PMID- 32218735
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 11
DP  - 2020
TI  - Neuroprotective Effect of Danhong Injection on Cerebral Ischemia-Reperfusion 
      Injury in Rats by Activation of the PI3K-Akt Pathway.
PG  - 298
LID - 10.3389/fphar.2020.00298 [doi]
LID - 298
AB  - Many traditional Chinese medicines, including Danhong injection (DHI), can be 
      used to treat cerebral ischemia-reperfusion injury and have neuroprotective 
      effects on the brain; however, few studies have explored the mechanism by which 
      this effect is generated. In this study, we investigated the neuroprotective 
      effect of DHI against cerebral ischemia-reperfusion injury mediated via the 
      PI3K-Akt signaling pathway. After establishing the model of middle cerebral 
      artery occlusion (MCAO), 60 male Sprague-Dawley rats were allocated to six groups 
      as follows: sham, MCAO, DHI (MCAO + DHI), LY294002 (MCAO + LY294002 [PI3K-Akt 
      pathway specific inhibitor]), DHI + LY294002 (MCAO + DHI + LY294002), and NMDP + 
      LY294002 (MCAO + NMDP [nimodipine] + LY294002). Hematoxylin and eosin (HE) and 
      terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining 
      were used to evaluate the pathological changes of brain tissue and the degree of 
      neuronal apoptosis. Real-time quantitative polymerase chain reaction (qRT-PCR), 
      western blot analysis and enzyme-linked immunosorbent assays were used to measure 
      the expression of Bad, Bax, Bcl-2, Bim, P53, MDM2, Akt, PI3K, p-Akt, p-PI3K, and 
      Cyt-C. Compared with the MCAO group, brain tissue cell apoptosis was 
      significantly reduced in the DHI group, and the brain function score was 
      significantly improved. In addition, the expression of pro-apoptotic factors 
      (Bad, Bax, and Bim) was significantly downregulated in the DHI group, while 
      expression of the anti-apoptotic factor Bcl-2 was significantly upregulated, and 
      expression of the apoptotic gene p53 was also significantly attenuated. Moreover, 
      this neuroprotective effect was attenuated by the PI3K-Akt signaling pathway 
      inhibitor (LY294002). Thus, our results confirmed the neuroprotective effects of 
      DHI in rats with ischemia-reperfusion injury and indicate that these effects on 
      the brain are partly generated by activation of the PI3K-Akt signaling pathway.
CI  - Copyright (c) 2020 Feng, Wan, Zhang, Yu, Shao, He, Wan and Jin.
FAU - Feng, Chen
AU  - Feng C
AD  - The Second Clinical Medical College, Zhejiang Chinese Medical University, 
      Hangzhou, China.
FAU - Wan, Haofang
AU  - Wan H
AD  - Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, 
      Hangzhou, China.
FAU - Zhang, Yangyang
AU  - Zhang Y
AD  - College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Yu, Li
AU  - Yu L
AD  - College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Shao, Chongyu
AU  - Shao C
AD  - College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - He, Yu
AU  - He Y
AD  - College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, 
      Hangzhou, China.
FAU - Wan, Haitong
AU  - Wan H
AD  - College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Jin, Weifeng
AU  - Jin W
AD  - College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, 
      Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20200311
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC7078680
OTO - NOTNLM
OT  - Danhong injection
OT  - PI3K-Akt pathway
OT  - apoptosis
OT  - ischemia-reperfusion
OT  - neuroprotection
EDAT- 2020/03/29 06:00
MHDA- 2020/03/29 06:01
PMCR- 2020/03/11
CRDT- 2020/03/29 06:00
PHST- 2019/11/19 00:00 [received]
PHST- 2020/02/27 00:00 [accepted]
PHST- 2020/03/29 06:00 [entrez]
PHST- 2020/03/29 06:00 [pubmed]
PHST- 2020/03/29 06:01 [medline]
PHST- 2020/03/11 00:00 [pmc-release]
AID - 10.3389/fphar.2020.00298 [doi]
PST - epublish
SO  - Front Pharmacol. 2020 Mar 11;11:298. doi: 10.3389/fphar.2020.00298. eCollection 
      2020.