PMID- 32218368
OWN - NLM
STAT- MEDLINE
DCOM- 20201221
LR  - 20201221
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 18
IP  - 4
DP  - 2020 Mar 25
TI  - Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen 
      Hydrogels for Wound Dressings.
LID - 10.3390/md18040178 [doi]
LID - 178
AB  - Collagen plays an important role in the formation of extracellular matrix (ECM) 
      and development/migration of cells and tissues. Here we report the preparation of 
      collagen and collagen hydrogel from the skin of tilapia and an evaluation of 
      their potential as a wound dressing for the treatment of refractory wounds. The 
      acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted and 
      characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis 
      (SDS-PAGE), differential scanning calorimetry (DSC), circular dichroism (CD) and 
      Fourier transform infrared spectroscopy (FTIR) analysis. Both ASC and PSC belong 
      to type I collagen and have a complete triple helix structure, but PSC shows 
      lower molecular weight and thermal stability, and has the inherent low 
      antigenicity. Therefore, PSC was selected to prepare biomedical hydrogels using 
      its self-aggregating properties. Rheological characterization showed that the 
      mechanical strength of the hydrogels increased as the PSC content increased. 
      Scanning electron microscope (SEM) analysis indicated that hydrogels could form a 
      regular network structure at a suitable PSC content. Cytotoxicity experiments 
      confirmed that hydrogels with different PSC content showed no significant 
      toxicity to fibroblasts. Skin repair experiments and pathological analysis showed 
      that the collagen hydrogels wound dressing could significantly accelerate the 
      healing of deep second-degree burn wounds and the generation of new skin 
      appendages, which can be used for treatment of various refractory wounds.
FAU - Ge, Baosheng
AU  - Ge B
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao 266580, China.
FAU - Wang, Haonan
AU  - Wang H
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao 266580, China.
FAU - Li, Jie
AU  - Li J
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao 266580, China.
AD  - Yantai Institute of Coastal Zone, Chinese Academy of Sciences, Yantai, 264003, 
      China.
FAU - Liu, Hengheng
AU  - Liu H
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao 266580, China.
FAU - Yin, Yonghao
AU  - Yin Y
AD  - State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and 
      Biotechnology, China University of Petroleum (East China), Qingdao 266580, China.
FAU - Zhang, Naili
AU  - Zhang N
AD  - Department of Human Anatomy, School of Basic Medical Science, Binzhou Medical 
      University, 346 Guanhai Road, Laishan, Yantai, 264003, China.
FAU - Qin, Song
AU  - Qin S
AD  - Yantai Institute of Coastal Zone, Chinese Academy of Sciences, Yantai, 264003, 
      China.
LA  - eng
GR  - 21673294/National Natural Science Foundation of China/
GR  - ZR2019ZD17/Natural Science Foundation of Shandong Province/
GR  - J18KA258/Shandong Province Higher Educational Science and Technology Program/
GR  - BS2015SW021/Natural Science Foundation of Shandong Province/
PT  - Journal Article
DEP - 20200325
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (Collagen Type I)
RN  - 0 (Fish Proteins)
RN  - 0 (Hydrogels)
SB  - IM
MH  - Animals
MH  - *Bandages
MH  - Burns/*therapy
MH  - *Cichlids
MH  - Collagen Type I/isolation & purification/*pharmacology/ultrastructure
MH  - Disease Models, Animal
MH  - Female
MH  - Fish Proteins/isolation & purification/*pharmacology/ultrastructure
MH  - Humans
MH  - Hydrogels/pharmacology
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Rats
MH  - Skin/chemistry/injuries
MH  - Wound Healing/drug effects
PMC - PMC7230254
OTO - NOTNLM
OT  - characterization
OT  - collagen hydrogel
OT  - fish skin
OT  - wound dressing
COIS- The authors declare no conflict of interest.
EDAT- 2020/03/29 06:00
MHDA- 2020/12/22 06:00
PMCR- 2020/04/01
CRDT- 2020/03/29 06:00
PHST- 2020/02/24 00:00 [received]
PHST- 2020/03/17 00:00 [revised]
PHST- 2020/03/23 00:00 [accepted]
PHST- 2020/03/29 06:00 [entrez]
PHST- 2020/03/29 06:00 [pubmed]
PHST- 2020/12/22 06:00 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - md18040178 [pii]
AID - marinedrugs-18-00178 [pii]
AID - 10.3390/md18040178 [doi]
PST - epublish
SO  - Mar Drugs. 2020 Mar 25;18(4):178. doi: 10.3390/md18040178.