PMID- 32210829
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 11
DP  - 2020
TI  - Comparative Secretome Analysis of Mesenchymal Stem Cells From Dental Apical 
      Papilla and Bone Marrow During Early Odonto/Osteogenic Differentiation: Potential 
      Role of Transforming Growth Factor-beta2.
PG  - 41
LID - 10.3389/fphys.2020.00041 [doi]
LID - 41
AB  - To understand the functions of secretory proteins in odontogenesis and to further 
      the understanding of the different molecular events during odontogenesis and 
      osteogenesis, we induced the odonto/osteogenic differentiation of stem cells from 
      dental apical papilla (SCAPs) and bone marrow-derived stem cells (BMSCs) in vitro 
      and compared the expression of secretory proteins during early odonto/osteogenic 
      differentiation using high-performance liquid chromatography with tandem mass 
      spectrometry. The results revealed significant changes by at least 50% in 139 
      SCAP proteins and 203 BMSC proteins during differentiation. Of these, 92 were 
      significantly upregulated and 47 were significantly downregulated during the 
      differentiation of SCAPs. Most of these proteins showed the same trend during the 
      differentiation of BMSCs. Among the proteins that showed significantly changes 
      during the differentiation of SCAPs and BMSCs, we found that transforming growth 
      factor-beta2 (TGFbeta2) is a key protein in the network with powerful mediation 
      ability. TGFbeta2 was secreted more by SCAPs than BMSCs, was significantly 
      upregulated during the differentiation of SCAPs and was significantly 
      downregulated during the differentiation of BMSCs. Furthermore, the effects of 
      recombinant human TGFbeta2 and TGFbeta1 on the odonto/osteogenic differentiation of 
      SCAPs and BMSCs were investigated. Real-time reverse transcription polymerase 
      chain reaction (RT-PCR) and western blotting data revealed that TGFbeta2 enhanced 
      the odontogenic-related markers [dentin sialophosphoprotein (DSPP), dentin matrix 
      protein 1 (DMP1)] and inhibited the osteogenic-related marker bone sialoprotein 
      (BSP) in SCAPs, whereas TGFbeta1 enhanced the BSP expression and inhibited the DSPP 
      and DMP1 expression at early odonto/osteogenic differentiation of SCAPs. However, 
      in BMSCs, TGFbeta2 enhanced the expression of alkaline phosphatase (ALP), 
      runt-related transcription factor 2 (RUNX2), DSPP, and DMP1, whereas TGFbeta1 
      enhanced the expression of ALP and RUNX2, with no significant intergroup 
      difference of DSPP at the early odonto/osteogenic differentiation of BMSCs. TGFbeta2 
      is a potentially important molecule with a distinct function in the regulation of 
      odontogenesis and osteogenesis.
CI  - Copyright (c) 2020 Yu, Li, Zhao, Li and Ge.
FAU - Yu, Shi
AU  - Yu S
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
FAU - Li, Jingzhi
AU  - Li J
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
FAU - Zhao, Yuming
AU  - Zhao Y
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
FAU - Li, Xiaoxia
AU  - Li X
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
FAU - Ge, Lihong
AU  - Ge L
AD  - Department of Pediatric Dentistry, Peking University School and Hospital of 
      Stomatology, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20200306
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC7073820
OTO - NOTNLM
OT  - bone marrow-derived stem cells (BMSCs)
OT  - odonto/osteogenic differentiation
OT  - secretory proteins
OT  - stem cells from dental apical papilla (SCAPs)
OT  - transforming growth factor-beta2 (TGFbeta2)
EDAT- 2020/03/27 06:00
MHDA- 2020/03/27 06:01
PMCR- 2020/03/06
CRDT- 2020/03/27 06:00
PHST- 2019/11/21 00:00 [received]
PHST- 2020/01/20 00:00 [accepted]
PHST- 2020/03/27 06:00 [entrez]
PHST- 2020/03/27 06:00 [pubmed]
PHST- 2020/03/27 06:01 [medline]
PHST- 2020/03/06 00:00 [pmc-release]
AID - 10.3389/fphys.2020.00041 [doi]
PST - epublish
SO  - Front Physiol. 2020 Mar 6;11:41. doi: 10.3389/fphys.2020.00041. eCollection 2020.