PMID- 32199138
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20200622
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 222
DP  - 2020 May
TI  - The effects of chronic cadmium exposure on Bufo gargarizans larvae: 
      Histopathological impairment, gene expression alteration and fatty acid 
      metabolism disorder in the liver.
PG  - 105470
LID - S0166-445X(19)30887-2 [pii]
LID - 10.1016/j.aquatox.2020.105470 [doi]
AB  - Cadmium (Cd) a highly toxic metal to human and wildlife health and it is 
      hazardous to both terrestrial and aquatic life. In this study, we used RNA 
      sequencing analysis to examine the effects of chronic cadmium exposure on liver 
      lipid metabolism of Bufo gargarizans larvae. Tadpoles were exposed to cadmium 
      concentrations at 0, 5, 10, 50, 100 and 200 mug L(-1) from Gosner stage 26-42 of 
      metamorphic climax. The results showed high dose cadmium (50, 100 and 
      200 mug L(-1)) caused obvious histological changes characterized by hepatocytes 
      deformation, nuclear pyknosis, increasing melanomacrophage centers (MMCs) and 
      aggregated lipid droplets. Moreover, transcriptome analysis showed that liver 
      function was seriously affected by cadmium exposure. Furthermore, high dose 
      cadmium significantly upregulated the mRNA expression of elongation of 
      very-long-chain fatty acids 1 (ELOVL1), Mitochondrial trans-2-enoyl-CoA reductase 
      (MECR), Trans-2, 3-enoyl-CoA reductase (TER) and Hydroxysteroid (17beta) 
      dehydrogenase type 12 (HSD17B12) which are related with fatty acid synthesis. 
      Meanwhile, mRNA levels of genes related with fat acid oxidation such as 
      acetyl-CoA acyltransferase 2 (ACAA2) and enoyl-coenzyme A (CoA) hydratase short 
      chain 1 (ECHS1) were significantly upregulated while the expression of Acyl-coA 
      thioesterase 1 (ACOT1), 3-hydroxyacyl-CoA dehydrogenase (HADH), Palmitoyl-protein 
      thioesterase 1(PPT1) and Acetyl-CoA acyltransferase 1(ACAA1) was significantly 
      downregulated by high dose cadmium exposure. Furthermore, the mRNA level of 
      ATP-binding cassette subfamily B member 11 (ABCB11) related with bile secretion 
      was significantly decreased exposed to high dose cadmium. Our results suggested 
      cadmium can cause liver dysfunction by inducing histopathological damages, 
      genetic expression alterations and fatty acid metabolism disorder.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Ju, Zongqi
AU  - Ju Z
AD  - College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China.
FAU - Ya, Jing
AU  - Ya J
AD  - College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China.
FAU - Li, Xinyi
AU  - Li X
AD  - College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China.
FAU - Wang, Hongyuan
AU  - Wang H
AD  - College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China.
FAU - Zhao, Hongfeng
AU  - Zhao H
AD  - College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China. 
      Electronic address: zhaohf@snnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200318
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - 00BH33GNGH (Cadmium)
RN  - EC 4.2.1.17 (Enoyl-CoA Hydratase)
SB  - IM
MH  - Animals
MH  - Bufonidae
MH  - Cadmium/*toxicity
MH  - Enoyl-CoA Hydratase/metabolism
MH  - Fatty Acids/*metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Larva/*drug effects/genetics/metabolism
MH  - Lipid Metabolism/drug effects/genetics
MH  - Liver/*drug effects/metabolism
MH  - Oxidation-Reduction
MH  - RNA, Messenger/metabolism
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - Fatty acid metabolism
OT  - Histopathology
OT  - Liver
OT  - Tadpole
OT  - Transcriptome
COIS- Declaration of Competing Interest The authors declared no competing interests.
EDAT- 2020/03/22 06:00
MHDA- 2020/06/23 06:00
CRDT- 2020/03/22 06:00
PHST- 2019/10/25 00:00 [received]
PHST- 2020/02/16 00:00 [revised]
PHST- 2020/03/10 00:00 [accepted]
PHST- 2020/03/22 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2020/03/22 06:00 [entrez]
AID - S0166-445X(19)30887-2 [pii]
AID - 10.1016/j.aquatox.2020.105470 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2020 May;222:105470. doi: 10.1016/j.aquatox.2020.105470. Epub 2020 
      Mar 18.