PMID- 32209332 OWN - NLM STAT- MEDLINE DCOM- 20201222 LR - 20201222 IS - 1873-2933 (Electronic) IS - 0009-9120 (Linking) VI - 80 DP - 2020 Jun TI - Interleukin-18 promoter -137 G/C polymorphism (rs187238) is associated with biochemical markers of renal function and cardiovascular disease in type 2 diabetes patients. PG - 1-7 LID - S0009-9120(19)31135-X [pii] LID - 10.1016/j.clinbiochem.2020.03.011 [doi] AB - BACKGROUND: Interleukin-18 (IL-18), a proinflammatory and proatherogenic cytokine, has been associated with type 2 diabetes, metabolic syndrome, stroke and coronary artery disease. Some studies have indicated that the IL-18 promoter -137 G/C polymorphism seems to be associated with changes in the IL-18 expression and may contribute to the development of cardiovascular disease (CVD). The aim of this study was to evaluate the association between -137 G/C polymorphism and the levels of IL-18, biochemical markers for cardiovascular disorders, anthropometric profile and cardiovascular disease in Brazilian patients with type 2 diabetes (T2DM). DESIGN & METHODS: Study subjects comprised 125 T2DM patients undergoing follow-up at a reference endocrinology service in northeastern Brazil. The -137G/C polymorphism in the IL-18 gene and serum IL-18 levels were determined by using allele-specific polymerase chain reaction (PCR) and enzyme-linked immune assay (ELISA), respectively. The anthropometric parameters were assessed using a Body Composition Monitor with Scale, and the laboratory data were measured using an automatic analyzer as well as spectrophotometric analysis. RESULTS: The genotype distribution of IL-18 -137 G/C genetic polymorphism was significantly different among T2DM patients with and without CVD. The results show an association between the CC genotype of -137G/C polymorphism and CVD in T2DM patients (p < 0.001). Serum levels of IL-18 were significantly higher in CC carriers (843.1 pg/mL) compared with GG or GC carriers (303.6 pg/mL and 292.0 pg/mL, respectively). In addition, the present study showed that carriers of the CC genotype also had significantly higher concentrations of creatinine and albuminuria than carriers of the GG or GC genotypes (p < 0.05 in both). CONCLUSION: These results suggest that Brazilian T2DM patients with the CC genotype seem to show a predisposition to CVD, as well as an elevation in markers of renal function. CI - Published by Elsevier Inc. FAU - Cavalcante, Janio Emanuel Andrade AU - Cavalcante JEA AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. Electronic address: janiofarma@yahoo.com.br. FAU - de Sousa, Ederson Laurindo Holanda AU - de Sousa ELH AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. Electronic address: ederson.laurindo@hotmail.com. FAU - de Oliveira Rodrigues, Raphael AU - de Oliveira Rodrigues R AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - de Almeida Viana, Glautemberg AU - de Almeida Viana G AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Duarte Gadelha, Daniel AU - Duarte Gadelha D AD - Endocrinology and Diabetes Service at Walter Cantidio University Hospital, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - de Carvalho, Manoela Montenegro Dias AU - de Carvalho MMD AD - Endocrinology and Diabetes Service at Walter Cantidio University Hospital, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Sousa, Duaran Lopes AU - Sousa DL AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Silva, Allysson Jordan Xavier AU - Silva AJX AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Filho, Raimundo Rigoberto Barbosa Xavier AU - Filho RRBX AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Fernandes, Virginia Oliveira AU - Fernandes VO AD - Endocrinology and Diabetes Service at Walter Cantidio University Hospital, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Montenegro Junior, Renan Magalhaes AU - Montenegro Junior RM AD - Endocrinology and Diabetes Service at Walter Cantidio University Hospital, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - de Sousa Alves, Renata AU - de Sousa Alves R AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Meneses, Gdayllon Cavalcante AU - Meneses GC AD - Medical Sciences Post-Graduate Program, Department of Internal Medicine, School of Medicine, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Sampaio, Tiago Lima AU - Sampaio TL AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. FAU - Queiroz, Maria Goretti Rodrigues AU - Queiroz MGR AD - Department of Clinical and Toxicological Analysis, Federal University of Ceara, Fortaleza, Ceara, Brazil. LA - eng PT - Journal Article DEP - 20200321 PL - United States TA - Clin Biochem JT - Clinical biochemistry JID - 0133660 RN - 0 (Biomarkers) RN - 0 (Interleukin-18) SB - IM MH - Adult MH - Aged MH - Biomarkers/blood MH - Brazil/epidemiology MH - Cardiovascular Diseases/epidemiology/*genetics MH - Diabetes Mellitus, Type 2/epidemiology/*genetics MH - Female MH - Genetic Predisposition to Disease MH - Humans MH - Interleukin-18/*genetics MH - Male MH - Middle Aged MH - Polymorphism, Single Nucleotide MH - *Promoter Regions, Genetic MH - Renal Insufficiency/epidemiology/*genetics OTO - NOTNLM OT - Cardiovascular disease OT - Diabetes mellitus OT - Interleukin-18 OT - Single nucleotide polymorphism COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2020/03/27 06:00 MHDA- 2020/12/23 06:00 CRDT- 2020/03/27 06:00 PHST- 2019/10/17 00:00 [received] PHST- 2020/02/17 00:00 [revised] PHST- 2020/03/17 00:00 [accepted] PHST- 2020/03/27 06:00 [pubmed] PHST- 2020/12/23 06:00 [medline] PHST- 2020/03/27 06:00 [entrez] AID - S0009-9120(19)31135-X [pii] AID - 10.1016/j.clinbiochem.2020.03.011 [doi] PST - ppublish SO - Clin Biochem. 2020 Jun;80:1-7. doi: 10.1016/j.clinbiochem.2020.03.011. Epub 2020 Mar 21.