PMID- 32245492
OWN - NLM
STAT- MEDLINE
DCOM- 20201201
LR  - 20201201
IS  - 1746-1596 (Electronic)
IS  - 1746-1596 (Linking)
VI  - 15
IP  - 1
DP  - 2020 Apr 3
TI  - A rare face of follicular lymphoma: reverse variant of follicular lymphoma.
PG  - 31
LID - 10.1186/s13000-020-00932-0 [doi]
LID - 31
AB  - BACKGROUND: Reverse Variant of Follicular Lymphoma (RVFL) is one of the rare 
      morphological variants of FL, characterized by dark staining small centrocytes in 
      the center and pale staining large centroblasts at the periphery of the 
      neoplastic follicles. Only rare cases of RVFL have been described to date. The 
      histological appearance of this little known variant of FL may be misinterpreted 
      if pathologists are unaware of its existence. The main purpose of this study is 
      to draw pathologists' attention to such an uncommon growth pattern of FL so that 
      this variant can be correctly recognized and the clinical significance further 
      studied in the future. METHODS: Four cases of FL with unusual morphologic 
      features were evaluated for the expression pattern of CD20, CD10, BCL6, BCL2, 
      CD21, CD23, CD3, CD5, Cyclin D1, IgD and Ki67 by immunohistochemistry. 
      Fluorescence in situ hybridization (FISH) with break-apart probes was performed 
      to detect BCL2 gene rearrangement. RESULTS: All four cases showed distinctive 
      morphologic pattern of RVFL; in addition, each also exemplified unique 
      morphological features. Immunohistochemical stains confirmed the cells in both 
      the central areas and the peripheral cuffs had the same immunophenotypic 
      profiles, contrasting to the FL with marginal zone differentiation in which only 
      the center of the nodules showed expression of CD10. FISH demonstrated BCL2 gene 
      rearrangement in all cases. CONCLUSION: The growth pattern of this rare FL 
      variant may mimic FL with marginal-zone differentiation and other entities 
      including but not limited to marginal zone lymphoma (MZL), progressive 
      transformation of germinal centers (PTGC) and nodular lymphocyte predominant 
      Hodgkin lymphoma (NLPHL). Pathologists should be familiar with this unusual 
      morphological variant to avoid diagnostic pitfalls.
FAU - Maskey, Ninu
AU  - Maskey N
AD  - Department of Hematology, Zhongnan Hospital of Wuhan University, 169 Donghu Road, 
      Wuchang District, Wuhan, Hubei, 430071, People's Republic of China.
FAU - Chen, Qiongrong
AU  - Chen Q
AD  - Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan University 
      Center for Pathology and Molecular Diagnostics, 169 Donghu Road, Wuchang 
      District, Wuhan, 430071, People's Republic of China.
FAU - Liu, Fang
AU  - Liu F
AD  - Department of Pathology, Foshan Hospital, Sun Yat-sen University, 81 North 
      Lingnan Avenue, Chancheng District, Foshan, Guangdong Province, People's Republic 
      of China.
FAU - Liu, Shangqin
AU  - Liu S
AD  - Department of Hematology, Zhongnan Hospital of Wuhan University, 169 Donghu Road, 
      Wuchang District, Wuhan, Hubei, 430071, People's Republic of China. 
      ubeliu@aliyun.com.
FAU - Tian, Sufang
AU  - Tian S
AUID- ORCID: 0000-0002-3025-666X
AD  - Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan University 
      Center for Pathology and Molecular Diagnostics, 169 Donghu Road, Wuchang 
      District, Wuhan, 430071, People's Republic of China. 
      usa2005tiansufang@hotmail.com.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20200403
PL  - England
TA  - Diagn Pathol
JT  - Diagnostic pathology
JID - 101251558
RN  - 0 (BCL2 protein, human)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/analysis
MH  - Female
MH  - Gene Rearrangement
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymph Nodes/*pathology
MH  - Lymphoma, Follicular/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
PMC - PMC7119098
OTO - NOTNLM
OT  - Centroblasts
OT  - Centrocytes
OT  - Follicular dendritic cells meshwork
OT  - Follicular lymphoma
OT  - Reverse variant of follicular lymphoma
COIS- The authors declare that they have no competing interest.
EDAT- 2020/04/05 06:00
MHDA- 2020/12/02 06:00
PMCR- 2020/04/03
CRDT- 2020/04/05 06:00
PHST- 2019/10/11 00:00 [received]
PHST- 2020/02/05 00:00 [accepted]
PHST- 2020/04/05 06:00 [entrez]
PHST- 2020/04/05 06:00 [pubmed]
PHST- 2020/12/02 06:00 [medline]
PHST- 2020/04/03 00:00 [pmc-release]
AID - 10.1186/s13000-020-00932-0 [pii]
AID - 932 [pii]
AID - 10.1186/s13000-020-00932-0 [doi]
PST - epublish
SO  - Diagn Pathol. 2020 Apr 3;15(1):31. doi: 10.1186/s13000-020-00932-0.