PMID- 32186057
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200318
IS  - 2589-451X (Electronic)
IS  - 0255-2922 (Linking)
VI  - 38
IP  - 2
DP  - 2018 Apr
TI  - Effects of Qingshen granules on Janus Kinase/ signal transducer and activator of 
      transcription signaling pathway in rats with unilateral ureteral obstruction.
PG  - 182-189
AB  - OBJECTIVE: To investigate the effects of Qingshen granules (QSG) on janus kinase/ 
      signal transducer and activator of transcription (JAK/STAT) signaling pathway in 
      a rat model of unilateral ureteral obstruction (UUO). METHODS: Sixty male 
      Sprague-Dawley rats were randomly divided into six groups, with 10 animals in 
      each group: the untreated sham-operated normal control group; the untreated UUO 
      model control group, the high dose QSG-treated (16 g?kg-1?d-1) UUO group; the 
      medium dose QSG-treated (8 g?kg-1?d-1) UUO group; the low dose QSG-treated (4 
      g*kg-1*d-1) UUO group; and the valsartan-treated group (20 mg*kg-1*d-1). The two 
      untreated control groups received physiological saline (1 mL/100 g per day). All 
      the rats were sacrificed after a 4-week course of treatment. Serum creatinine and 
      leptin; protein expressions of leptin receptor (OB-R), p-JAK2, p-STAT3, nuclear 
      factors-kBp6 (NF-kBp65), and monocytechemotatic protein-1 (MCP-1); mRNA of JAK2, 
      STAT3, calcium-dependent adhesion (E-cadherin), alpha- smooth muscle actin 
      (|A-SMA) in the kidney tissues; and the expressions of type  cento collagen (Col- cento 
      and fibronectin (FN) and the pathomorphology in kidney tissues were treated. 
      RESULTS: Compared with the normal group, the BUN, Scr, and serum leptin levels 
      and the expressions of MCP-1, p-JAK2, p-STAT3, NF-kBp65 and OB-R in renal 
      tissues, and the mRNA expressions of leptin, JAK2 protein, STAT3 protein, |A-SMA 
      protein in model group were significantly higher (P < 0.01) in the UUO model 
      group. These parameters were significantly reduced in all the QSG-treated groups 
      and the valsartan-treated group than the UUO model group (P < 0.05 or P < 0.01), 
      with the lowest levels found in the medium dose QSG-treated group (P < 0.05). 
      However, the expression levels of E-cadherin, FN, and Col- cento in the renal tissues 
      were contrary to the expressions described above. Severe pathological injury was 
      evident in the renal tissues of UUO model rats, which was alleviated in the 
      QSG-treated and valsartan-treated groups, with the least damage found in the 
      medium dose QSG-treated group. CONCLUSION: Our data suggest that the 
      leptin-mediated JAK/STAT signaling pathway is involved in the process of renal 
      interstitial fibrosis in UUO rats. QSG inhibited the activity of the signaling 
      pathway, reduced the activity of NF-kB and inflammatory effect, and the 
      transdifferentiation in the renal tubular epithelial cells. Treatment with QSG 
      may delay the renal fibrosis and protect the renal function from damage following 
      UUO in rats.
FAU - Wang, Yiping
AU  - Wang Y
AD  - Department of Nephrology, the First Hospital Affiliated to Anhui University of 
      Traditional Chinese Medicine, Hefei 230031, China.
FAU - Wang, Dong
AU  - Wang D
AD  - Department of Nephrology, the First Hospital Affiliated to Anhui University of 
      Traditional Chinese Medicine, Hefei 230031, China.
FAU - Jin, Hua
AU  - Jin H
AD  - Department of Nephrology, the First Hospital Affiliated to Anhui University of 
      Traditional Chinese Medicine, Hefei 230031, China.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Department of Nephrology, the First Hospital Affiliated to Anhui University of 
      Traditional Chinese Medicine, Hefei 230031, China.
FAU - Yu, Min
AU  - Yu M
AD  - Department of Nephrology, the Third Hospital Affiliated to Anhui University of 
      Traditional Chinese Medicine, Hefei 230031, China.
LA  - eng
GR  - 81473673/National Natural Science Foundation of China/
GR  - 81673931/National Natural Science Foundation of China/
GR  - 81403372/National Science Fund for Distinguished Young Scholars of China/
GR  - 1508085MH198/Anhui?Natural?Science Foundation/
PT  - Journal Article
PL  - China
TA  - J Tradit Chin Med
JT  - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
JID - 8211546
SB  - IM
OTO - NOTNLM
OT  - Janus kinases
OT  - Qingshen granules
OT  - Renal fibrosis
OT  - STAT transcription factors
OT  - Signal transduction
EDAT- 2018/04/01 00:00
MHDA- 2018/04/01 00:01
CRDT- 2020/03/19 06:00
PHST- 2020/03/19 06:00 [entrez]
PHST- 2018/04/01 00:00 [pubmed]
PHST- 2018/04/01 00:01 [medline]
AID - 2760 [pii]
PST - ppublish
SO  - J Tradit Chin Med. 2018 Apr;38(2):182-189.