PMID- 32187697
OWN - NLM
STAT- MEDLINE
DCOM- 20210520
LR  - 20210520
IS  - 1751-1097 (Electronic)
IS  - 0031-8655 (Linking)
VI  - 96
IP  - 5
DP  - 2020 Sep
TI  - Fluorescence Intensity Enhancement of Green Carbon Dots: Synthesis, 
      Characterization and Cell Imaging.
PG  - 1032-1040
LID - 10.1111/php.13261 [doi]
AB  - The hydrothermal treatment of green carbon dots (CDs) is an appropriate 
      fluorescent probe synthesis method. CDs are exploited as biological staining 
      agents, especially for cellular detection and imaging. The nitrogen-doped green 
      carbon dots (N-CDs) formation can improve the fluorescence intensity property in 
      a one-step process. Here, we report two N-CDs from lemon and tomato extraction in 
      the presence of hydroxylamine. Lemon and tomato N-CDs showed the blue 
      fluorescence under ultraviolet radiation of about 360 nm. The characterization of 
      CDs and N-CDs showed the presence of N-H and C-N bonds which enhanced the 
      fluorescence efficiency. The mean size of lemon and tomato N-CDs were about 2 and 
      3 nm with an increased quantum yield (QY) of 5% and 3.38%, respectively. The CDs 
      and N-CDs cytotoxicity assay exhibited high cell viability approximately 85% and 
      73%, respectively. N-CDs show superior fluorescent intensity in different 
      solvents and significant stability under long-time UV irradiation, different PH 
      and high ionic strength. Our results indicated that the use of N-CDs in cell 
      imaging can lead to fluorescence intensity enhancement as well as proper 
      biocompatibility. Therefore, the safe and high fluorescence intensity of green 
      N-CDs can be utilized for fluorescent probes in biolabeling and bioimaging 
      applications.
CI  - (c) 2020 American Society for Photobiology.
FAU - Hashemi, Fatemeh
AU  - Hashemi F
AD  - Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz 
      University of Medical Sciences, Tabriz, Iran.
AD  - Hematology and Oncology Research Center, Tabriz University of Medical Sciences, 
      Tabriz, Iran.
FAU - Heidari, Fatemeh
AU  - Heidari F
AD  - Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz 
      University of Medical Sciences, Tabriz, Iran.
AD  - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
FAU - Mohajeri, Nasrin
AU  - Mohajeri N
AUID- ORCID: 0000-0003-0508-746X
AD  - Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz 
      University of Medical Sciences, Tabriz, Iran.
FAU - Mahmoodzadeh, Farideh
AU  - Mahmoodzadeh F
AUID- ORCID: 0000-0002-7354-3466
AD  - Halal Research Center of IRI, FDA, Tehran, Iran.
FAU - Zarghami, Nosratollah
AU  - Zarghami N
AUID- ORCID: 0000-0002-4236-4537
AD  - Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz 
      University of Medical Sciences, Tabriz, Iran.
AD  - Hematology and Oncology Research Center, Tabriz University of Medical Sciences, 
      Tabriz, Iran.
AD  - Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz 
      University of Medical Sciences, Tabriz, Iran.
AD  - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200512
PL  - United States
TA  - Photochem Photobiol
JT  - Photochemistry and photobiology
JID - 0376425
RN  - 0 (Fluorescent Dyes)
RN  - 7440-44-0 (Carbon)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Carbon/*chemistry
MH  - *Fluorescence
MH  - Fluorescent Dyes/chemistry
MH  - HeLa Cells
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - Nanoparticles/*chemistry
MH  - Nitrogen/chemistry
MH  - Spectrophotometry, Ultraviolet
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2020/03/19 06:00
MHDA- 2021/05/21 06:00
CRDT- 2020/03/19 06:00
PHST- 2019/11/08 00:00 [received]
PHST- 2020/02/20 00:00 [accepted]
PHST- 2020/03/19 06:00 [pubmed]
PHST- 2021/05/21 06:00 [medline]
PHST- 2020/03/19 06:00 [entrez]
AID - 10.1111/php.13261 [doi]
PST - ppublish
SO  - Photochem Photobiol. 2020 Sep;96(5):1032-1040. doi: 10.1111/php.13261. Epub 2020 
      May 12.