PMID- 32210713
OWN - NLM
STAT- MEDLINE
DCOM- 20201217
LR  - 20201217
IS  - 1449-1907 (Electronic)
IS  - 1449-1907 (Linking)
VI  - 17
IP  - 5
DP  - 2020
TI  - Water extract of tendril of Cucurbita Moschata Duch. suppresses RANKL-induced 
      osteoclastogenesis by down-regulating p38 and ERK signaling.
PG  - 632-639
LID - 10.7150/ijms.39622 [doi]
AB  - Background: Pumpkin (Curcubita sp.) is a natural product that is commonly used in 
      folk medicine. However, the inhibitory effect and molecular mechanisms of tendril 
      of Cucurbita Moschata Duch. (TCMD) on osteoclast differentiation have yet to be 
      clearly elucidated. Thus, the present study aimed to investigate the effect and 
      underlying mechanism of water extract of TCMD on osteoclast differentiation. 
      Methods: Bone marrow-derived macrophages (BMDMs), osteoclast precursors, were 
      cultured with macrophage colony stimulating factor (M-CSF) 30 ng/ml and receptor 
      activator of nuclear factor-kappa B ligand (RANKL) 100 ng/ml for four days. We 
      investigated the effect of TCMD on RANKL-induced osteoclast differentiation, 
      tartrate-resistant acid phosphatase (TRAP) staining, F-actin ring formation, and 
      bone resorption assay. RANKL signaling pathways were determined through Western 
      blotting, and osteoclast differentiation marker genes were confirmed by Real-time 
      PCR. Results: TCMD inhibited the RANKL-induced osteoclast differentiation in a 
      dose-dependent manner without cytotoxicity. Further, F-actin ring formation and 
      bone resorption were reduced by TCMD in RANKL-treated BMDMs. In addition, TCMD 
      decreased the phosphorylation of p38 and ERK as well as the expression of 
      osteoclast-related genes in BMDMs treated with RANKL. Conclusion: These findings 
      suggest that TCMD may have preventive and therapeutic effects for destructive 
      bone diseases.
CI  - (c) The author(s).
FAU - Choi, Joo-Hee
AU  - Choi JH
AD  - Laboratory Animal Medicine, College of Veterinary Medicine and BK 21 PLUS Project 
      Team, Chonnam National University, Gwangju, Republic of Korea.
AD  - Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, 
      Republic of Korea.
FAU - Jang, Ah-Ra
AU  - Jang AR
AD  - Laboratory Animal Medicine, College of Veterinary Medicine and BK 21 PLUS Project 
      Team, Chonnam National University, Gwangju, Republic of Korea.
FAU - Jeong, Ha-Na
AU  - Jeong HN
AD  - Department of Food Science & Technology, Chonnam National University, Gwangju, 
      Republic of Korea.
FAU - Kim, Kiok
AU  - Kim K
AD  - Department of Food Science & Technology, Chonnam National University, Gwangju, 
      Republic of Korea.
FAU - Kim, Young-Min
AU  - Kim YM
AD  - Department of Food Science & Technology, Chonnam National University, Gwangju, 
      Republic of Korea.
FAU - Cho, Jeong-Yong
AU  - Cho JY
AD  - Department of Food Science & Technology, Chonnam National University, Gwangju, 
      Republic of Korea.
FAU - Park, Jong-Hwan
AU  - Park JH
AD  - Laboratory Animal Medicine, College of Veterinary Medicine and BK 21 PLUS Project 
      Team, Chonnam National University, Gwangju, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20200224
PL  - Australia
TA  - Int J Med Sci
JT  - International journal of medical sciences
JID - 101213954
RN  - 0 (Plant Extracts)
RN  - 0 (RANK Ligand)
SB  - IM
MH  - Animals
MH  - Bone Resorption/*drug therapy
MH  - Cell Differentiation/drug effects
MH  - *Cucurbita
MH  - Drug Evaluation, Preclinical
MH  - MAP Kinase Signaling System/drug effects
MH  - Male
MH  - Mice
MH  - Osteoclasts/*drug effects
MH  - Osteogenesis/*drug effects
MH  - Phytotherapy
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - Primary Cell Culture
MH  - RANK Ligand
PMC - PMC7085206
OTO - NOTNLM
OT  - Osteoclast differentiation
OT  - RANKL
OT  - Tendril of Cucurbita Moschata Duch. (TCMD)
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2020/03/27 06:00
MHDA- 2020/12/18 06:00
PMCR- 2020/01/01
CRDT- 2020/03/27 06:00
PHST- 2019/08/25 00:00 [received]
PHST- 2020/02/12 00:00 [accepted]
PHST- 2020/03/27 06:00 [entrez]
PHST- 2020/03/27 06:00 [pubmed]
PHST- 2020/12/18 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - ijmsv17p0632 [pii]
AID - 10.7150/ijms.39622 [doi]
PST - epublish
SO  - Int J Med Sci. 2020 Feb 24;17(5):632-639. doi: 10.7150/ijms.39622. eCollection 
      2020.