PMID- 32212454
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220413
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 24
IP  - 9
DP  - 2018 Sep
TI  - [Expressions of pannexin proteins in I-10 Leydig tumor cells and TM3 Leydig cells 
      and their regulatory effect on the channel function in mice].
PG  - 776-781
AB  - OBJECTIVE: To investigate the expressions of the pannexin (Panx) proteins in I-10 
      Leydig tumor cells and TM3 Leydig cells and their regulatory effect on the Panx 
      channel function in mice. METHODS: The expressions of the Panx-1 and Panx-2 
      proteins in the mouse Leydig tumor cells were determined by Western blot. The 
      I-10 Leydig tumor cells were treated with carbenoxolone (CBX) at 100 mumol/L or 
      probenecid (PBN) at 200 mumol/L, the fluorescence resonance energy transfer (FRET) 
      detected by time-lapse fluorescence imaging, and the extracellular adenosine 
      5'-triphosphate (eATP) level measured with the commercial detection kit. 
      Molecular biological methods were used to interfere with shRNA and overexpress 
      mPanx-1 the Panx-1 gene and regulate the expression and function of the Panx-1 
      protein. RESULTS: The expressions of Panx-1 (［289.5 +/- 55.8］%) and Panx-2 (［264.5 
      +/- 24.6］%) were significantly increased in the I-10 Leydig tumor cells as compared 
      with those in the normal TM3 Leydig cells (both P < 0.05). FRET was remarkably 
      reduced after treated with CBX (［87.5 +/- 17.7］%) and PBN (［89.3 +/- 14.3］%) in 
      comparison with that in the control group (both P < 0.01). At 8, 16 and 24 hours, 
      the eATP level was decreased by (57.3 +/- 7.2)%, (56.4 +/- 9.6)% and (63.4 +/- 6.4)% in 
      the CBX group (P < 0.01) and (61.7 +/- 2.5)%, (35.8 +/- 1.6)% and (13.5 +/- 8.3)% in 
      the PBN group (P < 0.01). Molecular biological treatment down-regulated the 
      expression of Panx-1 by (38.3 +/- 5.2)% and (31.8 +/- 5.1)% in the shRNA1 and shRNA2 
      groups, respectively (both P < 0.01), but up-regulated that of Panx-1 by (128.4 +/- 
      7.5)% in the mPanx-1 group (P < 0.01) as compared with the negative control. FRET 
      was reduced by (72.4 +/- 39.4)% in the shRNA group (P < 0.01) and the eATP level by 
      (14.7 +/- 0.1)%, (13.7 +/- 0.3)% and (13.1 +/- 0.3)% at 8, 16 and 24 hours, 
      respectively (P < 0.01) while FRET elevated by (122.5 +/- 17.1)% in the mPanx-1 
      group (P < 0.01) and the eATP level by (886.1 +/- 82.1)%, (885.8 +/- 83.3)% and 
      (841.5 +/- 21.8)% at 8, 16 and 24 hours, respectively (P < 0.01). CONCLUSIONS: The 
      expressions of Panx-1 and Panx-2 are increased in I-10 mouse Leydig tumor cells, 
      and inhibiting the Panx channel with CBX, PBN and shRNA reduces FRET and the eATP 
      level in the I-10 cells.
FAU - Liu, Hao-Feng
AU  - Liu HF
AD  - Faculty of Pharmacy, School of Pharmacology, Bengbu Medical College, Bengbu, 
      Anhui 233030, China.
FAU - Dong, Shu-Ying
AU  - Dong SY
AD  - Faculty of Pharmacy, School of Pharmacology, Bengbu Medical College, Bengbu, 
      Anhui 233030, China.
FAU - Yuan, Min
AU  - Yuan M
AD  - Faculty of Pharmacy, School of Pharmacology, Bengbu Medical College, Bengbu, 
      Anhui 233030, China.
FAU - Wang, Xue-Ru
AU  - Wang XR
AD  - Faculty of Pharmacy, School of Pharmacology, Bengbu Medical College, Bengbu, 
      Anhui 233030, China.
FAU - Wu, Dan-Dan
AU  - Wu DD
AD  - Faculty of Pharmacy, School of Pharmacology, Bengbu Medical College, Bengbu, 
      Anhui 233030, China.
FAU - Fan, Wei-Zhen
AU  - Fan WZ
AD  - Faculty of Pharmacy, School of Pharmacology, Bengbu Medical College, Bengbu, 
      Anhui 233030, China.
FAU - Tong, Xu-Hui
AU  - Tong XH
AD  - Faculty of Pharmacy, School of Pharmacology, Bengbu Medical College, Bengbu, 
      Anhui 233030, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
SB  - IM
OTO - NOTNLM
OT  - adenosine 5'-triphosphate
OT  - carbenoxolone
OT  - mouse
OT  - probenecid
OT  - pannexin channel
EDAT- 2018/09/01 00:00
MHDA- 2018/09/01 00:01
CRDT- 2020/03/27 06:00
PHST- 2020/03/27 06:00 [entrez]
PHST- 2018/09/01 00:00 [pubmed]
PHST- 2018/09/01 00:01 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2018 Sep;24(9):776-781.