PMID- 32220059
OWN - NLM
STAT- MEDLINE
DCOM- 20210511
LR  - 20230330
IS  - 2050-4527 (Electronic)
IS  - 2050-4527 (Linking)
VI  - 8
IP  - 2
DP  - 2020 Jun
TI  - Peanut applied to the skin of nonhuman primates induces antigen-specific IgG but 
      not IgE.
PG  - 211-215
LID - 10.1002/iid3.296 [doi]
AB  - INTRODUCTION: Previous studies in humans support the dual-allergen exposure 
      hypothesis, and several studies in mouse models have demonstrated that cutaneous 
      exposure to disrupted or intact skin can lead to sensitization to peanut. 
      However, the field lacks definitive evidence that cutaneous exposure leads to 
      peanut allergy in humans or other primates. METHODS: Peanut extract was applied 
      to the shaved back of the neck of four male and four female African green monkeys 
      three times per week for 4 weeks. An oral food challenge (OFC) was performed the 
      following week by gavage of 200 mg of peanut protein, and vital signs were 
      monitored for 30 minutes post-OFC. Blood was collected at baseline, day 11, day 
      32, and 30 minutes post-OFC. Total IgE, and peanut-specific immunoglobulin E 
      (IgE) and immunoglobulin G (IgG) were quantified in serum collected throughout 
      the 4 weeks. Histamine was measured in serum collected 30 minutes post-OFC. 
      RESULTS: Peanut-specific IgE was undetectable at any time points in any of the 
      monkeys, and there was no consistent increase in total IgE. During the oral 
      challenge, none of the monkeys experienced allergic symptoms and histamine levels 
      did not change. However, seven of the eight monkeys produced increasing 
      peanut-specific IgG by day 32, indicating that repeated skin exposure to peanut 
      is immunogenic. CONCLUSIONS: Skin exposure to peanut did not lead to 
      sensitization in this study, and monkeys did not experience anaphylaxis upon 
      peanut challenge. However, monkeys produced increased peanut-specific IgG 
      throughout peanut exposure, indicating that repeated skin exposure to peanut is 
      immunogenic.
CI  - (c) 2020 The Authors. Immunity, Inflammation and Disease published by John Wiley & 
      Sons Ltd.
FAU - Kulis, Michael D
AU  - Kulis MD
AD  - Division of Allergy, Immunology, and Rheumatology, Department of Pediatrics, 
      University of North Carolina, Chapel Hill, North Carolina.
AD  - Department of Pediatrics, University of North Carolina Food Allergy Initiative, 
      University of North Carolina, Chapel Hill, North Carolina.
FAU - Smeekens, Johanna M
AU  - Smeekens JM
AUID- ORCID: 0000-0001-9651-3852
AD  - Division of Allergy, Immunology, and Rheumatology, Department of Pediatrics, 
      University of North Carolina, Chapel Hill, North Carolina.
AD  - Department of Pediatrics, University of North Carolina Food Allergy Initiative, 
      University of North Carolina, Chapel Hill, North Carolina.
FAU - Kavanagh, Kylie
AU  - Kavanagh K
AD  - Department of Pathology, Section on Comparative Medicine, Wake Forest School of 
      Medicine, Winston-Salem, North Carolina.
AD  - Department of Biomedicine, University of Tasmania, Hobart, Tasmania, Australia.
FAU - Jorgensen, Matthew J
AU  - Jorgensen MJ
AD  - Department of Pathology, Section on Comparative Medicine, Wake Forest School of 
      Medicine, Winston-Salem, North Carolina.
LA  - eng
GR  - P30 ES010126/ES/NIEHS NIH HHS/United States
GR  - P40 OD010965/OD/NIH HHS/United States
GR  - UL1 TR001420/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20200327
PL  - England
TA  - Immun Inflamm Dis
JT  - Immunity, inflammation and disease
JID - 101635460
RN  - 0 (Allergens)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Allergens/*immunology
MH  - Anaphylaxis/immunology
MH  - Animals
MH  - Arachis/immunology
MH  - Chlorocebus aethiops
MH  - Disease Models, Animal
MH  - Female
MH  - Histamine/blood
MH  - Immunoglobulin E/*blood
MH  - Immunoglobulin G/*blood
MH  - Male
MH  - Peanut Hypersensitivity/blood/*immunology
MH  - Skin/immunology
MH  - Time Factors
PMC - PMC7212195
OTO - NOTNLM
OT  - African green monkey
OT  - IgE
OT  - IgG
OT  - cutaneous
OT  - food allergy
OT  - nonhuman primate
OT  - peanut allergy
COIS- The authors declare that there are no conflict of interests.
EDAT- 2020/03/29 06:00
MHDA- 2021/05/12 06:00
PMCR- 2020/03/27
CRDT- 2020/03/29 06:00
PHST- 2019/09/26 00:00 [received]
PHST- 2020/01/23 00:00 [revised]
PHST- 2020/02/25 00:00 [accepted]
PHST- 2020/03/29 06:00 [pubmed]
PHST- 2021/05/12 06:00 [medline]
PHST- 2020/03/29 06:00 [entrez]
PHST- 2020/03/27 00:00 [pmc-release]
AID - IID3296 [pii]
AID - 10.1002/iid3.296 [doi]
PST - ppublish
SO  - Immun Inflamm Dis. 2020 Jun;8(2):211-215. doi: 10.1002/iid3.296. Epub 2020 Mar 
      27.