PMID- 32223956
OWN - NLM
STAT- MEDLINE
DCOM- 20201113
LR  - 20201113
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 63
DP  - 2020 Apr
TI  - Nrf2 played an important role in radiation protection effect of low-level laser 
      exposed on umbilical cord mesenchymal stem cell.
PG  - 101329
LID - S0040-8166(19)30367-2 [pii]
LID - 10.1016/j.tice.2019.101329 [doi]
AB  - To investigate the protective function of low-level laser irradiation (LLLI) 
      against ionizing irradiation and explore the molecular mechanism of 
      photomodulation of Nrf2 protein, the impact of LLLI (635 nm, 5.7 J/cm(2)) before 
      2 Gy gamma ray radiation of radio-sensitive tissue hematopoietic stem cells was 
      evaluated. As a result, reduced levels of reactive oxygen species and increased 
      expression of antioxidant enzymes were detected. Moreover, increased expression 
      of Nrf2 was observed after LLLI, whereas brusatol pretreatment before LLLI 
      abolished this effect. In vivo, transplantation of human umbilical cord 
      mesenchymal stem cells (hUC-MSCs) was employed for therapy of hematopoietic 
      function in an acute radiation sickness (H-ARS) mouse model, which was induced by 
      6-Gy ionizing irradiation; different hUC-MSC pretreatments including LLLI and 
      Nrf2 RNAi were accounted for during experimental grouping. LLLI treatment of 
      cells significantly increased the erythrocyte count and number of myelopoiesis 
      clones (P < 0.05), but such improvements were reduced by Nrf2 RNAi pretreatment 
      compared with cells transplanted without intervention. Therefore, LLLI may 
      improve the radiation protection effect through molecular mechanisms related to 
      the Nrf2 antioxidant pathway.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Wang, Hong
AU  - Wang H
AD  - Geriatric Health Engineering Research Center, Institute of Biomedical 
      Engineering, Chinese Academy of Medical Science & Peking Union Medical College, 
      Tianjin, 300192, China.
FAU - Tu, Wen-Jun
AU  - Tu WJ
AD  - Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, 300192, China.
FAU - Xiao, Changyan
AU  - Xiao C
AD  - Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, 300192, China.
FAU - Dong, Ming-Xin
AU  - Dong MX
AD  - Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, 300192, China.
FAU - Ye, Yin-Tao
AU  - Ye YT
AD  - Cancer Institute & Hospital, Tianjin Medical University, Tianjin, 300060, China.
FAU - Deng, Juan
AU  - Deng J
AD  - Geriatric Health Engineering Research Center, Institute of Biomedical 
      Engineering, Chinese Academy of Medical Science & Peking Union Medical College, 
      Tianjin, 300192, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Geriatric Health Engineering Research Center, Institute of Biomedical 
      Engineering, Chinese Academy of Medical Science & Peking Union Medical College, 
      Tianjin, 300192, China.
FAU - Sha, Hong
AU  - Sha H
AD  - Geriatric Health Engineering Research Center, Institute of Biomedical 
      Engineering, Chinese Academy of Medical Science & Peking Union Medical College, 
      Tianjin, 300192, China. Electronic address: shahong2000@163.com.
FAU - Liu, Qiang
AU  - Liu Q
AD  - Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, 300192, China. Electronic address: 
      liuqiang@irm-cams.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20200102
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Nfe2l2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Cell Differentiation/radiation effects
MH  - Cell Line
MH  - Cell Proliferation/*radiation effects
MH  - Gene Expression Regulation/radiation effects
MH  - Humans
MH  - Lasers/adverse effects
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*radiation effects
MH  - Mice
MH  - NF-E2-Related Factor 2/*genetics
MH  - Radiation Protection
MH  - Umbilical Cord/radiation effects
OTO - NOTNLM
OT  - LLLI
OT  - Nrf2
OT  - Radiation protection
EDAT- 2020/04/01 06:00
MHDA- 2020/11/18 06:00
CRDT- 2020/04/01 06:00
PHST- 2019/08/29 00:00 [received]
PHST- 2019/12/31 00:00 [revised]
PHST- 2019/12/31 00:00 [accepted]
PHST- 2020/04/01 06:00 [entrez]
PHST- 2020/04/01 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
AID - S0040-8166(19)30367-2 [pii]
AID - 10.1016/j.tice.2019.101329 [doi]
PST - ppublish
SO  - Tissue Cell. 2020 Apr;63:101329. doi: 10.1016/j.tice.2019.101329. Epub 2020 Jan 
      2.