PMID- 32216615
OWN - NLM
STAT- MEDLINE
DCOM- 20200713
LR  - 20200930
IS  - 1522-1539 (Electronic)
IS  - 0363-6135 (Linking)
VI  - 318
IP  - 5
DP  - 2020 May 1
TI  - Macrophage galectin-3 enhances intimal translocation of vascular calcification in 
      diabetes mellitus.
PG  - H1068-H1079
LID - 10.1152/ajpheart.00690.2019 [doi]
AB  - The clinical risks and prognosis of diabetic vascular intimal calcification (VIC) 
      and medial calcification (VMC) are different. This study aims to investigate the 
      mechanism of VIC/VMC translocation. Anterior tibial arteries were collected from 
      patients with diabetic foot amputation. The patients were then divided into VIC 
      and VMC groups. There were plaques in all anterior tibial arteries, while the 
      enrichment of galectin-3 in arterial plaques in the VIC group was significantly 
      higher than that in the VMC group. Furthermore, a macrophage/vascular smooth 
      muscle cell (VSMC) coculture system was constructed. VSMC-derived extracellular 
      vesicles (EVs) was labeled with fluorescent probe. After macrophages were 
      pretreated with recombinant galectin-3 protein, the migration of VSMC-derived EVs 
      and VSMC-derived calcification was more pronounced. And anti-galectin-3 antibody 
      can inhibit this process of EVs and calcification translocation. Then, lentivirus 
      (LV)-treated bone marrow cells (BMCs) were transplanted into apolipoprotein 
      E-deficient (ApoE(-/-)) mice, and a diabetic atherosclerosis mouse model was 
      constructed. After 15 wk of high-fat diet, ApoE(-/-) mice transplanted with 
      LV-shgalectin-3 BMCs exhibited medial calcification and a concentrated 
      distribution of EVs in the media. In conclusion, upregulation of galectin-3 in 
      macrophages promotes the migration of VSMC-derived EVs to the intima and induces 
      diabetic vascular intimal calcification.NEW & NOTEWORTHY The clinical risk and 
      prognosis of vascular intimal and medial calcification are different. Macrophage 
      galectin-3 regulates the migration of vascular smooth muscle cell-derived 
      extracellular vesicles and mediates diabetic vascular intimal/medial 
      calcification translocation. This study may provide insights into the early 
      intervention in diabetic vascular calcification.
FAU - Sun, Zhen
AU  - Sun Z
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Li, Lihua
AU  - Li L
AD  - Department of Pathology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Zhang, Lili
AU  - Zhang L
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Yan, Jinchuan
AU  - Yan J
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Shao, Chen
AU  - Shao C
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Bao, Zhengyang
AU  - Bao Z
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Liu, Jia
AU  - Liu J
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Li, Yalan
AU  - Li Y
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Zhou, Mengxue
AU  - Zhou M
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Hou, Lina
AU  - Hou L
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Jing, Lele
AU  - Jing L
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Pang, Qiwen
AU  - Pang Q
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Geng, Yue
AU  - Geng Y
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Mao, Xiang
AU  - Mao X
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Gu, Wen
AU  - Gu W
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
FAU - Wang, Zhongqun
AU  - Wang Z
AD  - Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200327
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
RN  - 0 (Apolipoproteins E)
RN  - 0 (Galectin 3)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Apolipoproteins E/genetics
MH  - Cells, Cultured
MH  - Diabetic Angiopathies/*metabolism/pathology
MH  - Diabetic Cardiomyopathies/genetics/*metabolism/pathology
MH  - Extracellular Vesicles/metabolism
MH  - Female
MH  - Galectin 3/*metabolism
MH  - Humans
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Tibial Arteries/metabolism/pathology
MH  - Tunica Intima/*metabolism/pathology
MH  - Vascular Calcification/*metabolism/pathology
OTO - NOTNLM
OT  - extracellular vesicle
OT  - galectin-3
OT  - macrophage
OT  - vascular calcification
OT  - vascular smooth muscle cell
EDAT- 2020/03/29 06:00
MHDA- 2020/07/14 06:00
CRDT- 2020/03/29 06:00
PHST- 2020/03/29 06:00 [pubmed]
PHST- 2020/07/14 06:00 [medline]
PHST- 2020/03/29 06:00 [entrez]
AID - 10.1152/ajpheart.00690.2019 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2020 May 1;318(5):H1068-H1079. doi: 
      10.1152/ajpheart.00690.2019. Epub 2020 Mar 27.