PMID- 32218850
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 19
IP  - 4
DP  - 2020 Apr
TI  - Rapamycin inhibits proliferation and apoptosis of retinoblastoma cells through 
      PI3K/AKT signaling pathway.
PG  - 2950-2956
LID - 10.3892/ol.2020.11363 [doi]
AB  - Effects of Rapamycin on the proliferation and apoptosis of retinoblastoma cells 
      through the phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (AKT) 
      signaling pathway were studied. The retinoblastoma Y79 cells were selected and 
      divided into negative control group (NC group), 0.2 microM Rapamycin group and 0.4 microM 
      Rapamycin group. Then the proliferative activity of Y79 cells was detected using 
      Cell Counting Kit-8 (CCK8) assay, the content of reactive oxygen species (ROS), 
      malondialdehyde (MDA) and superoxide dismutase (SOD) in cells in each group was 
      detected using enzyme-linked immunosorbent assay (ELISA), and the apoptosis of 
      Y79 cells was detected via terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) assay. Moreover, the changes in Y79 cell cycle and 
      apoptosis were determined through flow cytometry, and apoptosis and PI3K/AKT 
      pathway were detected using reverse transcription-polymerase chain reaction 
      (RT-PCR) and western blotting. It was found that the number of cells and the 
      proliferative activity were significantly reduced in 0.2 microM Rapamycin group and 
      0.4 microM Rapamycin group. In 0.2 microM Rapamycin group and 0.4 microM Rapamycin group, the 
      content of ROS and MDA was significantly decreased, while that of SOD was notably 
      increased. TUNEL assay and flow cytometry showed that in 0.2 microM Rapamycin group 
      and 0.4 microM Rapamycin group, the number of apoptotic cells was obviously 
      increased, and the cell cycle was basically arrested in S phase. The expression 
      levels of Bcl-2, PI3K and AKT declined in 0.2 microM Rapamycin group and 0.4 microM 
      Rapamycin group, whereas the expression of Caspase 8 increased. Similar results 
      were also obtained in the protein assay. The above results were significantly 
      superior in 0.4 microM Rapamycin group to those in 0.2 microM Rapamycin group. Rapamycin 
      inhibits proliferation and promotes apoptosis of retinoblastoma cells through 
      inhibiting the PI3K/AKT signaling pathway.
CI  - Copyright: (c) Yao et al.
FAU - Yao, Jun
AU  - Yao J
AD  - Department of Ophthalmology, The Central Hospital of Wuhan, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. 
      China.
FAU - Xu, Min
AU  - Xu M
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Xian Jiaotong University, Xian, Shaanxi 710004, P.R. China.
FAU - Liu, Ziyao
AU  - Liu Z
AD  - Department of Ophthalmology, The Second Affiliated Hospital of Xian Jiaotong 
      University, Xian, Shaanxi 710004, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200130
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC7068238
OTO - NOTNLM
OT  - PI3K/AKT signaling pathway
OT  - Rapamycin
OT  - apoptosis
OT  - proliferation
OT  - retinoblastoma cells
EDAT- 2020/03/29 06:00
MHDA- 2020/03/29 06:01
PMCR- 2020/01/30
CRDT- 2020/03/29 06:00
PHST- 2019/08/26 00:00 [received]
PHST- 2019/12/03 00:00 [accepted]
PHST- 2020/03/29 06:00 [entrez]
PHST- 2020/03/29 06:00 [pubmed]
PHST- 2020/03/29 06:01 [medline]
PHST- 2020/01/30 00:00 [pmc-release]
AID - OL-0-0-11363 [pii]
AID - 10.3892/ol.2020.11363 [doi]
PST - ppublish
SO  - Oncol Lett. 2020 Apr;19(4):2950-2956. doi: 10.3892/ol.2020.11363. Epub 2020 Jan 
      30.