PMID- 32226262
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1005-9040 (Print)
IS  - 1005-9040 (Linking)
VI  - 29
IP  - 2
DP  - 2013
TI  - Identification of porcine reproductive and respiratory syndrome virus inhibitors 
      through an oriented screening on natural products.
PG  - 290-293
LID - 10.1007/s40242-013-2300-y [doi]
AB  - Porcine reproductive and respiratory syndrome(PRRS) caused by porcine 
      reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious 
      diseases in the swine industry worldwide, causing big economic losses. Vaccines 
      are major weapons against PRRSV, however, current available vaccines have several 
      limitations. Developing chemical drugs as alternatives is required. On the basis 
      of traditional medical knowledge, we purposely selected 15 natural products 
      originated from Chinese herbs with anti-infectious effects. Their antiviral 
      activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 
      cells and reverse transcription polymerase chain reaction(RT-PCR) assay. 
      Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits 
      which could significantly reduce PRRSV-associated CPE with 50% inhibited 
      concentration(IC(50)) values of 7.9 and 39.4 mumol/L, respectively. Meanwhile, 
      compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA 
      expression of ORF7 gene in a dose-dependent manner. Study results suggest that 
      compounds ethoxysanguinarine and atractylodinol may be useful anti-PRRSV drugs 
      for swine industry or the hits for further lead optimization.
CI  - (c) Jilin University, The Editorial Department of Chemical Research in Chinese 
      Universities and Springer-Verlag GmbH 2013.
FAU - Li, Wen-Gui
AU  - Li WG
AD  - 12300College of Animal Science and Technology, Yunnan Agricultural University, 
      Kunming, 650201 P. R. China. GRID: grid.410696.c
FAU - Dai, Fei-Yan
AU  - Dai FY
AD  - 12300College of Animal Science and Technology, Yunnan Agricultural University, 
      Kunming, 650201 P. R. China. GRID: grid.410696.c
FAU - Cheng, Yong-Xian
AU  - Cheng YX
AD  - 22300State Key Laboratory of Phytochemistry and Plant Resources in West China, 
      Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204 P. R. 
      China. GRID: grid.9227.e. ISNI: 0000000119573309
FAU - Yin, Ge-Fen
AU  - Yin GF
AD  - 12300College of Animal Science and Technology, Yunnan Agricultural University, 
      Kunming, 650201 P. R. China. GRID: grid.410696.c
FAU - Bi, Jun-Long
AU  - Bi JL
AD  - 12300College of Animal Science and Technology, Yunnan Agricultural University, 
      Kunming, 650201 P. R. China. GRID: grid.410696.c
FAU - Li, Dian-Peng
AU  - Li DP
AD  - 32300Guangxi Key Laboratory of Functional Phytochemicals Research and 
      Utilization, Guangxi Institute of Botany, Guilin, 541006 P. R. China. GRID: 
      grid.469559.2. ISNI: 0000000096772830
LA  - eng
PT  - Journal Article
DEP - 20130310
PL  - China (Republic : 1949- )
TA  - Chem Res Chin Univ
JT  - Chemical research in Chinese universities
JID - 101279493
PMC - PMC7099335
OTO - NOTNLM
OT  - Antiviral activity
OT  - Cytopathic effect
OT  - Natural product
OT  - Porcine reproductive and respiratory syndrome virus(PRRSV)
EDAT- 2013/01/01 00:00
MHDA- 2013/01/01 00:01
PMCR- 2020/03/27
CRDT- 2020/04/01 06:00
PHST- 2012/08/13 00:00 [received]
PHST- 2012/10/15 00:00 [accepted]
PHST- 2020/04/01 06:00 [entrez]
PHST- 2013/01/01 00:00 [pubmed]
PHST- 2013/01/01 00:01 [medline]
PHST- 2020/03/27 00:00 [pmc-release]
AID - 2300 [pii]
AID - 10.1007/s40242-013-2300-y [doi]
PST - ppublish
SO  - Chem Res Chin Univ. 2013;29(2):290-293. doi: 10.1007/s40242-013-2300-y. Epub 2013 
      Mar 10.