PMID- 32247045 OWN - NLM STAT- MEDLINE DCOM- 20210112 LR - 20210112 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 101 DP - 2020 Jun TI - Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment. PG - 205-215 LID - S1050-4648(20)30222-9 [pii] LID - 10.1016/j.fsi.2020.03.054 [doi] AB - The interaction between CD2-associated protein (CD2AP) and CD2 plays a vital role in lymphocyte adhesion and T cells activation in mammals. In this study, a CD2AP gene (GenBank accession number: MK579862; designated as On-CD2AP) was identified from tilapia (Oreochromis niloticus). Sequence analysis showed that On-CD2AP protein shares high similarity with mammals, including three Src homology 3 (SH3) domains, a section of poly proline motif and a coiled coil region. Transcription levels of On-CD2AP were detected in nine tissues of healthy Nile tilapia, and the highest expression levels were detected in the spleen and gill. On-CD2AP were significantly up-regulated in thymus, head kidney and brain after infected by Streptococcus agalactiae, as well as in head kidney leukocytes (HKLs) with LPS and LTA stimulation. Moreover, a section conserved pro-rich motif that are responsible for binding of CD2 to CD2AP were found in the CD2 cytoplasmic sequence of Nile tilapia (On-CD2C). A weak interaction between On-CD2AP and On-CD2C was proved by yeast two-hybrid assay. In addition, the recombinant proteins of CD2AP-His (rOn-CD2AP-His) and GST-CD2C (GST-rOn-CD2C) were obtained through prokaryotic expression system. His pull-down assay showed that rOn-CD2AP-His and GST-rOn-CD2C could bind to each other. These findings indicate that CD2AP is crucial in immune response during S.agalactiae infection, and the mechanism of interaction between CD2AP and CD2 is conservative in Nile tilapia. CI - Copyright (c) 2020 Elsevier Ltd. All rights reserved. FAU - Li, Yuan AU - Li Y AD - Shenzhen Institute of Guangdong Ocean University, Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, Guangdong, China; Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China. FAU - Wang, Bei AU - Wang B AD - Shenzhen Institute of Guangdong Ocean University, Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, Guangdong, China; Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: wong19820204@126.com. FAU - Wang, Zhi-Wen AU - Wang ZW AD - Shenzhen Institute of Guangdong Ocean University, Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, Guangdong, China; Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China. FAU - Huang, Yu AU - Huang Y AD - Shenzhen Institute of Guangdong Ocean University, Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, Guangdong, China; Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. FAU - Jian, Ji-Chang AU - Jian JC AD - Shenzhen Institute of Guangdong Ocean University, Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, Guangdong, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. FAU - Lu, Yi-Shan AU - Lu YS AD - Shenzhen Institute of Guangdong Ocean University, Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, 518120, Guangdong, China; Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: fishdis@163.com. LA - eng PT - Journal Article DEP - 20200401 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Adaptor Proteins, Signal Transducing) RN - 0 (CD2-associated protein) RN - 0 (Cytoskeletal Proteins) RN - 0 (Fish Proteins) SB - IM MH - Adaptor Proteins, Signal Transducing/chemistry/*genetics/*immunology MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Cichlids/*genetics/*immunology MH - Cytoskeletal Proteins/chemistry/*genetics/*immunology MH - Fish Diseases/*immunology MH - Fish Proteins/chemistry/genetics/immunology MH - Gene Expression Profiling/veterinary MH - Gene Expression Regulation/*immunology MH - Immunity, Innate/*genetics MH - Phylogeny MH - Sequence Alignment/veterinary MH - Streptococcal Infections/immunology/veterinary MH - Streptococcus agalactiae/physiology OTO - NOTNLM OT - CD2 OT - CD2-associated protein OT - Immune response OT - Nile tilapia OT - Streptococcus agalactiae OT - T cells activation COIS- Declaration of competing interest We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled. EDAT- 2020/04/05 06:00 MHDA- 2021/01/13 06:00 CRDT- 2020/04/05 06:00 PHST- 2020/03/03 00:00 [received] PHST- 2020/03/23 00:00 [revised] PHST- 2020/03/25 00:00 [accepted] PHST- 2020/04/05 06:00 [pubmed] PHST- 2021/01/13 06:00 [medline] PHST- 2020/04/05 06:00 [entrez] AID - S1050-4648(20)30222-9 [pii] AID - 10.1016/j.fsi.2020.03.054 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2020 Jun;101:205-215. doi: 10.1016/j.fsi.2020.03.054. Epub 2020 Apr 1.