PMID- 32191627
OWN - NLM
STAT- MEDLINE
DCOM- 20200921
LR  - 20210825
IS  - 1865-7125 (Electronic)
IS  - 0341-0382 (Linking)
VI  - 75
IP  - 3-4
DP  - 2020 Mar 26
TI  - 3,5-Dicaffeoyl-epi-quinic acid inhibits the PMA-stimulated activation and 
      expression of MMP-9 but not MMP-2 via downregulation of MAPK pathway.
PG  - 113-120
LID - 10.1515/znc-2019-0163 [doi]
AB  - Matrix metalloproteinases (MMPs), especially MMP-2 and MMP-9, are very important 
      gelatinases that are overexpressed during tumor metastasis. Up to date, several 
      MMP inhibitors have been developed from natural sources as well as organic 
      synthesis. In the present study, the MMP-2 and MMP-9 inhibitory effects of 
      3,5-dicaffeoyl-epi-quinic acid (DCEQA), a caffeoylquinic acid derivative isolated 
      from Atriplex gmelinii, were investigated in phorbol 12-myristate 13-acetate 
      (PMA)-treated human HT1080 fibrosarcoma cells. Gelatin zymography and 
      immunoblotting showed that DCEQA significantly inhibited the PMA-induced 
      activation and expression of MMP-9 but was not able to show any effect against 
      MMP-2. DCEQA treatment was also shown to upregulate the protein expression of 
      tissue inhibitor of MMP-1 along with decreased MMP-9 protein levels. Moreover, 
      the effect of DCEQA on phosphorylation of mitogen activated protein kinases 
      (MAPKs), analyzed by immunoblotting, indicated the DCEQA inhibited the MMP-9 by 
      downregulation of MAPK pathway. Collectively, current results suggested that 
      DCEQA is a potent MMP-9 inhibitor and can be utilized as lead compound for 
      treatment of pathological complications involving enhanced MMP activity such as 
      cancer metastasis.
FAU - Lee, Jung Im
AU  - Lee JI
AD  - Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, 
      Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea.
FAU - Kil, Jung-Ha
AU  - Kil JH
AD  - Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, 
      Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea.
FAU - Yu, Ga Hyun
AU  - Yu GH
AD  - Department of Food and Nutrition, College of Medical and Life Sciences, Silla 
      University, Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea.
FAU - Karadeniz, Fatih
AU  - Karadeniz F
AD  - Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, 
      Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea.
FAU - Oh, Jung Hwan
AU  - Oh JH
AD  - Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, 
      Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea.
FAU - Seo, Youngwan
AU  - Seo Y
AD  - Division of Marine Bioscience, College of Ocean Science and Technology, Korea 
      Maritime and Ocean University, Busan 49112, Korea.
FAU - Kong, Chang-Suk
AU  - Kong CS
AUID- ORCID: 0000-0002-2304-9304
AD  - Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, 
      Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea.
AD  - Department of Food and Nutrition, College of Medical and Life Sciences, Silla 
      University, Baegyang-daero 700 beon-gil 140, Sasang-gu, Busan 46958, Korea, 
      Phone: +82-51-999-5429.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Z Naturforsch C J Biosci
JT  - Zeitschrift fur Naturforschung. C, Journal of biosciences
JID - 8912155
RN  - 0 (Phorbol Esters)
RN  - 0 (Plant Extracts)
RN  - 0 (caffeoylquinic acid)
RN  - 058C04BGYI (Quinic Acid)
RN  - 20839-06-9 (phorbol-12-myristate)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Atriplex/*chemistry
MH  - Cell Line, Tumor
MH  - Down-Regulation
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - MAP Kinase Signaling System/drug effects
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Matrix Metalloproteinase 9/*genetics/*metabolism
MH  - Molecular Structure
MH  - Phorbol Esters/*adverse effects
MH  - Plant Extracts/chemistry
MH  - Quinic Acid/*analogs & derivatives/chemistry/pharmacology
OTO - NOTNLM
OT  - 3,5-dicaffeoyl-epi-quinic acid
OT  - HT1080
OT  - MAPK
OT  - MMP-9
EDAT- 2020/03/20 06:00
MHDA- 2020/09/22 06:00
CRDT- 2020/03/20 06:00
PHST- 2019/09/20 00:00 [received]
PHST- 2020/02/10 00:00 [accepted]
PHST- 2020/03/20 06:00 [pubmed]
PHST- 2020/09/22 06:00 [medline]
PHST- 2020/03/20 06:00 [entrez]
AID - znc-2019-0163 [pii]
AID - 10.1515/znc-2019-0163 [doi]
PST - ppublish
SO  - Z Naturforsch C J Biosci. 2020 Mar 26;75(3-4):113-120. doi: 
      10.1515/znc-2019-0163.