PMID- 32194220
OWN - NLM
STAT- MEDLINE
DCOM- 20201028
LR  - 20201028
IS  - 1872-8227 (Electronic)
IS  - 0168-8227 (Linking)
VI  - 167
DP  - 2020 Sep
TI  - miR-221-3p and miR-222-3p downregulation promoted osteogenic differentiation of 
      bone marrow mesenchyme stem cells through IGF-1/ERK pathway under high glucose 
      condition.
PG  - 108121
LID - S0168-8227(20)30371-5 [pii]
LID - 10.1016/j.diabres.2020.108121 [doi]
AB  - OBJECTIVE: This study aims to investigate the roles of miR-221-3p and miR-222-3p 
      in the regulation of osteogenic differentiation of bone marrow mesenchyme stem 
      cells (BMSCs) under high glucose condition. MATERIALS AND METHODS: The 
      expreesions of miR-221-3p, miR-222-3p and insulin-like growth factor 1 (IGF-1) 
      were detected by qRT-PCR. The protein levels of osteoblast-related proteins 
      (Osterix, Runx-2 and Osteopontin) were detected by western blot. Whether 
      miR-221-3p and miR-222-3p can target IGF-1 was assessed by dual luciferase 
      reporter gene assay. RESULTS: miR-221-3p and miR-222-3p were up-regulated in the 
      mandibles of diabetic rats and BMSCs cultured in high glucose condition. 
      Silencing miR-221-3p or/ and miR-222-3p increased ALP activity and up-regulated 
      osteoblast-related protein levels, and the simultaneous silence the two miRNAs 
      showed stronger effects on ALP activity and osteoblast-related protein levels. 
      Next, we confirmed that miR-221-3p and miR-222-3p both targeted IGF-1 and 
      cooperatively regulated its expression. Besides, miR-221-3p and miR-222-3p 
      regulated ERK activation through IGF-1. Silencing miR-221-3p and miR-222-3p 
      promoted osteogenic differentiation of BMSCs through IGF-1 under high glucose 
      condition. CONCLUSION: miR-221-3p and miR-222-3p inhibited osteogenic 
      differentiation of BMSCs via IGF-1/ERK pathway under high glucose condition.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Gan, Kang
AU  - Gan K
AD  - Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, People's Republic of China.
FAU - Dong, Guan-Hua
AU  - Dong GH
AD  - Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, People's Republic of China.
FAU - Wang, Ning
AU  - Wang N
AD  - Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, People's Republic of China.
FAU - Zhu, Juan-Fang
AU  - Zhu JF
AD  - Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, People's Republic of China. Electronic address: 
      zdzhujf@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200316
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (MicroRNAs)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Cell Differentiation/*genetics
MH  - Diabetes Mellitus, Experimental/metabolism
MH  - Down-Regulation/genetics
MH  - Glucose/genetics/*metabolism
MH  - Insulin-Like Growth Factor I/*genetics/metabolism
MH  - MAP Kinase Signaling System/genetics
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - MicroRNAs/*genetics/metabolism
MH  - Osteoblasts/metabolism
MH  - Osteogenesis/*genetics
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
OTO - NOTNLM
OT  - Bone marrow mesenchyme stem cells
OT  - IGF-1
OT  - Osteogenic differentiation
OT  - miR-221-3p
OT  - miR-222-3p
COIS- Declaration of Competing Interest The authors declared that there is no conflict 
      of interest.
EDAT- 2020/03/21 06:00
MHDA- 2020/10/29 06:00
CRDT- 2020/03/21 06:00
PHST- 2019/11/21 00:00 [received]
PHST- 2020/02/28 00:00 [revised]
PHST- 2020/03/11 00:00 [accepted]
PHST- 2020/03/21 06:00 [pubmed]
PHST- 2020/10/29 06:00 [medline]
PHST- 2020/03/21 06:00 [entrez]
AID - S0168-8227(20)30371-5 [pii]
AID - 10.1016/j.diabres.2020.108121 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 2020 Sep;167:108121. doi: 10.1016/j.diabres.2020.108121. 
      Epub 2020 Mar 16.