PMID- 32218180
OWN - NLM
STAT- MEDLINE
DCOM- 20210415
LR  - 20210415
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 10
IP  - 4
DP  - 2020 Mar 25
TI  - Proteomic Analysis of Blood Exosomes from Healthy Females and Breast Cancer 
      Patients Reveals an Association between Different Exosomal Bioactivity on 
      Non-tumorigenic Epithelial Cell and Breast Cancer Cell Migration in Vitro.
LID - 10.3390/biom10040495 [doi]
LID - 495
AB  - Exosomes are important intercellular communication vehicles, secreted into body 
      fluids by multiple cell types, including tumor cells. They contribute to the 
      metastatic progression of tumor cells through paracrine signalling. It has been 
      recently discovered that blood circulating exosomes contain distinguishable 
      fractions of free and cell-surface-associated vesicles. We evaluated the 
      influence of protein cargoes from exosomes from plasma, and exosomes from the 
      total blood of healthy females (HFs) and breast cancer patients (BCPs), on cell 
      motility. We conducted a mass spectrometric analysis of exosomal contents 
      isolated from samples using ultrafiltration and ultracentrifugation approaches 
      and verified their nature using transmission electron microscopy, nanoparticle 
      tracking analysis and flow cytometry. We observed that malignant 
      neoplasm-associated proteins in exosomes from BCP total blood were detected more 
      often than in plasma (66% vs. 59%). FunRich analysis to assess Gene Ontology (GO) 
      enrichment revealed that proteins with catalytic activities, transporter 
      functions and protein metabolism activities were increased in exosomes from BCP 
      blood. Finally, GO analysis revealed that proteomic profiles of exosomes from HF 
      total blood were enriched with proteins inhibiting cell migration and invasion, 
      which explains the low stimulating activity of exosomes from HF total blood on 
      SKBR-3 cancer cell migration velocity. This allows exosomes to act as 
      intermediaries providing intercellular communications through horizontal transfer 
      of RNA and functionally active proteins, potentially affecting the development of 
      both primary neoplasms and distant metastases.
FAU - Tutanov, Oleg
AU  - Tutanov O
AD  - Laboratory of Molecular Medicine, Institute of Chemical Biology and Fundamental 
      Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, 
      Russia.
FAU - Orlova, Evgeniya
AU  - Orlova E
AD  - Laboratory of Carcinogenesis Mechanisms, "N.N. Blokhin Cancer Research Center" of 
      the Ministry of Health of the Russian Federation, 115478 Moscow, Russia.
FAU - Proskura, Ksenia
AU  - Proskura K
AD  - Laboratory of Molecular Medicine, Institute of Chemical Biology and Fundamental 
      Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, 
      Russia.
AD  - Department of Mammology, Novosibirsk Regional Clinical Oncological Dispensary, 
      630108 Novosibirsk, Russia.
FAU - Grigor'eva, Alina
AU  - Grigor'eva A
AD  - Laboratory of Molecular Medicine, Institute of Chemical Biology and Fundamental 
      Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, 
      Russia.
FAU - Yunusova, Natalia
AU  - Yunusova N
AD  - Laboratory of Tumor Biochemistry, Cancer Research Institute, capital TE, Cyrillicomsk National 
      Research Medical Center, Russian Academy of Science, 634028 Tomsk, Russia.
AD  - Department of Biochemistry and Molecular Biology, Siberian State Medical 
      University, 634050 Tomsk, Russia.
FAU - Tsentalovich, Yuri
AU  - Tsentalovich Y
AD  - Laboratory of Proteomics and Metabolomics, International Tomography Center SB 
      RAS, 630090 Novosibirsk, Russia.
FAU - Alexandrova, Antonina
AU  - Alexandrova A
AD  - Laboratory of Carcinogenesis Mechanisms, "N.N. Blokhin Cancer Research Center" of 
      the Ministry of Health of the Russian Federation, 115478 Moscow, Russia.
FAU - Tamkovich, Svetlana
AU  - Tamkovich S
AD  - Laboratory of Molecular Medicine, Institute of Chemical Biology and Fundamental 
      Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, 
      Russia.
AD  - Department of Molecular Biology and Biotechnology, Novosibirsk State University, 
      630090 Novosibirsk, Russia.
LA  - eng
GR  - 18-415-540012/capital ER, Cyrillicsmall o, Cyrillicsmall es, Cyrillicsmall es, Cyrillicsmall i, Cyrillicsmall short i, Cyrillicsmall es, Cyrillicsmall ka, Cyrillicsmall i, Cyrillicsmall short i, Cyrillic capital EF, Cyrillicsmall o, Cyrillicsmall en, Cyrillicsmall de, Cyrillic capital EF, Cyrillicsmall u, Cyrillicsmall en, Cyrillicsmall de, Cyrillicsmall a, Cyrillicsmall em, Cyrillicsmall ie, Cyrillicsmall en, Cyrillicsmall te, Cyrillicsmall a, Cyrillicsmall el, Cyrillicsmall soft sign, Cyrillicsmall en, Cyrillicsmall yeru, Cyrillicsmall ha, Cyrillic capital I, Cyrillicsmall es, Cyrillicsmall es, Cyrillicsmall el, Cyrillicsmall ie, Cyrillicsmall de, Cyrillicsmall o, Cyrillicsmall ve, Cyrillicsmall a, Cyrillicsmall en, Cyrillicsmall i, Cyrillicsmall short i, Cyrillic (capital ER, Cyrilliccapital EF, Cyrilliccapital EF, Cyrilliccapital I, Cyrillic)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200325
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (Antigens, CD)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - EC 3.4.- (Amyloid Precursor Protein Secretases)
RN  - EC 3.4.24.81 (ADAM10 Protein)
RN  - EC 3.4.24.81 (ADAM10 protein, human)
SB  - IM
MH  - ADAM10 Protein/blood
MH  - Adult
MH  - Aged
MH  - Amyloid Precursor Protein Secretases/blood
MH  - Antigens, CD/metabolism
MH  - Breast Neoplasms/blood/*pathology
MH  - Cell Line, Tumor
MH  - *Cell Movement
MH  - Cell-Free System
MH  - Epithelial Cells/metabolism/pathology
MH  - Exosomes/*metabolism/transplantation
MH  - Female
MH  - Humans
MH  - Membrane Proteins/blood
MH  - Middle Aged
MH  - Proteins/*analysis/metabolism
MH  - Proteomics/methods
PMC - PMC7226042
OTO - NOTNLM
OT  - breast cancer
OT  - exosomes
OT  - mass-spectrometry
OT  - migration
COIS- The authors declare no conflict of interest.
EDAT- 2020/03/29 06:00
MHDA- 2021/04/16 06:00
PMCR- 2020/04/01
CRDT- 2020/03/29 06:00
PHST- 2020/02/26 00:00 [received]
PHST- 2020/03/23 00:00 [revised]
PHST- 2020/03/24 00:00 [accepted]
PHST- 2020/03/29 06:00 [entrez]
PHST- 2020/03/29 06:00 [pubmed]
PHST- 2021/04/16 06:00 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - biom10040495 [pii]
AID - biomolecules-10-00495 [pii]
AID - 10.3390/biom10040495 [doi]
PST - epublish
SO  - Biomolecules. 2020 Mar 25;10(4):495. doi: 10.3390/biom10040495.