PMID- 32195350
OWN - NLM
STAT- MEDLINE
DCOM- 20201112
LR  - 20210919
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 6
IP  - 11
DP  - 2020 Mar
TI  - Platelet P-selectin initiates cross-presentation and dendritic cell 
      differentiation in blood monocytes.
PG  - eaaz1580
LID - 10.1126/sciadv.aaz1580 [doi]
LID - eaaz1580
AB  - Dendritic cells (DCs) are adept at cross-presentation and initiation of 
      antigen-specific immunity. Clinically, however, DCs produced by in vitro 
      differentiation of monocytes in the presence of exogenous cytokines have been met 
      with limited success. We hypothesized that DCs produced in a physiological manner 
      may be more effective and found that platelets activate a cross-presentation 
      program in peripheral blood monocytes with rapid (18 hours) maturation into 
      physiological DCs (phDCs). Differentiation of monocytes into phDCs was 
      concomitant with the formation of an "adhesion synapse," a biophysical junction 
      enriched with platelet P-selectin and monocyte P-selectin glycoprotein ligand 1, 
      followed by intracellular calcium fluxing and nuclear localization of nuclear 
      factor kappaB. phDCs were more efficient than cytokine-derived DCs in generating 
      tumor-specific T cell immunity. Our findings demonstrate that platelets mediate a 
      cytokine-independent, physiologic maturation of DC and suggest a novel strategy 
      for DC-based immunotherapies.
CI  - Copyright (c) 2020 The Authors, some rights reserved; exclusive licensee American 
      Association for the Advancement of Science. No claim to original U.S. Government 
      Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 
      (CC BY-NC).
FAU - Han, Patrick
AU  - Han P
AUID- ORCID: 0000-0002-0370-0729
AD  - Department of Chemical and Environmental Engineering, School of Engineering and 
      Applied Science, Yale University, New Haven, CT 06511, USA.
FAU - Hanlon, Douglas
AU  - Hanlon D
AUID- ORCID: 0000-0003-4386-7491
AD  - Department of Dermatology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
FAU - Arshad, Najla
AU  - Arshad N
AUID- ORCID: 0000-0001-5553-311X
AD  - Department of Immunobiology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
FAU - Lee, Jung Seok
AU  - Lee JS
AD  - Department of Biomedical Engineering, School of Engineering and Applied Science, 
      Yale University, New Haven, CT 06511, USA.
FAU - Tatsuno, Kazuki
AU  - Tatsuno K
AD  - Department of Dermatology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
FAU - Robinson, Eve
AU  - Robinson E
AD  - Department of Dermatology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
FAU - Filler, Renata
AU  - Filler R
AD  - Department of Dermatology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
FAU - Sobolev, Olga
AU  - Sobolev O
AD  - Department of Dermatology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
FAU - Cote, Christine
AU  - Cote C
AD  - Yale Flow Cytometry Facility, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
FAU - Rivera-Molina, Felix
AU  - Rivera-Molina F
AD  - Yale CINEMA Lab, School of Medicine, Yale University, New Haven, CT 06511, USA.
FAU - Toomre, Derek
AU  - Toomre D
AD  - Yale CINEMA Lab, School of Medicine, Yale University, New Haven, CT 06511, USA.
FAU - Fahmy, Tarek
AU  - Fahmy T
AUID- ORCID: 0000-0001-8740-4179
AD  - Department of Chemical and Environmental Engineering, School of Engineering and 
      Applied Science, Yale University, New Haven, CT 06511, USA.
AD  - Department of Dermatology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
AD  - Department of Immunobiology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
AD  - Department of Biomedical Engineering, School of Engineering and Applied Science, 
      Yale University, New Haven, CT 06511, USA.
FAU - Edelson, Richard
AU  - Edelson R
AUID- ORCID: 0000-0002-1552-2480
AD  - Department of Dermatology, School of Medicine, Yale University, New Haven, CT 
      06511, USA.
LA  - eng
GR  - R01 AI097206/AI/NIAID NIH HHS/United States
GR  - UL1 TR001863/TR/NCATS NIH HHS/United States
GR  - T32 AR007016/AR/NIAMS NIH HHS/United States
GR  - R01 GM118486/GM/NIGMS NIH HHS/United States
GR  - P30 DK045735/DK/NIDDK NIH HHS/United States
GR  - R21 AG026412/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200311
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (P-Selectin)
RN  - 0 (P-selectin ligand protein)
SB  - IM
EIN - Sci Adv. 2021 Aug 27;7(35):. PMID: 34452922
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens, Neoplasm/genetics/immunology
MH  - Blood Platelets/*immunology
MH  - Calcium Signaling/genetics/*immunology
MH  - Cell Differentiation/genetics/*immunology
MH  - Dendritic Cells/*immunology
MH  - Membrane Glycoproteins/genetics/immunology
MH  - Mice
MH  - Mice, Transgenic
MH  - Monocytes/*immunology
MH  - NF-kappa B/genetics/immunology
MH  - P-Selectin/genetics/*immunology
MH  - T-Lymphocytes/immunology
PMC - PMC7065880
EDAT- 2020/03/21 06:00
MHDA- 2020/11/13 06:00
PMCR- 2020/03/11
CRDT- 2020/03/21 06:00
PHST- 2019/08/16 00:00 [received]
PHST- 2019/12/17 00:00 [accepted]
PHST- 2020/03/21 06:00 [entrez]
PHST- 2020/03/21 06:00 [pubmed]
PHST- 2020/11/13 06:00 [medline]
PHST- 2020/03/11 00:00 [pmc-release]
AID - aaz1580 [pii]
AID - 10.1126/sciadv.aaz1580 [doi]
PST - epublish
SO  - Sci Adv. 2020 Mar 11;6(11):eaaz1580. doi: 10.1126/sciadv.aaz1580. eCollection 
      2020 Mar.