PMID- 32208000
OWN - NLM
STAT- MEDLINE
DCOM- 20200729
LR  - 20200729
IS  - 1522-1555 (Electronic)
IS  - 0193-1849 (Linking)
VI  - 318
IP  - 5
DP  - 2020 May 1
TI  - A translation repressor, 4E-BP1, regulates the triglyceride level in rat liver 
      during protein deprivation.
PG  - E636-E645
LID - 10.1152/ajpendo.00464.2019 [doi]
AB  - Protein deprivation has been shown to induce fatty liver in humans and animals, 
      but the molecular mechanisms underlying such induction are largely unknown. Our 
      previous studies have shown that a low-protein diet increases eukaryotic 
      translation initiation factor 4E-binding protein 1 (4E-BP1) protein and 
      triglyceride (TG) levels in rat liver. 4E-BP1 is known to repress translation by 
      binding to eIF4E. There is also evidence indicating that 4E-BP1 regulates lipid 
      metabolism. Here, we examined the role of 4E-BP1 on TG accumulation in the livers 
      of rats under protein deprivation. The low-protein diet rapidly increased the 
      hepatic 4E-BP1 mRNA level within 1 day, followed by the induction of hepatic TG 
      accumulation. The knockdown of hepatic 4E-BP1 attenuated the TG accumulation in 
      rat liver induced by the low-protein diet. 4E-BP1 knockdown also increased the 
      protein level of carnitine palmitoyltransferase 1A (CPT1A), a regulator of fatty 
      acid oxidation, in the liver of rats fed a low-protein diet. These results 
      indicate that a low-protein diet increases the amount of 4E-BP1, leading to TG 
      accumulation in rat liver. We thus conclude that 4E-BP1 plays an important role 
      in inducing hepatic steatosis under protein deprivation.
FAU - Toyoshima, Yuka
AU  - Toyoshima Y
AD  - Department of Bioregulation, Institute for Advanced Medical Sciences, Nippon 
      Medical School, Kawasaki, Kanagawa, Japan.
FAU - Yoshizawa, Fumiaki
AU  - Yoshizawa F
AD  - Department of Agrobiology and Bioresources, School of Agriculture, Utsunomiya 
      University, Utsunomiya, Tochigi, Japan.
FAU - Tokita, Reiko
AU  - Tokita R
AD  - Department of Bioregulation, Institute for Advanced Medical Sciences, Nippon 
      Medical School, Kawasaki, Kanagawa, Japan.
FAU - Taguchi, Yusuke
AU  - Taguchi Y
AD  - Department of Bioregulation, Institute for Advanced Medical Sciences, Nippon 
      Medical School, Kawasaki, Kanagawa, Japan.
FAU - Takahashi, Shin-Ichiro
AU  - Takahashi SI
AD  - Department of Animal Sciences, Graduate School of Agriculture and Life Sciences, 
      The University of Tokyo, Tokyo, Japan.
FAU - Kato, Hisanori
AU  - Kato H
AD  - Department of Applied Biological Chemistry, Graduate School of Agricultural and 
      Life Sciences, The University of Tokyo, Tokyo, Japan.
FAU - Minami, Shiro
AU  - Minami S
AD  - Department of Bioregulation, Institute for Advanced Medical Sciences, Nippon 
      Medical School, Kawasaki, Kanagawa, Japan.
LA  - eng
SI  - figshare/10.6084/m9.figshare.11925414.v1
SI  - figshare/10.6084/m9.figshare.11955315.v1
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200324
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Eif4ebp1 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Triglycerides)
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
RN  - EC 2.3.1.21 (carnitine palmitoyltransferase-1a, rat)
SB  - IM
MH  - Animals
MH  - Carnitine O-Palmitoyltransferase/metabolism
MH  - *Diet, Protein-Restricted
MH  - Gene Knockdown Techniques
MH  - Intracellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Liver/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Triglycerides/*metabolism
OTO - NOTNLM
OT  - 4E-BP1
OT  - liver
OT  - low-protein diet
OT  - rat
OT  - triglyceride
EDAT- 2020/03/26 06:00
MHDA- 2020/07/30 06:00
CRDT- 2020/03/26 06:00
PHST- 2020/03/26 06:00 [pubmed]
PHST- 2020/07/30 06:00 [medline]
PHST- 2020/03/26 06:00 [entrez]
AID - 10.1152/ajpendo.00464.2019 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2020 May 1;318(5):E636-E645. doi: 
      10.1152/ajpendo.00464.2019. Epub 2020 Mar 24.