PMID- 32243956
OWN - NLM
STAT- MEDLINE
DCOM- 20201216
LR  - 20201216
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 294
DP  - 2020 Aug 1
TI  - Identification of kisspeptin2 cDNA in the catfish Heteropneustes fossilis: 
      Expression profile, in situ localization and steroid modulation.
PG  - 113472
LID - S0016-6480(19)30457-5 [pii]
LID - 10.1016/j.ygcen.2020.113472 [doi]
AB  - Kisspeptin (Kiss) is considered an upstream regulator of gonadotropin-releasing 
      hormone in mammals but its role in non-mammalian vertebrates is not unequivocally 
      established. In the catfish Heteropneustes fossilis, a 605 bp long cDNA was 
      identified from the brain by cloning as well as by retrieving from the catfish 
      transcriptome database. The open reading frame (ORF, 93-405 bp) codes for a 113 
      amino acids long precursor protein. Homology and phylogenetic analyses showed 
      that the predicted protein belongs to the vertebrate Kiss2 type with a high 
      degree of conservation in the Kiss2-10 region (FNFNPFGLRF). The kiss2 transcripts 
      were expressed highly in the brain and gonads in a dimorphic manner with a female 
      bias. In the brain, kiss2 transcripts showed regional differences with higher 
      expression in the medulla oblongata and forebrain regions. The kiss2 transcripts 
      showed significant seasonal variations with the highest expression in the brain 
      in spawning phase and in the gonads in prespawning phase. The kiss2 transcripts 
      were localized in the brain (nucleus preopticus, habenular nucleus, nucleus 
      recessus posterioris, nucleus recessus lateralis) and stratum periventriculare 
      (radial glial cells) of optic tectum, pituitary and ovary (follicular layer and 
      germinal vesicle). Ovariectomy (1, 2, 3 and 4 weeks) decreased brain kiss2 mRNA 
      levels and a single injection of estradiol-17beta (E(2); 0.5 mug/g body weight) in 3- 
      week ovariectomized (OVX) and sham operated fish resulted in an increase in the 
      transcript levels after 24 h. The E(2) receptor antagonist Tamoxifen (TMX) 
      produced biphasic effects on the kiss2 expression in the dose- response study. 
      TMX inhibited the expression in the OVX fish, but elicited a stimulatory effect 
      in the OVX + E(2)-treated fish. Testosterone (T) decreased, and progesterone 
      (P(4)) inhibited (resting phase) or stimulated (prespawning phase) the transcript 
      level in 3-week OVX fish. In the 3-week sham groups, E(2) increased, and TMX, T 
      and P(4) inhibited the kiss2 transcript levels. The results suggest that Kiss2 is 
      an important regulator of the brain- pituitary- gonadal- endocrine axis, and in 
      habenular and optic tectum functions.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Chaube, R
AU  - Chaube R
AD  - Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 
      221005, India.
FAU - Sharma, S
AU  - Sharma S
AD  - Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 
      221005, India.
FAU - Senthilkumaran, B
AU  - Senthilkumaran B
AD  - Department of Animal Biology, School of Life Sciences, University of Hyderabad, 
      Hyderabad 500046, India.
FAU - Bhat, S G
AU  - Bhat SG
AD  - Centre for Neuroscience, Department of Biotechnology, Cochin University of 
      Science and Technology, Kochi 682022, India.
FAU - Joy, K P
AU  - Joy KP
AD  - Centre for Neuroscience, Department of Biotechnology, Cochin University of 
      Science and Technology, Kochi 682022, India. Electronic address: 
      kpjoybhu@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200331
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (DNA, Complementary)
RN  - 0 (Kisspeptins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Steroids)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - 3XMK78S47O (Testosterone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Catfishes/*genetics
MH  - DNA, Complementary/*genetics
MH  - Estradiol/pharmacology
MH  - Female
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Humans
MH  - Kisspeptins/chemistry/*genetics/metabolism
MH  - Ovariectomy
MH  - Ovary/drug effects/metabolism
MH  - Phylogeny
MH  - Progesterone/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Seasons
MH  - Steroids/*metabolism
MH  - Tamoxifen/pharmacology
MH  - Testosterone/pharmacology
OTO - NOTNLM
OT  - Brain and gonad expression
OT  - Catfish
OT  - Dimorphic and seasonal expression
OT  - Kiss2 cDNA
OT  - Steroid regulation
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/04/04 06:00
MHDA- 2020/12/17 06:00
CRDT- 2020/04/04 06:00
PHST- 2019/08/27 00:00 [received]
PHST- 2020/02/22 00:00 [revised]
PHST- 2020/03/28 00:00 [accepted]
PHST- 2020/04/04 06:00 [pubmed]
PHST- 2020/12/17 06:00 [medline]
PHST- 2020/04/04 06:00 [entrez]
AID - S0016-6480(19)30457-5 [pii]
AID - 10.1016/j.ygcen.2020.113472 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2020 Aug 1;294:113472. doi: 10.1016/j.ygcen.2020.113472. 
      Epub 2020 Mar 31.