PMID- 32248406
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20211207
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 61
IP  - 3
DP  - 2020 Sep
TI  - FISH karyotype comparison between A(b)- and A-genome chromosomes using 
      oligonucleotide probes.
PG  - 313-322
LID - 10.1007/s13353-020-00555-7 [doi]
AB  - Triticum boeoticum (2n = 2x = 14, A(b)A(b)) contains beneficial traits for common 
      wheat improvement. The discrimination of A(b)-genome chromosomes from A-genome 
      chromosomes is an important step in gene transfer from T. boeoticum to common 
      wheat. In this study, fluorescence in situ hybridization (FISH) analysis using 
      nine oligonucleotide probes revealed high divergence between chromosomes of the 
      common wheat germplasm Crocus and T. boeoticum accession G52. The combination of 
      Oligo-pTa535-HM and Oligo-pSc119.2-HM can differentiate A(b) and A chromosomes 
      within homologous groups 2, 4, 5, and 6; chromosomes 2A(b) and 6A(b) can be 
      identified by using (ACT)(7), (CTT)(7), and (GAA)(7). The probes Oligo-pTa713 and 
      (ACT)(7) can be utilized for the identification of chromosomes 1A(b) and 3A(b), 
      respectively. Probes (CAG)(7) and (CAC)(7) can be applied in the identification 
      of 7A(b). Moreover, probe combinations consisting of Oligo-pTa535-HM and (AAC)(7) 
      with (ACT)(7) or (CTT)(7) and of Oligo-pTa535-HM and Oligo-pTa713 with (CAC)(7) 
      or (CTT)(7) will help discriminate the A(b)-genome chromosomes of T. boeoticum. 
      These probes are being used as potential markers to select common wheat Crocus-T. 
      boeoticum G52 alien chromosome lines. Moreover, FISH patterns are highly 
      divergent between A(b)- and A-genome chromosomes, indicating that obvious 
      chromosome structural variations arose during wheat evolution.
FAU - Feng, Zhen
AU  - Feng Z
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Zhang, Minghu
AU  - Zhang M
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Liu, Xin
AU  - Liu X
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Liang, Dongyu
AU  - Liang D
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Liu, Xiaojuan
AU  - Liu X
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Hao, Ming
AU  - Hao M
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Liu, Dengcai
AU  - Liu D
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Ning, Shunzong
AU  - Ning S
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Yuan, Zhongwei
AU  - Yuan Z
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Jiang, Bo
AU  - Jiang B
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Chen, Xuejiao
AU  - Chen X
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Chen, Xue
AU  - Chen X
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China.
FAU - Zhang, Lianquan
AU  - Zhang L
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China. 
      zhanglianquan1977@126.com.
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 
      611130, China. zhanglianquan1977@126.com.
LA  - eng
GR  - 31671682/the National Natural Science Foundation of China/
GR  - 31671689/the National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200405
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - *Genome, Plant
MH  - *In Situ Hybridization, Fluorescence
MH  - *Karyotyping
MH  - Oligonucleotide Probes/genetics
MH  - Triticum/classification/*genetics
OTO - NOTNLM
OT  - Chromosome identification
OT  - FISH
OT  - Triticum aestivum
OT  - Triticum boeoticum
EDAT- 2020/04/06 06:00
MHDA- 2021/03/09 06:00
CRDT- 2020/04/06 06:00
PHST- 2019/10/13 00:00 [received]
PHST- 2020/03/16 00:00 [accepted]
PHST- 2020/02/26 00:00 [revised]
PHST- 2020/04/06 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
PHST- 2020/04/06 06:00 [entrez]
AID - 10.1007/s13353-020-00555-7 [pii]
AID - 10.1007/s13353-020-00555-7 [doi]
PST - ppublish
SO  - J Appl Genet. 2020 Sep;61(3):313-322. doi: 10.1007/s13353-020-00555-7. Epub 2020 
      Apr 5.