PMID- 32206066
OWN - NLM
STAT- MEDLINE
DCOM- 20201204
LR  - 20220413
IS  - 1689-1392 (Electronic)
IS  - 1425-8153 (Print)
IS  - 1425-8153 (Linking)
VI  - 25
DP  - 2020
TI  - MiR-206 may suppress non-small lung cancer metastasis by targeting CORO1C.
PG  - 22
LID - 10.1186/s11658-020-00216-x [doi]
LID - 22
AB  - OBJECT: Non-small lung cancer (NSCLC), with a poor 5-year survival rate (16%), is 
      the major type of lung cancer. Metastasis has been identified as the main factor 
      that leads to NSCLC therapy failure. MiR-206 is a metastasis suppressor in many 
      cancers, including colorectal cancer, renal cell carcinoma and breast cancer. 
      However, the role of miR-206 in NSCLC metastasis and the underlying mechanism are 
      still obscure. METHODS: Quantitative reverse-transcription PCR (q-RT-PCR) assay 
      was used to detect miR-206 mRNA of NSCLC tissues and lung cancer lines. The MTT 
      assay, scratch wound healing assay, transwell migration assay and transwell 
      invasion assay were conducted to illuminate the effect of miR-206 on A549 cells' 
      proliferation, migration and invasion. Gaussia luciferase reporter assay, 
      q-RT-PCR and western blotting assay were used to explore the underlying 
      mechanism. Also, the A549 xenograft model was conducted to evaluate the 
      anti-tumor effect of miR-206 in vivo. RESULTS: The results showed that miR-206 
      expression was decreased in NSCLC tissues and lung cancer cells. Further research 
      demonstrated that miR-206 inhibited the proliferation, migration and invasion of 
      A549 cells via negatively regulating Coronin-1C (CORO1C), and CORO1C deletion 
      significantly rescues the miR-206 mediated inhibitory effect on A549 cells. 
      Moreover, miR-206 exhibited a perfect anti-tumor effect in the A549 xenograft 
      model. CONCLUSION: Our study reveals that miR-206 functions as a tumor metastasis 
      suppressor and sheds new light on the clinical significance of miR-206 in NSCLC 
      therapy.
CI  - (c) The Author(s) 2020.
FAU - Liao, Ming
AU  - Liao M
AD  - Thoracic Surgery Department, General Hospital of Southern Theater Command, PLA, 
      No. 111, Liuhua Road, Yuexiu District, Guangzhou, 510010 China.
FAU - Peng, Lijun
AU  - Peng L
AD  - Thoracic Surgery Department, General Hospital of Southern Theater Command, PLA, 
      No. 111, Liuhua Road, Yuexiu District, Guangzhou, 510010 China.
LA  - eng
PT  - Journal Article
DEP - 20200317
PL  - England
TA  - Cell Mol Biol Lett
JT  - Cellular & molecular biology letters
JID - 9607427
RN  - 0 (MIRN206 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Microfilament Proteins)
RN  - 145420-64-0 (coronin proteins)
SB  - IM
MH  - A549 Cells
MH  - Animals
MH  - Carcinoma, Non-Small-Cell Lung/genetics/*metabolism/*secondary
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Down-Regulation
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Lung Neoplasms/genetics/*metabolism/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/genetics/*metabolism
MH  - Microfilament Proteins/genetics/*metabolism
MH  - Neoplasm Invasiveness/genetics
MH  - Up-Regulation
MH  - Xenograft Model Antitumor Assays
PMC - PMC7079403
OTO - NOTNLM
OT  - Coronin-1C (CORO1C)
OT  - Migration and invasion
OT  - Non-small-cell lung cancer (NSCLC)
OT  - Proliferation
OT  - microR-206 (miR-206)
COIS- Competing interestsThe authors declare that they have no competing interests.
EDAT- 2020/03/25 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/03/17
CRDT- 2020/03/25 06:00
PHST- 2019/09/15 00:00 [received]
PHST- 2020/03/06 00:00 [accepted]
PHST- 2020/03/25 06:00 [entrez]
PHST- 2020/03/25 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/03/17 00:00 [pmc-release]
AID - 216 [pii]
AID - 10.1186/s11658-020-00216-x [doi]
PST - epublish
SO  - Cell Mol Biol Lett. 2020 Mar 17;25:22. doi: 10.1186/s11658-020-00216-x. 
      eCollection 2020.