PMID- 32199923
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210626
IS  - 2531-1387 (Electronic)
IS  - 2531-1379 (Print)
IS  - 2531-1379 (Linking)
VI  - 43
IP  - 2
DP  - 2021 Apr-Jun
TI  - Use of the reticulocyte channel warmed to 41 degrees C of the XN-9000 analyzer in samples 
      with the presence of cold agglutinins.
PG  - 147-155
LID - S2531-1379(20)30024-9 [pii]
LID - 10.1016/j.htct.2020.01.004 [doi]
AB  - OBJECTIVES: The purpose of this study was to compare data obtained from the 
      reticulocyte channel (RET channel) heated to 41 degrees C with those obtained from 
      impedance channel (I-Channel) at room temperature in the samples with the mean 
      corpuscular hemoglobin concentration (MCHC)<370g/L and in samples with the 
      MCHC>370g/L, in the presence of cold agglutinins. METHODS: In this study, 60 
      blood samples (group 1) with the MCHC<370g/L (without cold agglutinins) and 78 
      blood samples (group 2) with the MCHC>370g/L (with cold agglutinins) were used to 
      compare the two analytical channels of the XN-9000 analyzer in different 
      preanalytical conditions. The parameters evaluated in both groups were the 
      following: red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean cell 
      volume (MCV), RBC-most frequent volume (R-MFV), mean hemoglobin concentration 
      (MCH) and mean cellular hemoglobin concentration (MCHC). RESULTS: The results of 
      this study showed an excellent correlation with both channels of the XN-9000 
      analyzer in samples with and without cold agglutinins, except for the MCHC. The 
      bias between the values obtained in the I-channel and those obtained in the RET 
      channel of both groups was insignificant and remained within the limits of 
      acceptability, as reported by Ricos et al. for all considered parameters, except 
      for MCHC. CONCLUSIONS: The presence of cold agglutinins in blood samples can be 
      detected by a spurious lowering of the RBC count and by a spurious increase in 
      the MCHC. The RET channel represents a great opportunity to correct the RBC count 
      in a rapid manner without preheating. However, neither methodology can completely 
      solve the residual presence of cold agglutinins in all samples, despite the MCHC 
      values being < 370g/L.
CI  - Copyright (c) 2020 Associacao Brasileira de Hematologia, Hemoterapia e Terapia 
      Celular. Published by Elsevier Espana, S.L.U. All rights reserved.
FAU - Roccaforte, Vincenzo
AU  - Roccaforte V
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy; Hospital Alessandro 
      Manzoni, Lecco, Italy; University of Study Milan, Milan, Italy. Electronic 
      address: v.roccaforte@asst-lecco.i.
FAU - Sciarini, Flavia
AU  - Sciarini F
AD  - Hospital Alessandro Manzoni, Lecco, Italy.
FAU - Proserpio, Vanessa
AU  - Proserpio V
AD  - Hospital Alessandro Manzoni, Lecco, Italy.
FAU - Buonocore, Ruggero
AU  - Buonocore R
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Zavaroni, Emanuela Marina
AU  - Zavaroni EM
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Burati, Silvia
AU  - Burati S
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Bussetti, Marco
AU  - Bussetti M
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Liuzzi, Giammaria
AU  - Liuzzi G
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Russo, Rosalba Monica
AU  - Russo RM
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Porreca, Wanda Patrizia
AU  - Porreca WP
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Angelis, Maria Luisa De
AU  - Angelis ML
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Perno, Carlo Federico
AU  - Perno CF
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
FAU - Bonato, Claudio
AU  - Bonato C
AD  - Hospital Alessandro Manzoni, Lecco, Italy.
FAU - Pastori, Stefano
AU  - Pastori S
AD  - ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200306
PL  - Brazil
TA  - Hematol Transfus Cell Ther
JT  - Hematology, transfusion and cell therapy
JID - 101725732
PMC - PMC8211624
OTO - NOTNLM
OT  - Autoimmune hemolytic anemia
OT  - Cold agglutinins
OT  - RET channel
OT  - Sysmex
OT  - XN-9000
EDAT- 2020/03/23 06:00
MHDA- 2020/03/23 06:01
PMCR- 2020/03/06
CRDT- 2020/03/23 06:00
PHST- 2019/09/09 00:00 [received]
PHST- 2019/11/25 00:00 [revised]
PHST- 2020/01/09 00:00 [accepted]
PHST- 2020/03/23 06:00 [pubmed]
PHST- 2020/03/23 06:01 [medline]
PHST- 2020/03/23 06:00 [entrez]
PHST- 2020/03/06 00:00 [pmc-release]
AID - S2531-1379(20)30024-9 [pii]
AID - 10.1016/j.htct.2020.01.004 [doi]
PST - ppublish
SO  - Hematol Transfus Cell Ther. 2021 Apr-Jun;43(2):147-155. doi: 
      10.1016/j.htct.2020.01.004. Epub 2020 Mar 6.