PMID- 32188348
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20210510
IS  - 2474-0896 (Electronic)
IS  - 0967-4845 (Linking)
VI  - 77
IP  - 3
DP  - 2020 Jul
TI  - Serum LINC00305 expression and its genetic variant rs2850711 are associated with 
      clinical and laboratory features of rheumatoid arthritis.
PG  - 142-147
LID - 10.1080/09674845.2020.1744942 [doi]
AB  - BACKGROUND: Long intergenic non-protein coding (lnc) RNA 00305 (LINC00305) is a 
      pro-inflammatory atherosclerosis-associated lncRNA. We hypothesised that 
      LINC00305 expression and its variant rs2850711 (A/T) are implicated in rheumatoid 
      arthritis (RA) and linked with clinical and routine laboratory markers. METHODS: 
      100 RA patients and 100 healthy controls were recruited. LINC00305 genotyping and 
      expression were performed using allelic-discrimination PCR and quantitative 
      real-time PCR. LINC00305 diagnostic power was evaluated using area under the 
      receiver operating characteristic curve (AUC). Serum nuclear factor- kappaB (NF-kappaB) 
      and matrix metalloproteinase-3 (MMP-3) levels were determined by ELISA, standard 
      laboratory markers by routine methods. RESULTS: LINC00305 expression was 
      significantly increased in RA patients and positively correlated with DAS28, 
      C-reactive protein, erythrocyte sedimentation rate, rheumatoid factor and 
      anti-cyclic citrullinated peptide antibody. In multivariate analysis, NF-kappaB, 
      MMP-3 and LINC00305 were significant predictors of RA (P< 0.0001). Individuals 
      carrying AT and TT genotypes of rs2850711 polymorphism had significantly more 
      likely to have RA than AA genotype carriers (P< 0.05). LINC00305 expression, 
      DAS28 score and serum levels of NF-kappaB and MMP-3 were significantly increased in 
      the patients carrying LINC00305 AT and TT genotypes as compared with AA genotype 
      patients (P< 0.01). CONCLUSION: Increased expression level of LINC00305 and its 
      rs2850711 genetic variant may play a role in the diagnosis and management of RA, 
      and its severity and activity.
FAU - Wahba, A S
AU  - Wahba AS
AUID- ORCID: 0000-0002-2600-9965
AD  - Department of Biochemistry, Faculty of Pharmacy, Suez Canal University , 
      Ismailia, Egypt.
FAU - Ibrahim, M E
AU  - Ibrahim ME
AUID- ORCID: 0000-0003-2577-1111
AD  - Department of Physical Medicine, Rheumatology and Rehabilitation, Faculty of 
      Medicine, Suez Canal University , Ismailia, Egypt.
FAU - Mesbah, N M
AU  - Mesbah NM
AUID- ORCID: 0000-0002-8863-968X
AD  - Department of Biochemistry, Faculty of Pharmacy, Suez Canal University , 
      Ismailia, Egypt.
FAU - Saleh, S M
AU  - Saleh SM
AD  - Department of Biochemistry, Faculty of Pharmacy, Suez Canal University , 
      Ismailia, Egypt.
FAU - Abo-Elmatty, D M
AU  - Abo-Elmatty DM
AUID- ORCID: 0000-0002-6074-2714
AD  - Department of Biochemistry, Faculty of Pharmacy, Suez Canal University , 
      Ismailia, Egypt.
FAU - Mehanna, E T
AU  - Mehanna ET
AUID- ORCID: 0000-0003-2759-2889
AD  - Department of Biochemistry, Faculty of Pharmacy, Suez Canal University , 
      Ismailia, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20200424
PL  - Switzerland
TA  - Br J Biomed Sci
JT  - British journal of biomedical science
JID - 9309208
RN  - 0 (Biomarkers)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Long Noncoding)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Arthritis, Rheumatoid/blood/*genetics
MH  - Biomarkers/*blood
MH  - Blood Sedimentation
MH  - C-Reactive Protein/genetics
MH  - Case-Control Studies
MH  - Female
MH  - Genetic Variation/*genetics
MH  - Humans
MH  - Laboratories
MH  - Male
MH  - Matrix Metalloproteinase 3/genetics
MH  - NF-kappa B/genetics
MH  - RNA, Long Noncoding/*blood/*genetics
MH  - ROC Curve
OTO - NOTNLM
OT  - Rheumatoid arthritis (RA)
OT  - long intergenic non-Protein coding RNA 00305 (LINC00305)
OT  - matrix metalloproteinase-3 (MMP-3)
OT  - nuclear factor- kappaB (NF-kappaB)
EDAT- 2020/03/20 06:00
MHDA- 2021/05/11 06:00
CRDT- 2020/03/20 06:00
PHST- 2020/03/20 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
PHST- 2020/03/20 06:00 [entrez]
AID - 10.1080/09674845.2020.1744942 [pii]
AID - 10.1080/09674845.2020.1744942 [doi]
PST - ppublish
SO  - Br J Biomed Sci. 2020 Jul;77(3):142-147. doi: 10.1080/09674845.2020.1744942. Epub 
      2020 Apr 24.