PMID- 32197417
OWN - NLM
STAT- MEDLINE
DCOM- 20201201
LR  - 20201201
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 6
DP  - 2020 Mar 18
TI  - Ellagic Acid and Urolithins A and B Differentially Regulate Fat Accumulation and 
      Inflammation in 3T3-L1 Adipocytes While Not Affecting Adipogenesis and Insulin 
      Sensitivity.
LID - 10.3390/ijms21062086 [doi]
LID - 2086
AB  - Ellagic acid (EA) is a component of ellagitannins, present in crops such as 
      pecans, walnuts, and many berries, which metabolized by the gut microbiota forms 
      urolithins A, B, C, or D. In this study, ellagic acid, as well as urolithins A 
      and B, were tested on 3T3-L1 preadipocytes for differentiation and lipid 
      accumulation. In addition, inflammation was studied in mature adipocytes 
      challenged with lipopolysaccharide (LPS). Results indicated that EA and 
      urolithins A and B did not affect differentiation (adipogenesis) and only EA and 
      urolithin A attenuated lipid accumulation (lipogenesis), which seemed to be 
      through gene regulation of glucose transporter type 4 (GLUT4) and adiponectin. On 
      the other hand, gene expression of cytokines and proteins associated with the 
      inflammation process indicate that urolithins and EA differentially inhibit tumor 
      necrosis factor alpha (TNFalpha), inducible nitric oxide synthase (iNOS), interleukin 
      6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1). Urolithins A and B were 
      found to reduce nuclear levels of phosphorylated nuclear factor kappaB (p-NF-kappaB), 
      whereas all treatments showed expression of nuclear phosphorylated protein kinase 
      B (p-AKT) in challenged LPS cells when treated with insulin, indicating the fact 
      that adipocytes remained insulin sensitive. In general, urolithin A is a compound 
      able to reduce lipid accumulation, without affecting the protein expression of 
      peroxisome proliferator-activated receptor-gamma (PPARgamma), CCAAT/enhancer binding 
      protein-alpha (c/EBPalpha), and PPARalpha, whereas EA and urolithin B were found to enhance 
      PPARgamma and c/EBPalpha protein expressions as well as fatty acid (FA) oxidation, and 
      differentially affected lipid accumulation.
FAU - Cisneros-Zevallos, Luis
AU  - Cisneros-Zevallos L
AD  - Department of Horticultural Sciences, Texas A&M University, College Station, TX 
      77843-2133, USA.
AD  - Department of Nutrition and Food Science, Texas A&M University, College Station, 
      TX 77843, USA.
FAU - Bang, Woo Young
AU  - Bang WY
AD  - Department of Horticultural Sciences, Texas A&M University, College Station, TX 
      77843-2133, USA.
FAU - Delgadillo-Puga, Claudia
AU  - Delgadillo-Puga C
AD  - Departamento de Nutricion Animal Dr. Fernando Perez-Gil Romo, Instituto Nacional 
      de Ciencias Medicas y Nutricion Salvador Zubiran (INCMNSZ), CDMX 14080, Mexico.
LA  - eng
PT  - Journal Article
DEP - 20200318
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Coumarins)
RN  - 0 (urolithin B)
RN  - 1143-70-0 (3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one)
RN  - 19YRN3ZS9P (Ellagic Acid)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*metabolism
MH  - Adipogenesis/*drug effects
MH  - Animals
MH  - Coumarins/*pharmacology
MH  - Ellagic Acid/*pharmacology
MH  - *Insulin Resistance
MH  - Lipogenesis/*drug effects
MH  - Mice
PMC - PMC7139477
OTO - NOTNLM
OT  - 3T3-L1 adipocytes
OT  - adipogenesis
OT  - ellagic acid
OT  - inflammation
OT  - insulin sensitivity
OT  - lipid metabolism
OT  - urolithins A and B
COIS- The authors declare no conflict of interest.
EDAT- 2020/03/22 06:00
MHDA- 2020/12/02 06:00
PMCR- 2020/03/01
CRDT- 2020/03/22 06:00
PHST- 2019/12/14 00:00 [received]
PHST- 2020/03/10 00:00 [revised]
PHST- 2020/03/11 00:00 [accepted]
PHST- 2020/03/22 06:00 [entrez]
PHST- 2020/03/22 06:00 [pubmed]
PHST- 2020/12/02 06:00 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - ijms21062086 [pii]
AID - ijms-21-02086 [pii]
AID - 10.3390/ijms21062086 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Mar 18;21(6):2086. doi: 10.3390/ijms21062086.