PMID- 32218214
OWN - NLM
STAT- MEDLINE
DCOM- 20210415
LR  - 20240328
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 10
IP  - 4
DP  - 2020 Mar 25
TI  - Mapping the Salt Stress-Induced Changes in the Root miRNome in Pokkali Rice.
LID - 10.3390/biom10040498 [doi]
LID - 498
AB  - A plant's response to stress conditions is governed by intricately coordinated 
      gene expression. The microRNAs (miRs) have emerged as relatively new players in 
      the genetic network, regulating gene expression at the transcriptional and 
      post-transcriptional level. In this study, we performed comprehensive profiling 
      of miRs in roots of the naturally salt-tolerant Pokkali rice variety to 
      understand their role in regulating plant physiology in the presence of salt. For 
      comparisons, root miR profiles of the salt-sensitive rice variety Pusa Basmati 
      were generated. It was seen that the expression levels of 65 miRs were similar 
      for roots of Pokkali grown in the absence of salt (PKNR) and Pusa Basmati grown 
      in the presence of salt (PBSR). The salt-induced dis-regulations in expression 
      profiles of miRs showed controlled changes in the roots of Pokkali (PKSR) as 
      compared to larger variations seen in the roots of Pusa Basmati. Target analysis 
      of salt-deregulated miRs identified key transcription factors, ion-transporters, 
      and signaling molecules that act to maintain cellular Ca(2+) homeostasis and 
      limit ROS production. These miR:mRNA nodes were mapped to the Quantitative trait 
      loci (QTLs) to identify the correlated root traits for understanding their 
      significance in plant physiology. The results obtained indicate that the 
      adaptability of Pokkali to excess salt may be due to the genetic regulation of 
      different cellular components by a variety of miRs.
FAU - Goswami, Kavita
AU  - Goswami K
AD  - Plant RNAi Biology Group, International Centre for Genetic Engineering and 
      Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
AD  - Department of Computational Biology and Bioinformatics, Jacob School of 
      Biotechnology and Bioengineering, Sam Higginbottom university of Agriculture, 
      Technology and Sciences, Prayagraj (Formally Allahabad) 211007, India.
FAU - Mittal, Deepti
AU  - Mittal D
AD  - Plant RNAi Biology Group, International Centre for Genetic Engineering and 
      Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
FAU - Gautam, Budhayash
AU  - Gautam B
AUID- ORCID: 0000-0003-3529-7417
AD  - Department of Computational Biology and Bioinformatics, Jacob School of 
      Biotechnology and Bioengineering, Sam Higginbottom university of Agriculture, 
      Technology and Sciences, Prayagraj (Formally Allahabad) 211007, India.
FAU - Sopory, Sudhir K
AU  - Sopory SK
AD  - Plant RNAi Biology Group, International Centre for Genetic Engineering and 
      Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
FAU - Sanan-Mishra, Neeti
AU  - Sanan-Mishra N
AUID- ORCID: 0000-0002-7986-9969
AD  - Plant RNAi Biology Group, International Centre for Genetic Engineering and 
      Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
LA  - eng
GR  - BT/PR10071/AGR/36/31/2007/Department of Biotechnology , Ministry of Science and 
      Technology/International
GR  - BT/PR628/AGR/36/674/2011/Department of Biotechnology , Ministry of Science and 
      Technology/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200325
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Gene Expression Regulation, Plant
MH  - MicroRNAs/*genetics
MH  - Oryza/*genetics/physiology
MH  - Plant Roots/*genetics/physiology
MH  - Polymerase Chain Reaction/methods
MH  - Quantitative Trait Loci
MH  - Reproducibility of Results
MH  - Salt Stress/*genetics
MH  - Salt Tolerance/genetics
MH  - Salt-Tolerant Plants/genetics
PMC - PMC7226372
OTO - NOTNLM
OT  - Pokkali root
OT  - degradome
OT  - expression profiles
OT  - miR-eQTL
OT  - microRNA
OT  - salt tolerance
COIS- The authors declare no competing financial interests.
EDAT- 2020/03/29 06:00
MHDA- 2021/04/16 06:00
PMCR- 2020/04/01
CRDT- 2020/03/29 06:00
PHST- 2020/01/09 00:00 [received]
PHST- 2020/03/05 00:00 [revised]
PHST- 2020/03/06 00:00 [accepted]
PHST- 2020/03/29 06:00 [entrez]
PHST- 2020/03/29 06:00 [pubmed]
PHST- 2021/04/16 06:00 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - biom10040498 [pii]
AID - biomolecules-10-00498 [pii]
AID - 10.3390/biom10040498 [doi]
PST - epublish
SO  - Biomolecules. 2020 Mar 25;10(4):498. doi: 10.3390/biom10040498.