PMID- 3219265
OWN - NLM
STAT- MEDLINE
DCOM- 19890316
LR  - 20190515
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 58
IP  - 5
DP  - 1988 Nov
TI  - Enzymes of mannose metabolism in murine and human lymphocytic leukaemia.
PG  - 567-9
AB  - Mannose in animal cells is phosphorylated by hexokinase (HK) and later isomerised 
      by mannose phosphate isomerase (MPI) to fructose-6-P, which is incorporated in 
      the glycolysis pathway. In this paper we report a significant decrease of MPI 
      activity in splenic lymphoid cells from AKR/J old mice with lymphocytic leukaemia 
      in comparison to that found in splenic lymphocytes from AKR/J non-leukaemic young 
      mice and BALB/c young and old control mice. However, HK with mannose as substrate 
      presents a normal activity in AKR/J leukaemic mice. This marked shortage of MPI 
      explains the in vitro mannose toxicity found by us here in splenic lymphoid cells 
      from AKR/J leukaemic mice. MPI activity was also decreased in peripheral blood 
      lymphocytes from 4 out of the 6 patients studied with chronic lymphocytic 
      leukaemia in relation to the activity found in the lymphocytes from healthy 
      donors. The utility of analysing MPI activity in leukaemia patients and the use 
      of mannose as an innocuous chemotherapic supporting agent in patients with 
      decreased MPI activity is proposed.
FAU - de la Fuente, M
AU  - de la Fuente M
AD  - Department of Animal Biology II, Faculty of Biological Sciences, Complutense 
      University, Madrid, Spain.
FAU - Hernanz, A
AU  - Hernanz A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.1.3.- (Carbohydrate Epimerases)
RN  - EC 5.3.1.8 (Mannose-6-Phosphate Isomerase)
RN  - IY9XDZ35W2 (Glucose)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Animals
MH  - Carbohydrate Epimerases/*metabolism
MH  - Cell Survival/drug effects
MH  - Female
MH  - Glucose/metabolism
MH  - Hexokinase/*metabolism
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/enzymology
MH  - Leukemia, Lymphoid/*enzymology
MH  - Lymphocytes/enzymology
MH  - Male
MH  - Mannose/metabolism/pharmacology
MH  - Mannose-6-Phosphate Isomerase/*metabolism
MH  - Mice
MH  - Mice, Inbred AKR
MH  - Mice, Inbred BALB C
PMC - PMC2246808
EDAT- 1988/11/01 00:00
MHDA- 1988/11/01 00:01
CRDT- 1988/11/01 00:00
PHST- 1988/11/01 00:00 [pubmed]
PHST- 1988/11/01 00:01 [medline]
PHST- 1988/11/01 00:00 [entrez]
AID - 10.1038/bjc.1988.260 [doi]
PST - ppublish
SO  - Br J Cancer. 1988 Nov;58(5):567-9. doi: 10.1038/bjc.1988.260.