PMID- 32184620 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220413 IS - 1178-6930 (Print) IS - 1178-6930 (Electronic) IS - 1178-6930 (Linking) VI - 13 DP - 2020 TI - MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4. PG - 1859-1869 LID - 10.2147/OTT.S208024 [doi] AB - BACKGROUND: Recent studies showed that aberrant expression of miRNAs causes tumor-suppressing or promoting effects in various cancers including gastric cancer (GC). Our previous studies showed that lots of miRNAs and mRNA expressed differentially in GC and normal tissues. However, the critical miRNAs and mRNA need to be clarified. MATERIALS AND METHODS: Microarray sequencing was used to profile the differential expression of miRNAs and mRNA in GC and normal tissues. Bioinformatics analysis and database prediction were used to search the critical miRNAs and mRNA. Real-time quantitative polymerase chain reaction (RT-qPCR), luciferase reporter assay, immunohistochemistry (IHC), wound healing assay and transwell assay were used to clarify the relationship between the target miRNAs and mRNA. Statistical analysis was used to seek their value of diagnosis and prognosis. RESULTS: We identified microRNA-6165 (miR-6165) as a novel cancer-related miRNA in GC through high-throughput microarray sequencing. By bioinformatics analysis and luciferase reporter assay, we found STRN4 was the target of miR-6165. Via a series of cell experiments, we determined that miR-6165 suppressed GC cells migration and invasion by targeting STRN4. Also, we discovered the potential diagnosis and prognosis value of miR-6165 and STRN4. CONCLUSION: It was found that miR-6165 might suppress GC migration and invasion by targeting STRN4 in vitro, and the further research should focus more on the potential diagnosis and prognosis value of miR-6165 and STRN4 in gastric cancer patients. CI - (c) 2020 Wang et al. FAU - Wang, Zhu AU - Wang Z AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. AD - Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Li, Yang AU - Li Y AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. AD - Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Cao, Jian AU - Cao J AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Zhang, Wei AU - Zhang W AUID- ORCID: 0000-0002-2001-1052 AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. AD - Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Wang, Quan AU - Wang Q AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. AD - Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Zhang, Zhen AU - Zhang Z AUID- ORCID: 0000-0003-4657-0589 AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. AD - Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Gao, Zhidong AU - Gao Z AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Ye, Yingjiang AU - Ye Y AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. AD - Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Jiang, Kewei AU - Jiang K AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. AD - Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, People's Republic of China. FAU - Wang, Shan AU - Wang S AD - Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China. AD - Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, People's Republic of China. LA - eng PT - Journal Article DEP - 20200303 PL - New Zealand TA - Onco Targets Ther JT - OncoTargets and therapy JID - 101514322 PMC - PMC7060782 OTO - NOTNLM OT - STRN4 OT - gastric cancer OT - miR-6165 OT - migration and invasion COIS- There are no conflicts of interest for the authors to report in this work. EDAT- 2020/03/19 06:00 MHDA- 2020/03/19 06:01 PMCR- 2020/03/03 CRDT- 2020/03/19 06:00 PHST- 2019/03/08 00:00 [received] PHST- 2020/02/11 00:00 [accepted] PHST- 2020/03/19 06:00 [entrez] PHST- 2020/03/19 06:00 [pubmed] PHST- 2020/03/19 06:01 [medline] PHST- 2020/03/03 00:00 [pmc-release] AID - 208024 [pii] AID - 10.2147/OTT.S208024 [doi] PST - epublish SO - Onco Targets Ther. 2020 Mar 3;13:1859-1869. doi: 10.2147/OTT.S208024. eCollection 2020.