine 
      production, and T-cell stimulatory capacity. We have previously shown that this 
      increase in glucose metabolism can be initiated by both Toll-like receptor (TLR) 
      and C-type lectin receptor (CLR) agonists. In addition, we have shown that the 
      TLR-dependent demand for glucose is partially satisfied by intracellular glycogen 
      stores. However, the role of glycogen metabolism in supporting CLR-dependent DC 
      glycolytic demand has not been formally demonstrated. In this work, we have shown 
      that DCs activated with fungal-associated beta-glucan ligands exhibit acute 
      glycolysis induction that is dependent on glycogen metabolism. Furthermore, 
      glycogen metabolism supports DC maturation, inflammatory cytokine production, and 
      priming of the nucleotide-binding domain, leucine-rich-containing family, pyrin 
      domain-containing-3 (NLRP3) inflammasome in response to both TLR- and 
      CLR-mediated activation. These data support a model in which different classes of 
      innate immune receptors functionally converge in their requirement for 
      glycogen-dependent glycolysis to metabolically support early DC activation. These 
      studies provide new insight into how DC immune effector function is metabolically 
      regulated in response to diverse inflammatory stimuli.
FAU - Curtis, Kylie D
AU  - Curtis KD
AD  - Undergraduate student researcher or research employee, University of Vermont, 
      Burlington, VT 05405, USA.
FAU - Smith, Portia R
AU  - Smith PR
AD  - Undergraduate student researcher or research employee, University of Vermont, 
      Burlington, VT 05405, USA.
FAU - Despres, Hannah W
AU  - Despres HW
AUID- ORCID: 0000-0003-0321-5202
AD  - Cellular, Molecular, and Biomedical Sciences Graduate Program, University of 
      Vermont, Burlington, VT 05405, USA.
FAU - Snyder, Julia P
AU  - Snyder JP
AD  - Cellular, Molecular, and Biomedical Sciences Graduate Program, University of 
      Vermont, Burlington, VT 05405, USA.
FAU - Hogan, Tyler C
AU  - Hogan TC
AD  - Undergraduate student researcher or research employee, University of Vermont, 
      Burlington, VT 05405, USA.
FAU - Rodriguez, Princess D
AU  - Rodriguez PD
AD  - Cellular, Molecular, and Biomedical Sciences Graduate Program, University of 
      Vermont, Burlington, VT 05405, USA.
FAU - Amiel, Eyal
AU  - Amiel E
AUID- ORCID: 0000-0002-1578-8705
AD  - Department of Biomedical and Health Sciences, University of Vermont, Burlington, 
      VT 05405, USA.
LA  - eng
GR  - P30 GM118228/GM/NIGMS NIH HHS/United States
GR  - T32 AI055402/AI/NIAID NIH HHS/United States
GR  - R21 AI135385/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20200314
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Lectins, C-Type)
RN  - 0 (Toll-Like Receptors)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Dendritic Cells/*metabolism
MH  - Glycogen/*metabolism
MH  - Glycolysis/*immunology
MH  - Humans
MH  - Immunity, Innate/*immunology
MH  - Lectins, C-Type/*metabolism
MH  - Toll-Like Receptors/*metabolism
PMC - PMC7140704
OTO - NOTNLM
OT  - dendritic cells
OT  - glucose
OT  - glycogen
OT  - glycolysis
OT  - innate immunity
OT  - metabolism
COIS- The authors declare no conflict of interest.
EDAT- 2020/03/19 06:00
MHDA- 2021/03/12 06:00
PMCR- 2020/03/01
CRDT- 2020/03/19 06:00
PHST- 2020/01/28 00:00 [received]
PHST- 2020/03/09 00:00 [revised]
PHST- 2020/03/11 00:00 [accepted]
PHST- 2020/03/19 06:00 [entrez]
PHST- 2020/03/19 06:00 [pubmed]
PHST- 2021/03/12 06:00 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - cells9030715 [pii]
AID - cells-09-00715 [pii]
AID - 10.3390/cells9030715 [doi]
PST - epublish
SO  - Cells. 2020 Mar 14;9(3):715. doi: 10.3390/cells9030715.