PMID- 3224124
OWN - NLM
STAT- MEDLINE
DCOM- 19890405
LR  - 20190815
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 9
IP  - 5
DP  - 1988 Sep
TI  - Retardation of calcification of bovine pericardium used in bioprosthetic heart 
      valves by phosphocitrate and a synthetic analogue.
PG  - 393-7
AB  - The purpose of this study was to determine if phosphocitrate (PC), a naturally 
      occurring inhibitor of calcification, and its synthetic analogue, 
      N-sulpho-2-amino tricarballylate (SAT), administered either by daily injection or 
      local delivery via Alzet osmotic minipump, could inhibit calcification of 
      glutaraldehyde-preserved bovine pericardium used in bioprosthetic heart valves, 
      subcutaneously implanted in rats. Local drug delivery, but not systemic 
      administration, was effective. PC, administered by Alzet minipump (12 
      mg.kg-1.day-1), inhibited calcification significantly (tissue calcium = 5 +/- 2 
      micrograms/mg dry tissue, mean +/- SEM), compared with untreated or 
      saline-treated controls (89 +/- 9 and 49 +/- 9 micrograms/mg, respectively). SAT, 
      administered by the same route at both the same and a higher molar dosage, was 
      less potent (tissue calcium = 26 +/- 9 micrograms/mg and 17 +/- 5 micrograms/mg, 
      respectively). PC and SAT therapy were not associated with adverse effects. We 
      conclude that locally administered PC and SAT can inhibit intrinsic calcification 
      of bovine pericardium, with PC being more potent.
FAU - Tsao, J W
AU  - Tsao JW
AD  - Department of Pathology, Brigham and Women's Hospital, Boston.
FAU - Schoen, F J
AU  - Schoen FJ
FAU - Shankar, R
AU  - Shankar R
FAU - Sallis, J D
AU  - Sallis JD
FAU - Levy, R J
AU  - Levy RJ
LA  - eng
GR  - HL 24463/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Citrates)
RN  - 0 (Tricarboxylic Acids)
RN  - 2565-87-9 (phosphocitrate)
RN  - 86883-55-8 (N-sulfo-2-aminotricarballylate)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials
MH  - Calcinosis/*prevention & control
MH  - Cattle
MH  - Citrates/*pharmacology
MH  - Growth Plate/drug effects
MH  - *Heart Valve Prosthesis
MH  - Male
MH  - Pericardium/drug effects/*pathology/transplantation
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Tricarboxylic Acids/*pharmacology
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
AID - 0142-9612(88)90002-6 [pii]
AID - 10.1016/0142-9612(88)90002-6 [doi]
PST - ppublish
SO  - Biomaterials. 1988 Sep;9(5):393-7. doi: 10.1016/0142-9612(88)90002-6.