PMID- 32241279 OWN - NLM STAT- MEDLINE DCOM- 20210202 LR - 20210202 IS - 1476-4598 (Electronic) IS - 1476-4598 (Linking) VI - 19 IP - 1 DP - 2020 Apr 2 TI - A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer. PG - 71 LID - 10.1186/s12943-020-01179-5 [doi] LID - 71 AB - BACKGROUND: Colon cancer (CC) is a common malignant cancer. Recently, circFNDC3B was found to exert biological function in multiple cancers. However, it was unclear whether the potential protein encoded by circFNDC3B is involved in carcinogenesis of CC. METHODS: We used Sanger sequence and RNase R digestion assay to confirm the existence of circFNDC3B, and quantitative real-time PCR was used to evaluate the circRNA's expression. Then fluorescence in situ hybridization (FISH) was performed to study location of circFNDC3B. The identification of protein encoded by circFNDC3B was performed using LC-MS/MS. The function of circFNDC3B-218aa on proliferation, invasion and migration were assessed by CCK8 assays, colony formation assays, transwell assays, wound-healing assays and animal experiments. RNA-sequencing and western blot were used to identify the gene regulated by circFNDC3B-218aa. Finally, glucose metabolism-related assays were performed to further investigate function of circFNDC3B-218aa. RESULTS: CircFNDC3B was localized mostly in the cytoplasm, and was decreased in CC cell lines and tissues. The patients with low circFNDC3B expression had a shorter OS (P = 0.0014) than patients with high expression. Moreover, circFNDC3B inhibited the proliferation, invasion and migration of CC cells. Next, we identified that circFNDC3B could encode a novel protein circFNDC3B-218aa. Furthermore, circFNDC3B-218aa, not circFNDC3B, inhibited the proliferation, invasion and migration of CC. Additionally, the in vivo experiments implied that up-regulated circFNDC3B-218aa exhibited an inhibitory effect on CC progression. By RNA-sequencing, western blot and glucose metabolism-related assays, we found that circFNDC3B-218aa inhibited the expression of Snail, and subsequently promoted the tumor-suppressive effect of FBP1 in CC. CONCLUSIONS: The novel circFNDC3B-218aa may serve as a tumor suppressive factor and potential biomarker which may supply the potential therapeutic target for CC. FAU - Pan, Zihao AU - Pan Z AD - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Cai, Jianye AU - Cai J AD - Department of Hepatic Surgery and Liver Transplantation Center, Department of Hepatic Surgery and Liver Transplantation Center, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. AD - Guangdong Key Laboratory of Liver Disease Research, Key Laboratory of Liver Disease Biotherapy and Translational Medicine of Guangdong Higher Education Institutes, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. FAU - Lin, Jiatong AU - Lin J AD - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Zhou, Huinian AU - Zhou H AD - Department of General Surgery, Lanzhou University Second Hospital, Lanzhou, China. FAU - Peng, Jingwen AU - Peng J AD - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Liang, Jinliang AU - Liang J AD - Guangdong Key Laboratory of Liver Disease Research, Key Laboratory of Liver Disease Biotherapy and Translational Medicine of Guangdong Higher Education Institutes, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. FAU - Xia, Long AU - Xia L AD - Department of Hepatic Surgery and Liver Transplantation Center, Department of Hepatic Surgery and Liver Transplantation Center, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. AD - Guangdong Key Laboratory of Liver Disease Research, Key Laboratory of Liver Disease Biotherapy and Translational Medicine of Guangdong Higher Education Institutes, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. FAU - Yin, Qi AU - Yin Q AD - CookGen Biosciences Center, Guangzhou, China. FAU - Zou, Baojia AU - Zou B AD - Department of Hepatobiliary Surgery, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China. FAU - Zheng, Jun AU - Zheng J AD - Department of Hepatic Surgery and Liver Transplantation Center, Department of Hepatic Surgery and Liver Transplantation Center, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. zhengj67@mail2.sysu.edu.cn. AD - Guangdong Key Laboratory of Liver Disease Research, Key Laboratory of Liver Disease Biotherapy and Translational Medicine of Guangdong Higher Education Institutes, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. zhengj67@mail2.sysu.edu.cn. FAU - Qiao, Liang AU - Qiao L AD - Storr Liver Centre, Westmead Institute for Medical Research, University of Sydney at Westmead Hospital, Westmead, NSW, 2145, Australia. liang.qiao@sydney.edu.au. FAU - Zhang, Lei AU - Zhang L AD - Department of Biliary-Pancreatic Surgery, The Third Affiliated Hospital, Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. zhanglei63@mail.sysu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200402 PL - England TA - Mol Cancer JT - Molecular cancer JID - 101147698 RN - 0 (Biomarkers, Tumor) RN - 0 (FNDC3B protein, human) RN - 0 (Fibronectins) RN - 0 (RNA, Circular) RN - 0 (SNAI1 protein, human) RN - 0 (Snail Family Transcription Factors) SB - IM MH - Animals MH - Apoptosis MH - Biomarkers, Tumor/genetics/*metabolism MH - Cell Proliferation MH - Colonic Neoplasms/genetics/metabolism/*pathology MH - *Epithelial-Mesenchymal Transition MH - Female MH - Fibronectins/*genetics MH - *Gene Expression Regulation, Neoplastic MH - Humans MH - Male MH - Mice MH - Mice, Inbred BALB C MH - Mice, Nude MH - Middle Aged MH - Prognosis MH - RNA, Circular/*genetics MH - Snail Family Transcription Factors/genetics/*metabolism MH - Survival Rate MH - Tumor Cells, Cultured MH - Xenograft Model Antitumor Assays PMC - PMC7114813 OTO - NOTNLM OT - Colon cancer OT - FBP1 OT - circFNDC3B OT - circRNA COIS- The authors declare that they have no competing interests. EDAT- 2020/04/04 06:00 MHDA- 2021/02/03 06:00 PMCR- 2020/04/02 CRDT- 2020/04/04 06:00 PHST- 2019/12/02 00:00 [received] PHST- 2020/03/05 00:00 [accepted] PHST- 2020/04/04 06:00 [entrez] PHST- 2020/04/04 06:00 [pubmed] PHST- 2021/02/03 06:00 [medline] PHST- 2020/04/02 00:00 [pmc-release] AID - 10.1186/s12943-020-01179-5 [pii] AID - 1179 [pii] AID - 10.1186/s12943-020-01179-5 [doi] PST - epublish SO - Mol Cancer. 2020 Apr 2;19(1):71. doi: 10.1186/s12943-020-01179-5.