PMID- 32193924
OWN - NLM
STAT- MEDLINE
DCOM- 20210209
LR  - 20210209
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 92
IP  - 8
DP  - 2020 Apr 21
TI  - Metal Probe Microextraction Coupled to Dielectric Barrier Discharge 
      Ionization-Mass Spectrometry for Detecting Drug Residues in Organisms.
PG  - 5921-5928
LID - 10.1021/acs.analchem.0c00004 [doi]
AB  - In this study, a novel method for the direct coupling of metal probe 
      microextraction (MPME) and a dielectric barrier discharge ionization (DBDI) 
      source with mass spectrometry (MS) is reported. Analytes adsorbed on a tungsten 
      needle were directly transferred to the DBDI source via rapid thermal desorption, 
      which resulted in a limit of detection as low as 8 pg/mL. This is in part due to 
      the "active capillary" configuration of the plasma ion source, where the 
      efficiency of ion transfer to the MS is  approximately 100%. Specialty gases to maintain the 
      plasma and carry analytes to the MS are not required. In contrast to direct 
      one-step ionization of molecular adsorbates, the complete separation of the 
      analyte desorption from the probe and the ionization event in our experimental 
      setup greatly enhanced the sensitivity and detection reproducibility (RSD of 
      8.3%). We show detection of pyrimethamine, a first-line drug for the treatment 
      and prevention of Plasmodium falciparum malaria all over the world, by this 
      MPME/DBDI/MS method. The detection of drug residues in live fish and paramecium 
      was achieved without the need for any sample pretreatment. The relative 
      concentration of the drug in different organs of the fish was determined. This 
      simple and convenient method has the potential for the analysis of chemicals even 
      in single-cell organisms.
FAU - Lu, Qiao
AU  - Lu Q
AUID- ORCID: 0000-0001-6071-8477
AD  - Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis & 
      Instrumentation, College of Chemistry and Chemical Engineering, Xiamen 
      University, Xiamen 361005, China.
FAU - Lin, Rongkun
AU  - Lin R
AD  - Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis & 
      Instrumentation, College of Chemistry and Chemical Engineering, Xiamen 
      University, Xiamen 361005, China.
FAU - Du, Chao
AU  - Du C
AD  - Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis & 
      Instrumentation, College of Chemistry and Chemical Engineering, Xiamen 
      University, Xiamen 361005, China.
FAU - Meng, Yifan
AU  - Meng Y
AD  - Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis & 
      Instrumentation, College of Chemistry and Chemical Engineering, Xiamen 
      University, Xiamen 361005, China.
FAU - Yang, Manqing
AU  - Yang M
AD  - Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis & 
      Instrumentation, College of Chemistry and Chemical Engineering, Xiamen 
      University, Xiamen 361005, China.
FAU - Zenobi, Renato
AU  - Zenobi R
AUID- ORCID: 0000-0001-5211-4358
AD  - Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis & 
      Instrumentation, College of Chemistry and Chemical Engineering, Xiamen 
      University, Xiamen 361005, China.
AD  - ETH Zurich, Department of Chemistry and Applied Biosciences, 8093 Zurich, 
      Switzerland.
FAU - Hang, Wei
AU  - Hang W
AUID- ORCID: 0000-0002-9145-3181
AD  - Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis & 
      Instrumentation, College of Chemistry and Chemical Engineering, Xiamen 
      University, Xiamen 361005, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200331
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - V9306CXO6G (Tungsten)
RN  - Z3614QOX8W (Pyrimethamine)
SB  - IM
MH  - Animals
MH  - Cyprinidae
MH  - Drug Residues/*analysis
MH  - Paramecium
MH  - Pyrimethamine/*analysis
MH  - *Solid Phase Microextraction
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tungsten/*chemistry
EDAT- 2020/03/21 06:00
MHDA- 2021/02/10 06:00
CRDT- 2020/03/21 06:00
PHST- 2020/03/21 06:00 [pubmed]
PHST- 2021/02/10 06:00 [medline]
PHST- 2020/03/21 06:00 [entrez]
AID - 10.1021/acs.analchem.0c00004 [doi]
PST - ppublish
SO  - Anal Chem. 2020 Apr 21;92(8):5921-5928. doi: 10.1021/acs.analchem.0c00004. Epub 
      2020 Mar 31.