PMID- 32208888
OWN - NLM
STAT- MEDLINE
DCOM- 20210730
LR  - 20230317
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 19
IP  - 9
DP  - 2020 May
TI  - Exosomes-carried microRNA-26b-5p regulates microglia M1 polarization after 
      cerebral ischemia/reperfusion.
PG  - 1022-1035
LID - 10.1080/15384101.2020.1743912 [doi]
AB  - Exosome and microRNAs (miRs) are implicated in ischemia/reperfusion (I/R) 
      process. In this study, I/R mouse model was established, and exosomes derived 
      from human umbilical cord mesenchymal stem cells (hUCMSCs) were isolated, 
      identified, and injected to I/R mice to observe nerve injury and microglia M1 
      polarization. The differentially expressed genes in I/R microglia from databases 
      were analyzed, and miRs differentially expressed in exosomes-treated microglia 
      were analyzed by microarray. miR-26b-5p expression in hUCMSCs was intervened. 
      Besides, microglia was extracted and co-cultured with SH-SY5Y or PC12 cells in 
      oxygen-glucose deprivation/reperfusion (OGD/R) models to simulate I/R in vivo. 
      Additionally, Toll-like receptor (TLR) activator GS-9620 was added to microglia. 
      Exosomes alleviated nerve injury and inhibited M1 polarization in microglia. 
      After I/R modeling, CH25H expression in microglia was upregulated but decreased 
      after exosome treatment. miR-26b-5p was upregulated in microglia after exosome 
      treatment and could target CH25H. Reduction in exosomal miR-26b-5p reversed the 
      effects of hUCMSCs-exos on microglia. TLR pathway was activated in microglia 
      after I/R but exosomes prevented its activation. Exosomal miR-26b-5p could 
      repress M1 polarization of microglia by targeting CH25H to inactivate the TLR 
      pathway, so as to relieve nerve injury after cerebral I/R. This investigation may 
      offer new approaches for I/R treatment.
FAU - Li, Guangying
AU  - Li G
AD  - Department of Neurosurgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      P.R. China.
FAU - Xiao, Longhai
AU  - Xiao L
AD  - Department of Neurosurgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      P.R. China.
FAU - Qin, Hao
AU  - Qin H
AD  - Department of Neurosurgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      P.R. China.
FAU - Zhuang, Qiang
AU  - Zhuang Q
AD  - Department of Neurosurgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      P.R. China.
FAU - Zhang, Weiwei
AU  - Zhang W
AD  - Department of Neurosurgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      P.R. China.
FAU - Liu, Long
AU  - Liu L
AD  - Department of Neurosurgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      P.R. China.
FAU - Di, Chao
AU  - Di C
AD  - Department of Neurosurgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      P.R. China.
FAU - Zhang, Yabo
AU  - Zhang Y
AD  - Department of Neurosurgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      P.R. China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20200325
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (MIRN26A microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 1.14.- (Steroid Hydroxylases)
RN  - EC 1.14.99.38 (cholesterol 25-hydroxylase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
RIN - Cell Cycle. 2023 May;22(9):1156. PMID: 36927286
MH  - Animals
MH  - Brain Ischemia/*metabolism
MH  - Cell Hypoxia
MH  - Cell Polarity/*genetics
MH  - Coculture Techniques
MH  - Disease Models, Animal
MH  - Exosomes/*metabolism
MH  - Glucose/deficiency
MH  - Humans
MH  - Mesenchymal Stem Cells/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/*metabolism
MH  - Microglia/*metabolism
MH  - PC12 Cells
MH  - Rats
MH  - Reperfusion Injury/*metabolism
MH  - Steroid Hydroxylases/metabolism
MH  - Transfection
PMC - PMC7217376
OTO - NOTNLM
OT  - CH25H
OT  - Ischemia/reperfusion
OT  - M1 microglia
OT  - exosome
OT  - human umbilical cord mesenchymal stem cell
OT  - microRNA-26b-5p
EDAT- 2020/03/27 06:00
MHDA- 2021/07/31 06:00
PMCR- 2021/03/25
CRDT- 2020/03/27 06:00
PHST- 2020/03/27 06:00 [pubmed]
PHST- 2021/07/31 06:00 [medline]
PHST- 2020/03/27 06:00 [entrez]
PHST- 2021/03/25 00:00 [pmc-release]
AID - 1743912 [pii]
AID - 10.1080/15384101.2020.1743912 [doi]
PST - ppublish
SO  - Cell Cycle. 2020 May;19(9):1022-1035. doi: 10.1080/15384101.2020.1743912. Epub 
      2020 Mar 25.