PMID- 32200917
OWN - NLM
STAT- MEDLINE
DCOM- 20201110
LR  - 20201110
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 213
DP  - 2020 Jun 1
TI  - A novel fluorescence probe of Plasmodium vivax lactate dehydrogenase based on 
      adenosine monophosphate protected bimetallic nanoclusters.
PG  - 120850
LID - S0039-9140(20)30141-7 [pii]
LID - 10.1016/j.talanta.2020.120850 [doi]
AB  - Specific detection of Plasmodium vivax lactate dehydrogenase (PvLDH), an 
      important biomarker of malaria, remains a significant challenge. Herein, 
      adenosine monophosphate protected gold-silver bimetallic nanoclusters, 
      Au-AgNCs@AMP were used as a specific and sensitive fluorescence probe to detect 
      PvLDH. After optimizing, a linear response was shown over a wide concentration 
      range (10-100 nM) and an extremely low limit of detection (LOD) at 0.10 nM 
      (3.7 ng mL(-1)) was achieved finally. Albeit the method was able to detect PvLDH 
      sensitively, it could not discriminate different types of LDHs. Consequently, 
      Al(3+) was employed as an "assistant agent", which induced an assay capacity to 
      discriminate PvLDH from other LDHs. The bimetallic nanoclusters inhibited the 
      activity of PvLDH, suggesting it bound near the active site of PvLDH with high 
      affinity. Zeta potential and UV-vis absorption experiments showed that 
      electrostatic interaction was the main driving force for the interaction between 
      the nanoclusters and PvLDH. Through chemical modification it indicated free thiol 
      groups in PvLDH played an implant role in the interaction. Overall, the 
      fluorescence enhancement and blue-shift were attributed to assembly-induced 
      emission enhancement (AIEE) and hydrophobic transfer. The present study provides 
      a simple, robust, and easy-to-perform approach to detect PvLDH with high 
      sensitivity and selectivity, with significant potential for malaria diagnosis in 
      the developing world.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhang, Chun-Xia
AU  - Zhang CX
AD  - State Key Laboratory of Supramolecular Structure and Materials, Institute of 
      Theoretical Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 
      130012, China.
FAU - Tanner, Julian A
AU  - Tanner JA
AD  - School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of 
      Hong Kong, Pokfulam, Hong Kong, China.
FAU - Li, Hong-Wei
AU  - Li HW
AD  - State Key Laboratory of Supramolecular Structure and Materials, Institute of 
      Theoretical Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 
      130012, China. Electronic address: lihongwei@jlu.edu.cn.
FAU - Wu, Yuqing
AU  - Wu Y
AD  - State Key Laboratory of Supramolecular Structure and Materials, Institute of 
      Theoretical Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 
      130012, China.
LA  - eng
PT  - Journal Article
DEP - 20200214
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Fluorescent Dyes)
RN  - 3M4G523W1G (Silver)
RN  - 415SHH325A (Adenosine Monophosphate)
RN  - 7440-57-5 (Gold)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Adenosine Monophosphate/*chemistry
MH  - Enzyme Assays/*methods
MH  - Fluorescent Dyes/*chemistry
MH  - Gold/chemistry
MH  - Humans
MH  - L-Lactate Dehydrogenase/*analysis
MH  - Limit of Detection
MH  - Malaria, Vivax/parasitology
MH  - Metal Nanoparticles/*chemistry
MH  - Plasmodium vivax/*enzymology
MH  - Silver/chemistry
OTO - NOTNLM
OT  - Assembly-induced emission enhancement
OT  - Bimetallic nanoclusters
OT  - Fluorescence enhancement
OT  - Inhibition
OT  - Plasmodium vivax lactate dehydrogenase
COIS- Declaration of competing interest There are no conflicts of interest to declare.
EDAT- 2020/03/24 06:00
MHDA- 2020/11/11 06:00
CRDT- 2020/03/24 06:00
PHST- 2019/10/22 00:00 [received]
PHST- 2020/02/10 00:00 [revised]
PHST- 2020/02/13 00:00 [accepted]
PHST- 2020/03/24 06:00 [entrez]
PHST- 2020/03/24 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
AID - S0039-9140(20)30141-7 [pii]
AID - 10.1016/j.talanta.2020.120850 [doi]
PST - ppublish
SO  - Talanta. 2020 Jun 1;213:120850. doi: 10.1016/j.talanta.2020.120850. Epub 2020 Feb 
      14.