PMID- 32192194
OWN - NLM
STAT- MEDLINE
DCOM- 20210217
LR  - 20210217
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 12
IP  - 3
DP  - 2020 Mar 17
TI  - Coxsackievirus B3 Infection of Human Neural Progenitor Cells Results in Distinct 
      Expression Patterns of Innate Immune Genes.
LID - 10.3390/v12030325 [doi]
LID - 325
AB  - Coxsackievirus B3 (CVB3), a member of Picornaviridae family, is an important 
      human pathogen that causes a wide range of diseases, including myocarditis, 
      pancreatitis, and meningitis. Although CVB3 has been well demonstrated to target 
      murine neural progenitor cells (NPCs), gene expression profiles of CVB3-infected 
      human NPCs (hNPCs) has not been fully explored. To characterize the molecular 
      signatures and complexity of CVB3-mediated host cellular responses in hNPCs, we 
      performed QuantSeq 3' mRNA sequencing. Increased expression levels of viral RNA 
      sensors (RIG-I, MDA5) and interferon-stimulated genes, such as IFN-beta, IP-10, 
      ISG15, OAS1, OAS2, Mx2, were detected in response to CVB3 infection, while IFN-gamma 
      expression level was significantly downregulated in hNPCs. Consistent with the 
      gene expression profile, CVB3 infection led to enhanced secretion of inflammatory 
      cytokines and chemokines, such as interleukin-6 (IL-6), interleukin-8 (IL-8), and 
      monocyte chemoattractant protein-1 (MCP-1). Furthermore, we show that type I 
      interferon (IFN) treatment in hNPCs leads to significant attenuation of CVB3 RNA 
      copy numbers, whereas, type II IFN (IFN-gamma) treatment enhances CVB3 replication 
      and upregulates suppressor of cytokine signaling 1/3 (SOCS) expression levels. 
      Taken together, our results demonstrate the distinct molecular patterns of 
      cellular responses to CVB3 infection in hNPCs and the pro-viral function of IFN-gamma 
      via the modulation of SOCS expression.
FAU - Oh, Soo-Jin
AU  - Oh SJ
AUID- ORCID: 0000-0001-5423-0440
AD  - Department of Biomedical Sciences, BK21 PLUS program, College of Medicine, Korea 
      University Guro Hospital, Seoul 08308, Korea.
FAU - Gim, Jeong-An
AU  - Gim JA
AD  - Medical Science Research Center, College of Medicine, Korea University Guro 
      Hospital, Seoul 08308, Korea.
FAU - Lee, Jae Kyung
AU  - Lee JK
AUID- ORCID: 0000-0002-9641-8473
AD  - Department of Biomedical Sciences, BK21 PLUS program, College of Medicine, Korea 
      University Guro Hospital, Seoul 08308, Korea.
FAU - Park, Hosun
AU  - Park H
AD  - Department of Microbiology, College of Medicine, Yeungnam University, 170 
      Hyeonchung-ro, Namgu, Daegu 42415, Korea.
FAU - Shin, Ok Sarah
AU  - Shin OS
AD  - Department of Biomedical Sciences, BK21 PLUS program, College of Medicine, Korea 
      University Guro Hospital, Seoul 08308, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200317
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Cytokines)
SB  - IM
MH  - Cell Line
MH  - Computational Biology/methods
MH  - Cytokines/metabolism
MH  - *Disease Susceptibility
MH  - Enterovirus B, Human/*physiology
MH  - Enterovirus Infections/genetics/virology
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - *Host-Pathogen Interactions/genetics/immunology
MH  - Humans
MH  - Immunity, Innate/*genetics
MH  - Neural Stem Cells/*metabolism/*virology
MH  - Signal Transduction
MH  - Transcriptome
PMC - PMC7150933
OTO - NOTNLM
OT  - Coxsackievirus B3 (CVB3)
OT  - gene expression profiles
OT  - interferons
OT  - neuronal progenitor cells
OT  - suppressor of cytokine signaling
COIS- The authors declare no conflict of interest.
EDAT- 2020/03/21 06:00
MHDA- 2021/02/18 06:00
PMCR- 2020/03/01
CRDT- 2020/03/21 06:00
PHST- 2020/02/13 00:00 [received]
PHST- 2020/03/09 00:00 [revised]
PHST- 2020/03/11 00:00 [accepted]
PHST- 2020/03/21 06:00 [entrez]
PHST- 2020/03/21 06:00 [pubmed]
PHST- 2021/02/18 06:00 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - v12030325 [pii]
AID - viruses-12-00325 [pii]
AID - 10.3390/v12030325 [doi]
PST - epublish
SO  - Viruses. 2020 Mar 17;12(3):325. doi: 10.3390/v12030325.