PMID- 32191156
OWN - NLM
STAT- MEDLINE
DCOM- 20201029
LR  - 20201029
IS  - 1502-3850 (Electronic)
IS  - 0001-6357 (Linking)
VI  - 78
IP  - 7
DP  - 2020 Oct
TI  - The effect of electromagnetic fields on survival and proliferation rate of dental 
      pulp stem cells.
PG  - 494-500
LID - 10.1080/00016357.2020.1734655 [doi]
AB  - Aims: Extremely low-frequency electromagnetic fields (ELF-EMF) can affect 
      biological systems and alter some cell functions like proliferation rate. Dental 
      pulp tissue is known as a source of multipotent stromal stem cells (MSCs), which 
      can be obtained by a less invasive and more available process compared to bone 
      marrow-derived stem cells (BMSCs). This study aimed to consider the effect of 
      ELF-EMF on proliferation rates of human dental pulp stem cells (hDPSCs).Material 
      and methods: ELF-EMF was generated by a system including autotransformer, 
      multi-meter, solenoid coils, teslameter and its probe. The effect of ELF-EMF with 
      the intensity of 0.5 and 1 mT and 50 Hz on the proliferation rate of hDPSCs was 
      assessed in 20 and 40 min per day for 7 days. MTT assay and DAPI test were used 
      to determine the growth and proliferation of DPSCs.Results: Based on MTT, ELF-EMF 
      has maximum effect with the intensity of 1 mT for 20 min/day on the proliferation 
      of hDPSCs. The survival and proliferation rate in all exposure groups were 
      significantly higher than the control group. Based on the data obtained from MTT 
      and DAPI assay, the number of viable cells in the group exposed to 1 mT for 
      20 min/day was higher than other groups (p < .05).Conclusions: Regarding to the 
      results of this study, 0.5 and 1 mT ELF-EMF can enhance survival and 
      proliferation rates of hDPSCs.
FAU - Samiei, Mohammad
AU  - Samiei M
AD  - Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical 
      Sciences, Tabriz, Iran.
FAU - Aghazadeh, Zahra
AU  - Aghazadeh Z
AD  - Department of Oral Medicine, Faculty of Dentistry, Tabriz University of Medical 
      Sciences, Tabriz, Iran.
FAU - Abdolahinia, Elaheh Dalir
AU  - Abdolahinia ED
AD  - Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz 
      University of Medical Sciences, Tabriz, Iran.
FAU - Vahdati, Amin
AU  - Vahdati A
AD  - Dental Public Health Program, Community Oral Health Department, School of 
      Dentistry, Shahid Beheshti University of Medical Sciences, Tehran.
FAU - Daneshvar, Sabalan
AU  - Daneshvar S
AD  - Department of Electrical and Computer Engineering, Faculty of Electrical 
      Engineering, University of Tabriz, Tabriz, Iran.
FAU - Noghani, Atefe
AU  - Noghani A
AD  - Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical 
      Sciences, Tabriz, Iran.
LA  - eng
PT  - Journal Article
DEP - 20200319
PL  - Sweden
TA  - Acta Odontol Scand
JT  - Acta odontologica Scandinavica
JID - 0370344
SB  - IM
MH  - *Cell Proliferation
MH  - *Dental Pulp
MH  - *Electromagnetic Fields/adverse effects
MH  - Humans
MH  - Stem Cells
OTO - NOTNLM
OT  - Human dental pulp stem cells
OT  - extremely low-frequency electromagnetic fields
OT  - proliferation rate
EDAT- 2020/03/20 06:00
MHDA- 2020/10/30 06:00
CRDT- 2020/03/20 06:00
PHST- 2020/03/20 06:00 [pubmed]
PHST- 2020/10/30 06:00 [medline]
PHST- 2020/03/20 06:00 [entrez]
AID - 10.1080/00016357.2020.1734655 [doi]
PST - ppublish
SO  - Acta Odontol Scand. 2020 Oct;78(7):494-500. doi: 10.1080/00016357.2020.1734655. 
      Epub 2020 Mar 19.