PMID- 32240742
OWN - NLM
STAT- MEDLINE
DCOM- 20210312
LR  - 20210312
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 155
DP  - 2020 Jul 15
TI  - Purification, structural elucidation and physicochemical properties of a 
      polysaccharide from Abelmoschus esculentus L (okra) flowers.
PG  - 740-750
LID - S0141-8130(20)32822-1 [pii]
LID - 10.1016/j.ijbiomac.2020.03.235 [doi]
AB  - Abelmoschus esculentus L (okra) is widely used as a healthy vegetable and 
      favourable source of dietary medicine. Okra flowers which are by-products of 
      okra, are rich in polysaccharide, polyphenols and trace elements etc., however, 
      except a few for health tea, most of them were discarded as the waste of 
      resources. In this study, a polysaccharide named AEFP22 was extracted, purified 
      and identified from okra flowers, and its physicochemical property and 
      antioxidant activity were also elucidated. AEFP22, with a molecular weight of 
      2.741 x 10(5) Da, was composed of Rha, GalA and Gal in the ratio of 1: 1.02: 
      0.86. The methylation and nuclear magnetic resonance (NMR) analysis indicated 
      AEFP22 was composed of 
      [2)-alpha-D-Rhap-(1 --> 4)-alpha-D-GalpA-(1 --> 2,4)-alpha-D-Rhap-(1 --> 4)-alpha-D-GalpA-(1] with 
      branch of terminal T-alpha-D-Galp pointed at C4 of 1,2,4-alpha-D-Rhap. The Conge-red 
      test, Atomic force microscope (AFM) and Scanning electron microscope (SEM) 
      further revealed the triple-helical conformation, irregular sheet structure with 
      molecule aggregations of AEFP22. The physicochemical property analysis indicated 
      AEFP22 possessed stable thermal property and exhibited shear-thinning and normal 
      Newtonian fluid in different concentrations, -7.04 mV zeta potential and 
      polymerization phenomenon existed in AEFP22 solution. AEFP22 exhibited good 
      1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability. These results 
      indicated potential utilization of AEFP22 in nutritional food and material 
      application.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhang, Weijie
AU  - Zhang W
AD  - School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, 
      Zhenjiang 212013, China.
FAU - Xiang, Qingfang
AU  - Xiang Q
AD  - School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, 
      Zhenjiang 212013, China.
FAU - Zhao, Jie
AU  - Zhao J
AD  - School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, 
      Zhenjiang 212013, China.
FAU - Mao, Guanghua
AU  - Mao G
AD  - School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 
      301, Zhenjiang 212013, Jiangsu, China.
FAU - Feng, Weiwei
AU  - Feng W
AD  - School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 
      301, Zhenjiang 212013, Jiangsu, China.
FAU - Chen, Yao
AU  - Chen Y
AD  - School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 
      301, Zhenjiang 212013, Jiangsu, China.
FAU - Li, Qian
AU  - Li Q
AD  - School of Food Science and Engineering, Yangzhou University, Huayangxi Rd. 196, 
      Yangzhou 225127, Jiangsu, China.
FAU - Wu, Xiangyang
AU  - Wu X
AD  - School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 
      301, Zhenjiang 212013, Jiangsu, China.
FAU - Yang, Liuqing
AU  - Yang L
AD  - School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, 
      Zhenjiang 212013, China. Electronic address: yangliuqing@ujs.edu.cn.
FAU - Zhao, Ting
AU  - Zhao T
AD  - School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, 
      Zhenjiang 212013, China. Electronic address: zhaoting@ujs.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200331
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antioxidants)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Polyphenols)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Abelmoschus/*chemistry
MH  - Antioxidants/*metabolism
MH  - Dietary Carbohydrates/isolation & purification/metabolism
MH  - Flowers/*chemistry
MH  - Molecular Weight
MH  - Polyphenols/*analysis
MH  - Polysaccharides/*chemistry/*isolation & purification/metabolism
OTO - NOTNLM
OT  - Okra flower polysaccharide
OT  - Physicochemical property analysis
OT  - Structural elucidation
COIS- Declaration of competing interest None. This research did not receive any 
      specific grant from funding agencies in the public, commercial, or not-for-profit 
      sectors.
EDAT- 2020/04/03 06:00
MHDA- 2021/03/13 06:00
CRDT- 2020/04/03 06:00
PHST- 2020/03/07 00:00 [received]
PHST- 2020/03/26 00:00 [revised]
PHST- 2020/03/27 00:00 [accepted]
PHST- 2020/04/03 06:00 [pubmed]
PHST- 2021/03/13 06:00 [medline]
PHST- 2020/04/03 06:00 [entrez]
AID - S0141-8130(20)32822-1 [pii]
AID - 10.1016/j.ijbiomac.2020.03.235 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2020 Jul 15;155:740-750. doi: 
      10.1016/j.ijbiomac.2020.03.235. Epub 2020 Mar 31.