PMID- 32219602
OWN - NLM
STAT- MEDLINE
DCOM- 20210608
LR  - 20210608
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 28
IP  - 2
DP  - 2020 Jun
TI  - A universal chromosome identification system for maize and wild Zea species.
PG  - 183-194
LID - 10.1007/s10577-020-09630-5 [doi]
AB  - Maize was one of the first eukaryotic species in which individual chromosomes can 
      be identified cytologically, which made maize one of the oldest models for 
      genetics and cytogenetics research. Nevertheless, consistent identification of 
      all 10 chromosomes from different maize lines as well as from wild Zea species 
      remains a challenge. We developed a new technique for maize chromosome 
      identification based on fluorescence in situ hybridization (FISH). We developed 
      two oligonucleotide-based probes that hybridize to 24 chromosomal regions. 
      Individual maize chromosomes show distinct FISH signal patterns, which allow 
      universal identification of all chromosomes from different Zea species. We 
      developed karyotypes from three Zea mays subspecies and two additional wild Zea 
      species based on individually identified chromosomes. A paracentric inversion was 
      discovered on the long arm of chromosome 4 in Z. nicaraguensis and Z. luxurians 
      based on modifications of the FISH signal patterns. Chromosomes from these two 
      species also showed distinct distribution patterns of terminal knobs compared 
      with other Zea species. These results support that Z. nicaraguensis and Z. 
      luxurians are closely related species.
FAU - Braz, Guilherme T
AU  - Braz GT
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA.
FAU - do Vale Martins, Livia
AU  - do Vale Martins L
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of 
      Plant Functional Genomics of Ministry of Education, Yangzhou University, 
      Yangzhou, 225009, China.
FAU - Albert, Patrice S
AU  - Albert PS
AD  - Division of Biological Sciences, University of Missouri, Columbia, MO, 65211, 
      USA.
FAU - Birchler, James A
AU  - Birchler JA
AD  - Division of Biological Sciences, University of Missouri, Columbia, MO, 65211, 
      USA.
FAU - Jiang, Jiming
AU  - Jiang J
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA. jiangjm@msu.edu.
AD  - Department of Horticulture, Michigan State University, East Lansing, MI, 48824, 
      USA. jiangjm@msu.edu.
AD  - AgBioResearch, Michigan State University, East Lansing, MI, 48824, USA. 
      jiangjm@msu.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200326
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Chromosome Inversion
MH  - *Chromosomes, Plant
MH  - *Cytogenetics/methods
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotyping/methods
MH  - Oligonucleotide Probes
MH  - Zea mays/*classification/*genetics
OTO - NOTNLM
OT  - Chromosome identification
OT  - FISH
OT  - Karyotype
OT  - Maize
OT  - Oligo-FISH
EDAT- 2020/03/29 06:00
MHDA- 2021/06/09 06:00
CRDT- 2020/03/29 06:00
PHST- 2020/02/04 00:00 [received]
PHST- 2020/03/17 00:00 [accepted]
PHST- 2020/03/16 00:00 [revised]
PHST- 2020/03/29 06:00 [pubmed]
PHST- 2021/06/09 06:00 [medline]
PHST- 2020/03/29 06:00 [entrez]
AID - 10.1007/s10577-020-09630-5 [pii]
AID - 10.1007/s10577-020-09630-5 [doi]
PST - ppublish
SO  - Chromosome Res. 2020 Jun;28(2):183-194. doi: 10.1007/s10577-020-09630-5. Epub 
      2020 Mar 26.