PMID- 32205215
OWN - NLM
STAT- MEDLINE
DCOM- 20210406
LR  - 20210406
IS  - 1873-6300 (Electronic)
IS  - 0891-0618 (Linking)
VI  - 106
DP  - 2020 Jul
TI  - Phenotypic characterization of MCP-1 expressing neurons in the rat cerebral 
      cortex.
PG  - 101785
LID - S0891-0618(20)30054-5 [pii]
LID - 10.1016/j.jchemneu.2020.101785 [doi]
AB  - Chemokines are small, secreted molecules that mediate inflammatory reactions. 
      Neurons and astrocytes constitutively express chemokines implicated in the 
      process of neuroinflammation associated with neurodegenerative diseases. The 
      monocyte chemoattractant protein-1 (MCP-1) has been widely related to this 
      process. However, the constitutive expression of this molecule by neurons has not 
      been elucidated so far. In this study, we set out to characterize the 
      neurochemical phenotype of MCP-1-expressing neurons in the rat neocortex to infer 
      its role in basal conditions. We observed the presence of two populations of 
      neurons expressing MCP-1: One population of cells with weak expression of MCP-1 
      corresponding to principal neurons (Tbr-1 positive) and a second population with 
      high expression of MCP-1 corresponding to inhibitory neurons (GAD-67 positive), 
      in particular to CCK/CBR1 interneurons. Moreover, high MCP-1-expressing neurons 
      were metabolically active (pCREB positive). The population of CCK interneurons 
      that co-localizes with MCP-1 corresponds to the regular-spiking basket cells and 
      is co-responsible for the perisomatic inhibition of principal pyramidal neurons. 
      Previous studies have demonstrated that MCP-1 can alter the electric properties 
      of neurons and a tonic function for this molecule has been postulated. As 
      CCK-inhibitory neurons are affected in mood disorders, whether the expression of 
      MCP-1 was maintained in humans could be part of the link between inflammatory 
      responses and observed changes in mood state.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Mulet, Maria
AU  - Mulet M
AD  - Cell Biology Department, Universitat de Valencia, Spain.
FAU - Blasco-Ibanez, Jose Miguel
AU  - Blasco-Ibanez JM
AD  - Cell Biology Department, Universitat de Valencia, Spain. Electronic address: 
      blascojm@uv.es.
FAU - Kirstein, Martina
AU  - Kirstein M
AD  - Cell Biology Department, Universitat de Valencia, Spain. Electronic address: 
      Martina.Kirstein@uv.es.
FAU - Crespo, Carlos
AU  - Crespo C
AD  - Cell Biology Department, Universitat de Valencia, Spain. Electronic address: 
      carlos.crespo@uv.es.
FAU - Nacher, Juan
AU  - Nacher J
AD  - Cell Biology Department, Universitat de Valencia, Spain; Fundacion Investigacion 
      Hospital Clinico de Valencia, INCLIVA, Spain; CIBERSAM: Spanish National Network 
      for Research in Mental Health, Spain. Electronic address: nacher@uv.es.
FAU - Varea, Emilio
AU  - Varea E
AD  - Cell Biology Department, Universitat de Valencia, Spain. Electronic address: 
      emilio.varea@uv.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200320
PL  - Netherlands
TA  - J Chem Neuroanat
JT  - Journal of chemical neuroanatomy
JID - 8902615
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Cerebral Cortex/*metabolism
MH  - Chemokine CCL2/*metabolism
MH  - Interneurons/metabolism
MH  - Neurons/*metabolism
MH  - Phenotype
MH  - Pyramidal Cells/metabolism
MH  - Rats
OTO - NOTNLM
OT  - CCL2
OT  - Interneuron
OT  - MCP-1
OT  - Neocortex
OT  - Phenotypic characterization
OT  - Rat
COIS- Declaration of Competing Interest The authors declare that they have no conflict 
      of interest.
EDAT- 2020/03/25 06:00
MHDA- 2021/04/07 06:00
CRDT- 2020/03/25 06:00
PHST- 2020/01/12 00:00 [received]
PHST- 2020/03/12 00:00 [revised]
PHST- 2020/03/14 00:00 [accepted]
PHST- 2020/03/25 06:00 [pubmed]
PHST- 2021/04/07 06:00 [medline]
PHST- 2020/03/25 06:00 [entrez]
AID - S0891-0618(20)30054-5 [pii]
AID - 10.1016/j.jchemneu.2020.101785 [doi]
PST - ppublish
SO  - J Chem Neuroanat. 2020 Jul;106:101785. doi: 10.1016/j.jchemneu.2020.101785. Epub 
      2020 Mar 20.