PMID- 32196930
OWN - NLM
STAT- MEDLINE
DCOM- 20211112
LR  - 20211112
IS  - 1521-6551 (Electronic)
IS  - 1521-6543 (Linking)
VI  - 72
IP  - 7
DP  - 2020 Jul
TI  - Lack of miR-1246 in small extracellular vesicle blunts tumorigenesis of laryngeal 
      carcinoma cells by regulating Cyclin G2.
PG  - 1491-1503
LID - 10.1002/iub.2274 [doi]
AB  - Small extracellular vesicle (sEV) has precise impacts on tumor microenvironment 
      and play vital functions in intercellular interaction. However, the functional 
      role of sEV miRNA on laryngeal squamous cell carcinoma (LSCC) is largely 
      unresolved. Here, the expression of miR-1246 in LSCC tissues and plasma sEV was 
      examined. The internalization ability of sEV was determined by uptake assay. 
      Then, the source and purity of sEV were checked through RNase and/or 
      pharmacological inhibitors application. The invasion, migration, proliferation, 
      and cell cycle assays were used to determine the altered abilities of miR-1246 in 
      sEV in LSCC. Finally, target gene of miR-1246, Cyclin G2 (CCNG2), was stained 
      immunohistochemically. In addition, the relationship between CCNG2 and 
      clinicopathological features of patients was analyzed. We found that miR-1246 was 
      higher in LSCC tissues and plasma sEV. MiR-1246 was enriched in sEV rather than 
      soluble form. SEV could be internalized into adjacent cells. Lack of miR-1246 in 
      sEV abrogated the tumorigenesis of LSCC. Furthermore, CCNG2 knockdown arrested 
      the cell cycle and correlated to clinicopathological features and prognosis of 
      LSCC patients. Taken together, we found that the function of sEV miR-1246 by 
      regulating CCNG2 is responsible for LSCC advancement with emphasis on the main 
      source of miR-1246 mainly root in sEV rather than in soluble form.
CI  - (c) 2020 International Union of Biochemistry and Molecular Biology.
FAU - Huang, Qiang
AU  - Huang Q
AUID- ORCID: 0000-0002-5455-9454
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Hsueh, Chi-Yao
AU  - Hsueh CY
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Guo, Yang
AU  - Guo Y
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Wu, Xiu-Fa
AU  - Wu XF
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Li, Jiao-Yu
AU  - Li JY
AD  - Department of Pediatric, Xinhua Hospital, Shanghai Jiaotong University School of 
      Medicine, Shanghai, China.
FAU - Zhou, Liang
AU  - Zhou L
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200320
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cyclin G2)
RN  - 0 (MIRN1246 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Aged
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Carcinogenesis/genetics/metabolism/*pathology
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Cycle
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cyclin G2/genetics/*metabolism
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness
MH  - Prognosis
MH  - Survival Rate
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - CCNG2
OT  - head and neck cancer
OT  - laryngeal carcinoma
OT  - miR-1246
OT  - sEV
EDAT- 2020/03/21 06:00
MHDA- 2021/11/16 06:00
CRDT- 2020/03/21 06:00
PHST- 2020/02/03 00:00 [received]
PHST- 2020/02/27 00:00 [revised]
PHST- 2020/03/03 00:00 [accepted]
PHST- 2020/03/21 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2020/03/21 06:00 [entrez]
AID - 10.1002/iub.2274 [doi]
PST - ppublish
SO  - IUBMB Life. 2020 Jul;72(7):1491-1503. doi: 10.1002/iub.2274. Epub 2020 Mar 20.