PMID- 32232612
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210402
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 72
IP  - 2
DP  - 2020 Apr
TI  - Application of improved single blastomere fixation technique in preimplantation 
      genetic diagnosis.
PG  - 217-226
LID - 10.1007/s10616-020-00371-1 [doi]
AB  - To modify a fixation method improving the intensity and clarity of the single 
      blastomeric signal detection by fluorescence in situ hybridization (FISH) in 
      preimplantation genetic diagnosis. 333 cycles of assisted reproduction with 
      preimplantation genetic diagnosis FISH (PGD-FISH) performed in our hospital were 
      analyzed and a total of 3452 single blastomeres were obtained. For the 
      conventional fixation method, the blastomeres were kept in 0.1% sodium citrate 
      with 0.2 mg/ml bovine serum albumin (BSA) for 2-5 min. FISH was performed and the 
      internal relationship between embryo quality and fixed rate, signal detection 
      rate, and signal determination rate was explored. With the modified method, 
      91.54% of blastomeres were fixed, while 88.30% were fixed with the conventional 
      method. The signal detection rate was significantly increased for the modified 
      group than for the conventional group (compared 98.53% with 94.78%, P < 0.001). 
      Especially, the signal determination rate also showed a significant difference 
      between the two methods (compared 90.51% with 74.17%, P < 0.001). After the 
      development of the fixation method, the fixation efficiency and the signal 
      determination rate were greatly improved, providing more definite diagnosis for 
      the patient. It will hopefully allow more assisted reproduction programs to offer 
      their patients preimplantation genetic diagnosis with FISH.
FAU - Yu, Guanling
AU  - Yu G
AD  - Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, 
      Jinan, 250021, Shandong, China.
FAU - Ma, Shuiying
AU  - Ma S
AD  - Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, 
      Jinan, 250021, Shandong, China.
FAU - Zhu, Yueting
AU  - Zhu Y
AD  - Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, 
      Jinan, 250021, Shandong, China.
FAU - Liu, Yujin
AU  - Liu Y
AD  - Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, 
      Jinan, 250021, Shandong, China.
FAU - Zhang, Haozhen
AU  - Zhang H
AD  - Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, 
      Jinan, 250021, Shandong, China.
FAU - Wu, Keliang
AU  - Wu K
AD  - Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, 
      Jinan, 250021, Shandong, China. wukeliang_527@163.com.
FAU - Hao, Aijun
AU  - Hao A
AD  - Center for Reproductive Medicine, Shandong University, No. 157, Jingliu Road, 
      Jinan, 250021, Shandong, China. aijunhao@sdu.edu.cn.
AD  - Department of Histology and Embryology, Shandong University School of Medicine, 
      No. 44, Wenhua Xi Road, Jinan, 250012, Shandong, China. aijunhao@sdu.edu.cn.
LA  - eng
GR  - 2017YFC1001600/The National Key Research and Development Program of China/
GR  - 81871168/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200331
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC7192997
OTO - NOTNLM
OT  - Blastomere
OT  - Diagnosis
OT  - FISH
OT  - Fixation
OT  - Preimplantation
COIS- The authors declare that there is no conflict of interests.
EDAT- 2020/04/02 06:00
MHDA- 2020/04/02 06:01
PMCR- 2021/04/01
CRDT- 2020/04/02 06:00
PHST- 2019/07/29 00:00 [received]
PHST- 2020/01/09 00:00 [accepted]
PHST- 2020/04/02 06:00 [pubmed]
PHST- 2020/04/02 06:01 [medline]
PHST- 2020/04/02 06:00 [entrez]
PHST- 2021/04/01 00:00 [pmc-release]
AID - 10.1007/s10616-020-00371-1 [pii]
AID - 371 [pii]
AID - 10.1007/s10616-020-00371-1 [doi]
PST - ppublish
SO  - Cytotechnology. 2020 Apr;72(2):217-226. doi: 10.1007/s10616-020-00371-1. Epub 
      2020 Mar 31.