PMID- 32208602 OWN - NLM STAT- MEDLINE DCOM- 20210211 LR - 20210211 IS - 1520-6882 (Electronic) IS - 0003-2700 (Linking) VI - 92 IP - 9 DP - 2020 May 5 TI - Metal-Labeled Aptamers as Novel Nanoprobes for Imaging Mass Cytometry Analysis. PG - 6312-6320 LID - 10.1021/acs.analchem.9b05159 [doi] AB - Imaging mass cytometry (IMC) is an emerging imaging technology that exploits the multiplexed analysis capabilities of the CyTOF mass cytometer to make spatially resolved measurements for tissue sections. In a comprehensive view of tissue composition and marker distribution, recent developments of IMC require highly sensitive, multiplexed assays. Approaching the sensitivity of the IMC technique, we designed a novel type of biocompatible metal-labeled aptamer nanoprobe (MAP), named (167)Er-A10-3.2. The small molecular probe was synthesized by conjugating (167)Er-polymeric pentetic acid ((167)Er-DTPA) with an RNA aptamer A10-3.2. For demonstration, (167)Er-A10-3.2 was applied for observing protein spatial distribution on prostatic epithelium cell of paraffin embedded Prostatic adenocarcinoma (PaC) tissue sections by IMC technology. The (167)Er-A10-3.2 capitalizes on the ability of the aptamer to specifically bind target cancer cells as well as the small size of (167)Er-A10-3.2 can accommodate multiple aptamer binding antigen labeled at high density. The detection signal of (167)Er-A10-3.2 probe was 3-fold higher than that of PSMA antibody probe for a targeted cell under lower temperature epitope retrieval (37 degrees C) of PaC tissue. Furthermore, we successfully demonstrated the simultaneously staining ability of aptamer probes in IMC analysis. The successful imaging acquisition using aptamers probes in IMC technology may offer opportunity for the diagnosis of malignancies in the future. FAU - Yu, Youyi AU - Yu Y AD - Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. FAU - Dang, Jingqi AU - Dang J AD - Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. FAU - Liu, Xiao AU - Liu X AD - Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. FAU - Wang, Liping AU - Wang L AD - Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. FAU - Li, Shanhe AU - Li S AD - Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. FAU - Zhang, Ting AU - Zhang T AD - Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. FAU - Ding, Xianting AU - Ding X AUID- ORCID: 0000-0002-1549-3499 AD - Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200401 PL - United States TA - Anal Chem JT - Analytical chemistry JID - 0370536 RN - 0 (Antibodies) RN - 0 (Antigens, Surface) RN - 0 (Aptamers, Nucleotide) RN - 77B218D3YE (Erbium) RN - EC 3.4.17.21 (FOLH1 protein, human) RN - EC 3.4.17.21 (Glutamate Carboxypeptidase II) RN - EC 3.4.21.77 (Prostate-Specific Antigen) SB - IM MH - Antibodies/chemistry/immunology MH - Antigens, Surface/immunology/metabolism MH - Aptamers, Nucleotide/*chemistry/metabolism MH - Erbium/*chemistry MH - Glutamate Carboxypeptidase II/immunology/metabolism MH - Humans MH - Image Cytometry/*methods MH - Male MH - Prostate-Specific Antigen/immunology/metabolism MH - Prostatic Neoplasms/pathology EDAT- 2020/03/27 06:00 MHDA- 2021/02/12 06:00 CRDT- 2020/03/27 06:00 PHST- 2020/03/27 06:00 [pubmed] PHST- 2021/02/12 06:00 [medline] PHST- 2020/03/27 06:00 [entrez] AID - 10.1021/acs.analchem.9b05159 [doi] PST - ppublish SO - Anal Chem. 2020 May 5;92(9):6312-6320. doi: 10.1021/acs.analchem.9b05159. Epub 2020 Apr 1.