PMID- 32198702
OWN - NLM
STAT- MEDLINE
DCOM- 20210719
LR  - 20210719
IS  - 1995-8218 (Electronic)
IS  - 1673-7067 (Print)
IS  - 1995-8218 (Linking)
VI  - 36
IP  - 7
DP  - 2020 Jul
TI  - Overexpression of Purinergic P2X4 Receptors in Hippocampus Rescues Memory 
      Impairment in Rats with Type 2 Diabetes.
PG  - 719-732
LID - 10.1007/s12264-020-00478-7 [doi]
AB  - Purinergic receptors have been reported to be involved in brain disorders. In 
      this study, we explored their roles and mechanisms underlying the memory 
      impairment in rats with type 2 diabetes mellitus (T2DM). T2DM rats exhibited a 
      worse performance in the T-maze and Morris water maze (MWM) than controls. 
      Microglia positive for P2X purinoceptor 4 (P2X4R) in the hippocampus were reduced 
      and activated microglia were increased in T2DM rats. Long Amplicon PCR (LA-PCR) 
      showed that DNA amplification of the p2x4r gene in the hippocampus was lower in 
      T2DM rats. Minocycline significantly reduced the number of activated microglia 
      and the mean distance traveled by T2DM rats in the MWM. Most importantly, P2X4R 
      overexpression suppressed the activated microglia and rescued the memory 
      impairment of T2DM rats. Overall, T2DM led to excessive activation of microglia 
      in the hippocampus, partly through the DNA damage-mediated downregulation of 
      P2X4Rs, thus contributing to memory impairment.
FAU - Zhang, Ping-An
AU  - Zhang PA
AD  - Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of 
      Neuroscience, Soochow University, Suzhou, 215123, China.
AD  - Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of 
      Soochow University, Suzhou, 215600, China.
FAU - Sun, Qian
AU  - Sun Q
AD  - Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of 
      Neuroscience, Soochow University, Suzhou, 215123, China.
FAU - Li, Yong-Chang
AU  - Li YC
AD  - Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of 
      Neuroscience, Soochow University, Suzhou, 215123, China.
FAU - Weng, Rui-Xia
AU  - Weng RX
AD  - The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
FAU - Wu, Rui
AU  - Wu R
AD  - Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of 
      Neuroscience, Soochow University, Suzhou, 215123, China.
FAU - Zhang, Hong-Hong
AU  - Zhang HH
AD  - The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China.
FAU - Xu, Guang-Yin
AU  - Xu GY
AD  - Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of 
      Neuroscience, Soochow University, Suzhou, 215123, China. guangyinxu@suda.edu.cn.
AD  - Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of 
      Soochow University, Suzhou, 215600, China. guangyinxu@suda.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200320
PL  - Singapore
TA  - Neurosci Bull
JT  - Neuroscience bulletin
JID - 101256850
RN  - 0 (Receptors, Purinergic P2X4)
SB  - IM
MH  - Animals
MH  - Cognition Disorders/etiology/*genetics
MH  - *Diabetes Mellitus, Type 2/complications
MH  - Hippocampus
MH  - Male
MH  - Microglia
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Receptors, Purinergic P2X4/genetics
PMC - PMC7340685
OTO - NOTNLM
OT  - DNA damage
OT  - Memory impairment
OT  - Microglia
OT  - P2X4 receptors
OT  - Type 2 diabetes mellitus
COIS- No conflicts of interest, financial or otherwise, are declared by the authors.
EDAT- 2020/03/22 06:00
MHDA- 2021/07/20 06:00
PMCR- 2020/03/20
CRDT- 2020/03/22 06:00
PHST- 2019/08/23 00:00 [received]
PHST- 2019/11/25 00:00 [accepted]
PHST- 2020/03/22 06:00 [pubmed]
PHST- 2021/07/20 06:00 [medline]
PHST- 2020/03/22 06:00 [entrez]
PHST- 2020/03/20 00:00 [pmc-release]
AID - 10.1007/s12264-020-00478-7 [pii]
AID - 478 [pii]
AID - 10.1007/s12264-020-00478-7 [doi]
PST - ppublish
SO  - Neurosci Bull. 2020 Jul;36(7):719-732. doi: 10.1007/s12264-020-00478-7. Epub 2020 
      Mar 20.