PMID- 32210595
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220413
IS  - 1178-6973 (Print)
IS  - 1178-6973 (Electronic)
IS  - 1178-6973 (Linking)
VI  - 13
DP  - 2020
TI  - Dendritic Cells Promote Treg Expansion but Not Th17 Generation in Response to 
      Talaromyces marneffei Yeast Cells.
PG  - 805-813
LID - 10.2147/IDR.S239906 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) with both proinflammatory and tolerogenic 
      properties have been implicated in modulation of CD4(+) T cell responses in many 
      fungal diseases. However, the role of DC in the context of Talaromyces marneffei 
      (T. marneffei) infection has not been determined. In this study, we aimed to 
      study the effect of the yeast form of T. marneffei yeasts on DCs, as well as the 
      role of DCs in modulating T helper 17 (Th17) and regulatory T (Treg) cell 
      responses to the pathogen. METHODS: Mouse bone marrow-derived DCs were stimulated 
      with T. marneffei yeasts for 24 h. Frequencies of CD80 and CD86 expression on DCs 
      and the levels of IL-6, IL-10 and TGF-beta in the culture supernatant of 
      yeast-stimulated DCs were detected by flow cytometry and ELISA, respectively. In 
      co-culture experiments, CD4(+) T lymphocytes of mice were isolated from the 
      spleen using magnetic beads and co-cultured with T. marneffei yeasts, with or 
      without DCs for 24 h. The proportions of Th17 and Treg cells in co-culture were 
      detected by flow cytometry. The mRNA levels of RORgammat and Foxp3 were detected by 
      RT-PCR. Levels of IL-10 and TGF-beta in the co-culture supernatant were detected by 
      ELISA. RESULTS: The expressions of CD80 and CD86 on DCs were increased, as well 
      as IL-6, IL-10 and TGF-beta levels in the culture supernatant of T. 
      marneffei-stimulated DCs were higher than those in DCs cultured without T. 
      marneffei. In co-culture experiments, in the presence of DCs, T. marneffei 
      promoted Treg expansion and Foxp3 up-regulation but limited Th17 and 
      downregulated RORgammat. Levels of IL-10 and TGF-beta were higher in the co-culture 
      containing DCs than without DCs. CONCLUSION: Our findings demonstrated that the 
      interaction between DCs and T. marneffei could promote Treg expansion but not 
      Th17 generation. These findings provide a mechanism by which DCs may promote 
      immune tolerance in T. marneffei infection.
CI  - (c) 2020 Tang et al.
FAU - Tang, Yanping
AU  - Tang Y
AD  - Department of Respiratory and Critical Care Medicine, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of 
      China.
FAU - Zhang, Hui
AU  - Zhang H
AD  - Department of Respiratory and Critical Care Medicine, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of 
      China.
FAU - Xu, Haiguang
AU  - Xu H
AD  - Department of Respiratory and Critical Care Medicine, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of 
      China.
FAU - Zeng, Wen
AU  - Zeng W
AD  - Department of Respiratory and Critical Care Medicine, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of 
      China.
FAU - Qiu, Ye
AU  - Qiu Y
AD  - Department of Respiratory and Critical Care Medicine, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of 
      China.
FAU - Tan, Caimei
AU  - Tan C
AD  - Department of Respiratory and Critical Care Medicine, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of 
      China.
FAU - Tang, Shudan
AU  - Tang S
AUID- ORCID: 0000-0003-3656-3895
AD  - Department of Respiratory and Critical Care Medicine, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of 
      China.
FAU - Zhang, Jianquan
AU  - Zhang J
AD  - Department of Respiratory and Critical Care Medicine, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200311
PL  - New Zealand
TA  - Infect Drug Resist
JT  - Infection and drug resistance
JID - 101550216
PMC - PMC7075240
OTO - NOTNLM
OT  - Talaromyces marneffei
OT  - Th17 cells
OT  - dendritic cells
OT  -  Treg cells
COIS- No conflicts of interest, financial or otherwise, are declared by the authors.
EDAT- 2020/03/27 06:00
MHDA- 2020/03/27 06:01
PMCR- 2020/03/11
CRDT- 2020/03/27 06:00
PHST- 2019/11/25 00:00 [received]
PHST- 2020/02/25 00:00 [accepted]
PHST- 2020/03/27 06:00 [entrez]
PHST- 2020/03/27 06:00 [pubmed]
PHST- 2020/03/27 06:01 [medline]
PHST- 2020/03/11 00:00 [pmc-release]
AID - 239906 [pii]
AID - 10.2147/IDR.S239906 [doi]
PST - epublish
SO  - Infect Drug Resist. 2020 Mar 11;13:805-813. doi: 10.2147/IDR.S239906. eCollection 
      2020.