PMID- 32189836
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220413
IS  - 0972-124X (Print)
IS  - 0975-1580 (Electronic)
IS  - 0972-124X (Linking)
VI  - 24
IP  - 2
DP  - 2020 Mar-Apr
TI  - Early and accurate detection of bacterial isolates from dental plaque in subjects 
      with primary, mixed, and permanent dentition by matrix-assisted laser 
      desorption/ionization time-of-flight mass spectrometry technique.
PG  - 104-108
LID - 10.4103/jisp.jisp_303_19 [doi]
AB  - BACKGROUND: Bacterial colonization of dentition in different age groups can 
      impact prognosis in different dental diseases. Latest diagnostic technique such 
      as matrix-assisted laser desorption/ionization time of flight mass spectrometry 
      (MALDI-TOF) is increasingly being used for accurate identification of bacteria. 
      This study was undertaken to evaluate the MALDI-TOF MS technique to identify 
      bacterial pathogens from dental plaques in subjects with primary, mixed, and 
      permanent dentition. MATERIALS AND METHODS: The study included 150 subjects of 
      different age groups and were divided into three groups - Group A: Subjects with 
      primary dentition (n = 50), Group B: Subjects with mixed dentition (n = 50), and 
      Group C: Subjects with permanent dentition (n = 50). Subgingival dental plaque 
      samples were collected from buccal and lingual surfaces of premolar and molar 
      teeth. Clinical parameters such as gingival index were recorded. Samples were 
      cultured in routine aerobic and anaerobic medium. Bacterial growths were assessed 
      by semiquantitative methods. Bacterial isolates were confirmed by MALDI-TOF MS 
      technique. RESULTS: MALDI-TOF MS detected all the culture-grown bacteria. In 
      primary dentition group, purple and yellow complex bacteria predominated. 
      Streptococcus spp. was the predominant bacteria (51%) followed by Escherichia 
      coli (19%) and Veillonella spp. (19%). In mixed dentition and permanent group 
      also, Streptococcus spp. was predominant (46%) followed by Veillonella spp. (24%) 
      and E. coli(19%). However, in both groups, orange complex bacteria (bridge 
      complex) such as Prevotella nigrescens and red complex bacteria (Porphyromonas 
      gingivalis, 3%) were seen. For majority of bacteria, the load increased with age. 
      CONCLUSIONS: The bacterial isolates showed a distinct age-specific colonization. 
      The use of advanced technique such as MALDI-TOF MS is helpful in the detection of 
      periodontal pathogens, and the effective oral health programs can be implemented 
      to minimize the risk of periodontal diseases.
CI  - Copyright: (c) 2020 Journal of Indian Society of Periodontology.
FAU - Srivastava, Amitabh
AU  - Srivastava A
AD  - Department of Periodontology and Implantology, Sardar Patel Post Graduate 
      Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India.
FAU - Saha, Sonali
AU  - Saha S
AD  - Department of Periodontology and Implantology, Sardar Patel Post Graduate 
      Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India.
FAU - Sahu, Chinmoy
AU  - Sahu C
AD  - Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical 
      Sciences, Lucknow, Uttar Pradesh, India.
LA  - eng
PT  - Journal Article
DEP - 20200127
PL  - India
TA  - J Indian Soc Periodontol
JT  - Journal of Indian Society of Periodontology
JID - 101499342
PMC - PMC7069104
OTO - NOTNLM
OT  - Bacteria
OT  - dentition
OT  - matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
OT  - periodontal disease
COIS- There are no conflicts of interest.
EDAT- 2020/03/20 06:00
MHDA- 2020/03/20 06:01
PMCR- 2020/03/01
CRDT- 2020/03/20 06:00
PHST- 2019/05/22 00:00 [received]
PHST- 2019/11/28 00:00 [revised]
PHST- 2019/12/02 00:00 [accepted]
PHST- 2020/03/20 06:00 [entrez]
PHST- 2020/03/20 06:00 [pubmed]
PHST- 2020/03/20 06:01 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - JISP-24-104 [pii]
AID - 10.4103/jisp.jisp_303_19 [doi]
PST - ppublish
SO  - J Indian Soc Periodontol. 2020 Mar-Apr;24(2):104-108. doi: 
      10.4103/jisp.jisp_303_19. Epub 2020 Jan 27.