PMID- 32236242
OWN - NLM
STAT- MEDLINE
DCOM- 20210108
LR  - 20210108
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 145
IP  - 9
DP  - 2020 May 7
TI  - A traceless clean-up method coupled with gas chromatography and mass spectrometry 
      for analyzing polycyclic aromatic hydrocarbons in complex plant leaf matrices.
PG  - 3266-3273
LID - 10.1039/d0an00128g [doi]
AB  - This study developed a traceless clean-up method by combining solid phase 
      extraction (SPE) with gas purge-microsyringe extraction (GP-MSE) to purify sample 
      extracts for the determination of polycyclic aromatic hydrocarbons (PAHs) in 
      plant leaves. SPE exhibited good purification performance for the removal of 
      polar lipids, while the GP-MSE technique effectively eliminated less-volatile 
      lipids hence realizing zero damage to the instrument, and significantly improved 
      the peak tailings. After ultrasonic extraction, the combined two-step clean-up 
      procedure successfully removed over 99% of lipids from nineteen types of tree 
      leaves, and PAHs in tree leaves were determined by GC-MS. The relative standard 
      deviations (RSDs) for intra-day (n = 3) and inter-day (n = 3) analyses of PAHs in 
      spiked willow samples were in the range of 0.8%-12.1% and 4.7%-15.3%, 
      respectively. The recoveries of PAHs from spiked willow extracts ranged from 74 
      to 90%, with an average of 86%. The method detection limit (MDL) of PAHs in tree 
      leaves ranged from 0.1 to 4.9 ng g(-1) dry weight. In conclusion, the clean-up 
      method in this study realized the analysis of PAHs in plant leaves with high 
      accuracy, sensitivity and reproducibility. Most importantly, the two-step 
      purification method significantly minimizes damage to the GC-MS system 
      particularly to the column and ion source, which is beneficial to ensure 
      continuous analysis of a large number of samples with good performance.
FAU - Jin, Xiangzi
AU  - Jin X
AD  - Department of Chemistry, Key Laboratory of Biological Resources of Changbai 
      Mountain & Functional Molecules (Yanbian University), Ministry of Education, Park 
      Road 977, Yanji City, Jilin Province 133002, China. drjin@ybu.edu.cn 
      hemiao@ybu.edu.cn.
FAU - Kaw, Han Yeong
AU  - Kaw HY
FAU - Li, Huijie
AU  - Li H
FAU - Zhao Wang
AU  - Zhao Wang
FAU - Zhao, Jinhua
AU  - Zhao J
FAU - Piao, Xiangfan
AU  - Piao X
FAU - Li, Donghao
AU  - Li D
FAU - Jin, Dongri
AU  - Jin D
FAU - He, Miao
AU  - He M
LA  - eng
PT  - Journal Article
DEP - 20200401
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Lipids)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
SB  - IM
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Limit of Detection
MH  - Lipids/isolation & purification
MH  - Pinus/chemistry/metabolism
MH  - Plant Leaves/*chemistry/metabolism
MH  - Polycyclic Aromatic Hydrocarbons/*analysis/isolation & purification
MH  - Quercus/chemistry/metabolism
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/methods
MH  - Sonication
EDAT- 2020/04/03 06:00
MHDA- 2021/01/09 06:00
CRDT- 2020/04/03 06:00
PHST- 2020/04/03 06:00 [pubmed]
PHST- 2021/01/09 06:00 [medline]
PHST- 2020/04/03 06:00 [entrez]
AID - 10.1039/d0an00128g [doi]
PST - ppublish
SO  - Analyst. 2020 May 7;145(9):3266-3273. doi: 10.1039/d0an00128g. Epub 2020 Apr 1.