PMID- 32201429
OWN - NLM
STAT- MEDLINE
DCOM- 20210104
LR  - 20210104
IS  - 1883-0498 (Electronic)
IS  - 0023-2513 (Print)
IS  - 0023-2513 (Linking)
VI  - 65
IP  - 4
DP  - 2020 Mar 9
TI  - Vitamin D Ameliorates Kidney Ischemia Reperfusion Injury via Reduction of 
      Inflammation and Myofibroblast Expansion.
PG  - E138-E143
AB  - The incidence rate of Acute Kidney Injury (AKI) gets escalated each year. Kidney 
      ischemia/reperfusion injury (IR injury) is the main cause of AKI after major 
      cardiovascular surgery, trauma, or kidney transplantation. Reperfusion is 
      considered essential for ischemic tissue. However, the evidence revealed that 
      reperfusion itself has impact in cellular destruction. Vitamin D is not only 
      known as calcium regulating hormone, but also as renoprotective agent. This study 
      aimed to investigate the effect of vitamin D treatment on kidney IR injury in 
      mice. Kidney IR injury was performed using 30 minutes of bilateral clamping of 
      renal pedicles, then released in male Swiss Webster mice (3 months, 30-40 grams, 
      n=20), which were divided into three groups: sham operation (SO) group, IR injury 
      (IRI) group, and IR injury with 0.25 microg/ kg body weight of vitamin D treatment 
      (IR7+VD). Mice were terminated at day 7 post operation, kidneys were harvested 
      and used for paraffin making, immunostaining and RNA extraction. Tubular injury 
      was quantified based on Periodic Acid-Schiff's (PAS) staining. Immunostaining was 
      done for quantification of macrophage (CD68) and myofibroblast (alpha-SMA). Reverse 
      Transcriptase PCR (RT-PCR) was done to examine Monocyte Chemoattractant Protein-1 
      (MCP-1) and Toll-like Receptor 4 (TLR4) mRNA expression. Kidney IR injury induced 
      significant increase of tubular injury, which was associated with higher 
      myofibroblast and macrophage number. Meanwhile, Vitamin D treatment significantly 
      reduced tubular, myofibroblast and macrophage number. RTPCR revealed reduction of 
      TLR4 and MCP-1 mRNA expressions after Vitamin D treatment (p<0.05 vs IR group). 
      Vitamin D ameliorates kidney IR injury through reducing inflammation and 
      myofibroblast formation.
FAU - Arfian, Nur
AU  - Arfian N
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Budiharjo, Santosa
AU  - Budiharjo S
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Wibisono, Dian Prasetyo
AU  - Wibisono DP
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Setyaningsih, Wiwit Ananda Wahyu
AU  - Setyaningsih WAW
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Romi, Muhammad Mansyur
AU  - Romi MM
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Saputri, Ramadhea Laila Afifa An-Nur Willya
AU  - Saputri RLAAW
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Rofiah, Edreana Khusnur
AU  - Rofiah EK
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Rahmanti, Trita
AU  - Rahmanti T
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Agustin, Maulidina
AU  - Agustin M
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
FAU - Sari, Dwi Cahyani Ratna
AU  - Sari DCR
AD  - Department of Anatomy, Faculty of Medicine, Public Health and Nursing Universitas 
      Gadjah Mada, Yogyakarta, Indonesia.
LA  - eng
PT  - Journal Article
DEP - 20200309
PL  - Japan
TA  - Kobe J Med Sci
JT  - The Kobe journal of medical sciences
JID - 0413531
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 1406-16-2 (Vitamin D)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/genetics
MH  - Inflammation/*prevention & control
MH  - Kidney/*blood supply/drug effects
MH  - Macrophages/drug effects
MH  - Male
MH  - Mice
MH  - Myofibroblasts/*drug effects
MH  - Reperfusion Injury/*prevention & control
MH  - Toll-Like Receptor 4/genetics
MH  - Vitamin D/pharmacology/*therapeutic use
PMC - PMC7447095
OTO - NOTNLM
OT  - Inflammation
OT  - Kidney IR injury
OT  - Myofibroblast
OT  - Tubular injury
OT  - Vitamin D
EDAT- 2020/03/24 06:00
MHDA- 2021/01/05 06:00
PMCR- 2020/03/09
CRDT- 2020/03/24 06:00
PHST- 2020/03/24 06:00 [entrez]
PHST- 2020/03/24 06:00 [pubmed]
PHST- 2021/01/05 06:00 [medline]
PHST- 2020/03/09 00:00 [pmc-release]
AID - kobej-65-e138 [pii]
PST - epublish
SO  - Kobe J Med Sci. 2020 Mar 9;65(4):E138-E143.